Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes Perinereis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection and Processing
2.2. RNA Library Construction and Meta-Transcriptomic Sequencing
2.3. Sequence Assembly and RNA Virus Discovery
2.4. Genome Structure Prediction and Phylogenetic Analysis
3. Results
3.1. Composition and Diversity of the Perinereis Virome
3.2. Phylogenetic Analysis of the Novel Viruses in the Order Picornavirales
3.2.1. Phylogenetic Analysis of the Novel Viruses in the Family Marnaviridae
3.2.2. Phylogenetic Analysis of the Novel Viruses in Unclassified Picornavirales
3.3. Phylogenetic Analysis of the Novel Viruses in the Family Picobirnaviridae
3.4. Phylogenetic Analysis of the Novel Viruses in Unclassified Bunyavirales
3.5. Genome Structures of the Perinereis Viruses
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Luo, J.; Zhang, F.; Zhou, C.; Meng, F.; Wang, G.; Qiu, L.; Shi, W.; Huang, J.; Dong, X. Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes Perinereis. Vet. Sci. 2024, 11, 273. https://doi.org/10.3390/vetsci11060273
Luo J, Zhang F, Zhou C, Meng F, Wang G, Qiu L, Shi W, Huang J, Dong X. Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes Perinereis. Veterinary Sciences. 2024; 11(6):273. https://doi.org/10.3390/vetsci11060273
Chicago/Turabian StyleLuo, Jingfei, Fan Zhang, Chengyan Zhou, Fanzeng Meng, Guohao Wang, Liang Qiu, Weifeng Shi, Jie Huang, and Xuan Dong. 2024. "Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes Perinereis" Veterinary Sciences 11, no. 6: 273. https://doi.org/10.3390/vetsci11060273