The Latest Prevalence, Isolation, and Molecular Characteristics of Feline Herpesvirus Type 1 in Yanji City, China
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Clinical Samples and Cells
2.2. Isolation and Identification of Viruses
2.3. Transmission Electron Microscopy (TEM)
2.4. Indirect Immunofluorescence Identification
2.5. T-A Cloning and Molecular Epidemiology of FHV-1
2.6. DNA Sequencing and Phylogenetic Analysis
3. Results
3.1. Preliminary Identification of Clinical Samples
3.2. Data Processing of Positive Samples
3.3. Sequence Analysis of the gD Gene
3.4. Phylogenetic Analysis
3.5. Virus Isolation
3.6. Transmission Electron Microscopy
3.7. Indirect Immunofluorescence Identification
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name | Breed | Age (Month) | Clinical Symptoms | Vaccine History | GenBank |
---|---|---|---|---|---|
1 | Maine | 4 | Snot, sneeze | - | OQ587981 |
2 | American Shorthair | 10 | Eye conjunctivitis | ○ | OQ686817 |
3 | British short-haired cat | 6 | Conjunctivitis, sneezing | - | OQ686818 |
4 | Orange cat | 3 | Dispirited, fever | - | OQ686819 |
5 | British short-haired cat | 8 | Fever, eye secretions | - | OQ686820 |
6 | Ragdoll | 2 | Eye and nose secretions | - | OQ686821 |
7 | Ragdoll | 2 | Conjunctivitis, sneezing | - | OQ686822 |
8 | British short-haired cat | 10 | Sneezing | + | OQ686823 |
9 | Chinese Li Hua | 2 | Conjunctivitis, ocular secretions | - | OQ686824 |
10 | Chinese Li Hua | 5 | Cough, conjunctivitis | - | OQ686825 |
11 | Orange cat | 2 | Cough, fever | ○ | OQ686826 |
12 | American Shorthair | 7 | Conjunctivitis | - | OQ686827 |
13 | Pastoral cat | 9 | Dispirited, sneezing | + | OQ686828 |
14 | Siamese | 12 | Conjunctivitis, ocular secretions | ○ | OQ689760 |
15 | British short-haired cat | 5 | Eye and nose secretions | - | OQ689761 |
16 | Garfield | 5 | Conjunctivitis | - | OQ710115 |
17 | Maine | 6 | Conjunctivitis, sneezing | ○ | OQ710116 |
18 | British short-haired cat | 8 | Sneezing | - | OQ710117 |
19 | British short-haired cat | 3 | Eye rot bone | + | OQ710118 |
20 | Pastoral cat | 3 | Conjunctivitis, ocular secretions | - | OQ710119 |
21 | American Shorthair | 4 | Fever, eye and nose secretions | - | OQ710120 |
22 | American Shorthair | 7 | Eye and nose secretions, sneezing | - | OQ710121 |
23 | Maine | 6 | Conjunctivitis, sneezing | ○ | OQ710122 |
24 | Orange cat | 8 | Sneezing | + | OQ710123 |
25 | Garfield | 12 | Conjunctivitis, ocular secretions | ○ | OQ710124 |
26 | British short-haired cat | 2 | Eye and nose secretions, Dispirited | - | OQ710125 |
27 | American Shorthair | 5 | Fever, sneezing and coughing | - | OQ710126 |
28 | Sphynx | 2 | Fever | - | OQ710127 |
29 | Chinese Li Hua | 2 | Fever, sneezing | - | OQ710128 |
30 | Ragdoll | 3 | Conjunctivitis | ○ | OQ710129 |
31 | Maine | 5 | Fever, eye and nose secretions | - | OQ710130 |
32 | British short-haired cat | 7 | Conjunctivitis, ocular secretions | ○ | OQ710131 |
33 | American Shorthair | 4 | Fever, eye discharge | - | OQ710132 |
Prevalence rate = (number of positive samples/total number of subjects) × 100% = 32.2% |
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Yang, M.; Mu, B.; Ma, H.; Xue, H.; Song, Y.; Zhu, K.; Hao, J.; Liu, D.; Li, W.; Zhang, Y.; et al. The Latest Prevalence, Isolation, and Molecular Characteristics of Feline Herpesvirus Type 1 in Yanji City, China. Vet. Sci. 2024, 11, 417. https://doi.org/10.3390/vetsci11090417
Yang M, Mu B, Ma H, Xue H, Song Y, Zhu K, Hao J, Liu D, Li W, Zhang Y, et al. The Latest Prevalence, Isolation, and Molecular Characteristics of Feline Herpesvirus Type 1 in Yanji City, China. Veterinary Sciences. 2024; 11(9):417. https://doi.org/10.3390/vetsci11090417
Chicago/Turabian StyleYang, Meng, Biying Mu, Haoyuan Ma, Haowen Xue, Yanhao Song, Kunru Zhu, Jingrui Hao, Dan Liu, Weijian Li, Yaning Zhang, and et al. 2024. "The Latest Prevalence, Isolation, and Molecular Characteristics of Feline Herpesvirus Type 1 in Yanji City, China" Veterinary Sciences 11, no. 9: 417. https://doi.org/10.3390/vetsci11090417