Isolation, Serovar Identification, and Antimicrobial Susceptibility of Avibacterium paragallinarum from Chickens in China from 2019 to 2020
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling
2.2. A. paragallinarum Isolation
2.3. Serotyping
2.4. Antimicrobial Sensitivity Test
3. Results
3.1. Isolation and Serotyping of A. paragallinarum
3.2. Antimicrobial Susceptibility Testing
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Farm ID | Isolates | Origin | Production Type/Breed of Host | Size of Flocks | Husbandry Systems | Age | Time | Serovar |
---|---|---|---|---|---|---|---|---|
A | 2019/JS03 | Jiangsu | Hy-line brown, layer | 2,000,000 | H-type stacked-cage | 326 | 2019/04 | A |
A | 2019/JS07 | Jiangsu | Hy-line brown, layer | 2,000,000 | H-type stacked-cage | 264 | 2019/04 | A |
B | 2019/JS08 | Jiangsu | Hy-line brown, layer | 1,200,000 | H-type stacked-cage | 241 | 2019/04 | A |
B | 2019/JS15 | Jiangsu | Hy-line brown, layer | 1,200,000 | H-type stacked-cage | 316 | 2019/04 | A |
C | 2019/JS28 | Jiangsu | Hy-line brown, layer | 500,000 | H-type stacked-cage | 207 | 2019/04 | A |
D | 2019/JS31 | Jiangsu | Hy-line brown, layer | 600,000 | H-type stacked-cage | 255 | 2019/07 | B |
D | 2019/JS33 | Jiangsu | Hy-line brown, layer | 600,000 | H-type stacked-cage | 212 | 2019/07 | B |
A | 2019/JS34 | Jiangsu | Hy-line brown, layer | 2,000,000 | H-type stacked-cage | 278 | 2019/07 | B |
A | 2019/JS35 | Jiangsu | Hy-line brown, layer | 2,000,000 | H-type stacked-cage | 278 | 2019/07 | B |
E | 2019/JS36 | Jiangsu | Hy-line brown, layer | 1,000,000 | H-type stacked-cage | 322 | 2019/07 | B |
E | 2019/JS37 | Jiangsu | Hy-line brown, layer | 1,000,000 | H-type stacked-cage | 318 | 2019/07 | B |
E | 2019/JS38 | Jiangsu | Hy-line brown, layer | 1,000,000 | H-type stacked-cage | 42 | 2019/07 | B |
F | 2019/JS39 | Jiangsu | Jing Brown No. 1, layer | 300,000 | H-type stacked-cage | 78 | 2019/07 | B |
F | 2019/JS40 | Jiangsu | Jing Brown No. 1, layer | 300,000 | H-type stacked-cage | 133 | 2019/07 | B |
F | 2019/JS42 | Jiangsu | Jing Brown No. 1, layer | 300,000 | H-type stacked-cage | 311 | 2019/07 | A |
F | 2019/JS44 | Jiangsu | Jing Brown No. 1, layer | 300,000 | H-type stacked-cage | 332 | 2019/07 | C |
G | 2019/JS45 | Jiangsu | Nongda 3, layer | 600,000 | H-type stacked-cage | 276 | 2019/07 | A |
G | 2019/JS46 | Jiangsu | Nongda 3, layer | 600,000 | H-type stacked-cage | 306 | 2019/07 | C |
H | 2019/ NX56 | Ningxia | Hy-line brown, layer parent stock | 300,000 | Net-rearing | 333 | 2019/10 | C |
H | 2019/ NX57 | Ningxia | Hy-line brown, layer parent stock | 300,000 | Net-rearing | 324 | 2019/10 | C |
H | 2019/ NX58 | Ningxia | Hy-line brown, layer parent stock | 300,000 | Net-rearing | 312 | 2019/10 | C |
I | 2019/ HB63 | Hebei | Hy-line brown, layer parent stock | 300,000 | Natural mating cage | 103 | 2019/11 | A |
I | 2019/ HB64 | Hebei | Hy-line brown, layer parent stock | 300,000 | Natural mating cage | 128 | 2019/11 | A |
I | 2019/ HB65 | Hebei | Hy-line brown, layer parent stock | 300,000 | Natural mating cage | 164 | 2019/11 | A |
I | 2019/ HB68 | Hebei | Hy-line brown, layer parent stock | 300,000 | Natural mating cage | 164 | 2019/11 | B |
J | 2020/JS69 | Jiangsu | Hy-line brown, layer | 100,000 | H-type stacked-cage | 273 | 2020/10 | A |
J | 2020/JS70 | Jiangsu | Hy-line brown, layer | 100,000 | H-type stacked-cage | 266 | 2020/10 | C |
F | 2020/JS71 | Jiangsu | Jing Brown No. 1, layer | 300,000 | H-type stacked-cage | 213 | 2020/10 | C |
F | 2020/JS72 | Jiangsu | Jing Brown No. 1, layer | 300,000 | H-type stacked-cage | 298 | 2020/10 | C |
F | 2020/JS73 | Jiangsu | Jing Brown No. 1, layer | 300,000 | H-type stacked-cage | 246 | 2020/10 | C |
B | 2020/JS74 | Jiangsu | Hy-line brown, layer | 1,200,000 | H-type stacked-cage | 323 | 2020/10 | C |
B | 2020/JS75 | Jiangsu | Hy-line brown, layer | 1,200,000 | H-type stacked-cage | 302 | 2020/10 | C |
B | 2020/JS76 | Jiangsu | Hy-line brown, layer | 1,200,000 | H-type stacked-cage | 195 | 2020/10 | C |
G | 2020/JS77 | Jiangsu | Nongda 3, layer | 600,000 | H-type stacked-cage | 311 | 2020/11 | C |
F | 2020/JS78 | Jiangsu | Hy-line brown, layer | 300,000 | H-type stacked-cage | 204 | 2020/11 | C |
G | 2020/JS79 | Jiangsu | Nongda 3, layer | 600,000 | H-type stacked-cage | 362 | 2020/11 | C |
G | 2020/JS80 | Jiangsu | Nongda 3, layer | 600,000 | H-type stacked-cage | 188 | 2020/11 | C |
K | 2020/JS81 | Jiangsu | Hy-line brown, layer | 200,000 | H-type stacked-cage | 76 | 2020/11 | C |
K | 2020/JS82 | Jiangsu | Hy-line brown, layer | 200,000 | H-type stacked-cage | 34 | 2020/11 | C |
L | 2020/JS83 | Jiangsu | Hy-line brown, layer | 300,000 | H-type stacked-cage | 57 | 2020/11 | C |
Strains | MIC (μg/mL) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
VA | AMX | CTF | AMP | COSMMS | DO | OT | FFC | CN | ENR | LS | TAT | |
221 | 0.125 | 0.125 | 0.25 | ≤0.0625 | ≤0.0625 | 2 | 1 | 1 | 0.125 | 0.5 | 32 | 0.25 |
Spross | ≤0.0625 | ≤0.0625 | 0.25 | 0.5 | ≤0.0625 | ≤0.0625 | 0.125 | 2 | 0.25 | 0.125 | 0.5 | 0.5 |
H-18 | 0.25 | 0.25 | 0.25 | 0.25 | ≤0.0625 | 2 | 16 | 0.5 | 0.125 | 1 | 16 | 0.5 |
2019/JS03 | 1 | ≤0.0625 | 0.25 | 0.25 | 16 | 2 | 32 | 8 | 0.5 | 0.25 | 4 | 2 |
2019/JS07 | 2 | 0.25 | 0.5 | 2 | 32 | 4 | 64 | 8 | 2 | 1 | 16 | 16 |
2019/JS08 | 1 | 0.5 | ≤0.0625 | 0.25 | 32 | 4 | 32 | 4 | 0.5 | 0.25 | 16 | 8 |
2019/JS15 | 1 | 0.25 | 0.25 | 0.5 | 32 | 8 | 64 | 1 | 1 | 2 | 8 | 16 |
2019/JS28 | 32 | 8 | 2 | 4 | 64 | 8 | 32 | 16 | 16 | 64 | 16 | 16 |
2019/JS42 | 64 | 1 | 0.5 | 2 | 256 | 32 | 256 | 64 | 64 | 64 | 256 | 256 |
2019/JS45 | 64 | 1 | 0.5 | 4 | 256 | 32 | 128 | 128 | 64 | 64 | 128 | 128 |
2019/HB63 | 4 | 4 | 0.25 | 8 | 256 | 4 | 64 | 32 | 64 | 8 | 32 | 1 |
2019/HB64 | 2 | 1 | 0.5 | 1 | 128 | 16 | 256 | 0.5 | 64 | 64 | 16 | 32 |
2019/HB65 | 2 | 0.25 | 0.25 | 1 | 64 | 32 | 256 | 4 | 64 | 64 | 16 | 32 |
2020/JS69 | 0.25 | 0.125 | 0.125 | 8 | 32 | 1 | 64 | 0.25 | 0.125 | 0.125 | 0.125 | 0.125 |
2019/JS31 | 2 | 0.5 | 1 | 0.5 | 128 | 8 | 128 | 4 | 1 | 8 | 128 | 32 |
2019/JS33 | 8 | 2 | 1 | 0.5 | 256 | 4 | 128 | 8 | 1 | 8 | 128 | 16 |
2019/JS34 | 16 | 0.5 | 0.25 | 0.5 | 256 | 8 | 256 | 4 | 1 | 1 | 64 | 32 |
2019/JS35 | 0.5 | 1 | 0.125 | 0.125 | 32 | 8 | 128 | 1 | 1 | 4 | 4 | 2 |
2019/JS36 | 0.0625 | 0.0625 | 0.125 | 0.125 | 128 | 16 | 128 | 32 | 1 | 0.5 | 8 | 2 |
2019/JS37 | 2 | 2 | 2 | 4 | 256 | 8 | 256 | 32 | 8 | 16 | 32 | 8 |
2019/JS38 | 1 | 1 | 0.5 | 1 | 128 | 8 | 128 | 16 | 1 | 2 | 8 | 4 |
2019/JS39 | 4 | 2 | 0.5 | 0.25 | 64 | 8 | 128 | 32 | 1 | 4 | 2 | 4 |
2019/JS40 | 8 | 0.25 | 1 | 16 | 64 | 8 | 64 | 16 | 1 | 1 | 16 | 4 |
2019/HB68 | 2 | 0.5 | 0.125 | 0.125 | 64 | 4 | 64 | 0.5 | 1 | 0.5 | 8 | 4 |
2019/JS44 | 0.125 | ≤0.0625 | 0.25 | 0.0625 | 32 | 2 | 64 | 0.25 | 0.5 | 0.5 | 2 | 0.5 |
2019/JS46 | 0.125 | 0.125 | 4 | 0.0625 | 16 | 1 | 64 | 0.125 | 64 | 64 | 4 | 1 |
2019/NX56 | 2 | 2 | 0.5 | 2 | 8 | 1 | 4 | 0.25 | 0.5 | 1 | 0.125 | 0.5 |
2019/NX57 | 0.5 | 0.25 | 0.125 | 0.125 | 16 | 0.5 | 8 | 0.5 | 0.25 | 16 | 4 | 1 |
2019/NX58 | 0.5 | 0.0625 | 0.0625 | 0.5 | 16 | 4 | 16 | 4 | 0.0625 | 0.125 | 2 | 0.0625 |
2020/JS70 | 2 | 1 | 0.25 | 0.5 | 32 | 8 | 128 | 2 | 0.125 | 0.0625 | 0.0625 | 0.5 |
2020/JS71 | 8 | 2 | 0.125 | 0.25 | 64 | 8 | 128 | 0.5 | 2 | 0.5 | 8 | 16 |
2020/JS72 | 1 | 0.125 | 0.5 | 2 | 16 | 0.125 | 128 | 0.25 | 0.5 | 0.5 | 1 | 0.125 |
2020/JS73 | 8 | 0.0625 | 0.0625 | 0.25 | 64 | 8 | 128 | 1 | 2 | 0.5 | 8 | 16 |
2020/JS74 | 4 | 2 | 0.25 | 4 | 16 | 2 | 256 | 0.25 | 4 | 0.5 | 8 | 1 |
2020/JS75 | 0.125 | 0.0625 | 0.5 | 0.125 | 16 | 0.25 | 256 | 4 | 0.5 | 0.5 | 0.5 | 0.5 |
2020/JS76 | 2 | 0.25 | 0.25 | 4 | 32 | 4 | 128 | 0.5 | 4 | 0.5 | 8 | 4 |
2020/JS77 | 1 | 0.0625 | 0.0625 | 0.125 | 16 | 0.25 | 128 | 0.25 | 2 | 0.5 | 0.5 | 1 |
2020/JS78 | 0.25 | 0.0625 | 0.25 | 0.25 | 64 | 8 | 128 | 0.125 | 0.5 | 0.5 | 4 | 0.125 |
2020/JS79 | 0.125 | 0.125 | 0.25 | 1 | 64 | 2 | 128 | 0.125 | 8 | 0.125 | 2 | 0.125 |
2020/JS80 | 0.5 | 0.125 | 0.125 | 0.125 | 64 | 4 | 256 | 0.0625 | 2 | 0.25 | 1 | 2 |
2020/JS81 | 0.25 | 0.0625 | 0.0625 | 0.125 | 16 | 2 | 256 | 0.5 | 2 | 0.25 | 0.5 | 0.125 |
2020/JS82 | 0.25 | 0.125 | 0.125 | 0.125 | 64 | 8 | 128 | 0.25 | 2 | 0.5 | 8 | 0.125 |
2020/JS83 | 4 | 8 | 0.125 | 0.125 | 64 | 2 | 128 | 0.125 | 16 | 2 | 1 | 0.125 |
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Guo, M.; Chen, X.; Zhang, H.; Liu, D.; Wu, Y.; Zhang, X. Isolation, Serovar Identification, and Antimicrobial Susceptibility of Avibacterium paragallinarum from Chickens in China from 2019 to 2020. Vet. Sci. 2022, 9, 27. https://doi.org/10.3390/vetsci9010027
Guo M, Chen X, Zhang H, Liu D, Wu Y, Zhang X. Isolation, Serovar Identification, and Antimicrobial Susceptibility of Avibacterium paragallinarum from Chickens in China from 2019 to 2020. Veterinary Sciences. 2022; 9(1):27. https://doi.org/10.3390/vetsci9010027
Chicago/Turabian StyleGuo, Mengjiao, Xiufang Chen, Hao Zhang, Donghui Liu, Yantao Wu, and Xiaorong Zhang. 2022. "Isolation, Serovar Identification, and Antimicrobial Susceptibility of Avibacterium paragallinarum from Chickens in China from 2019 to 2020" Veterinary Sciences 9, no. 1: 27. https://doi.org/10.3390/vetsci9010027