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Article

A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production

by
Nang Nwet Noon Kham
1,
Somsay Phovisay
1,
Kridsada Unban
2,
Apinun Kanpiengjai
3,
Chalermpong Saenjum
4,
Saisamorn Lumyong
5,
Kalidas Shetty
6 and
Chartchai Khanongnuch
5,7,8,*
1
Division of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand
2
Division of Food Science and Technology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand
3
Division of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
4
Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
5
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
6
Global Institute of Food Security and International Agriculture (GIFSIA), Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA
7
Research Center for Multidisciplinary Approaches to Miang, Multidisciplinary Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand
8
Research Center for Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand
*
Author to whom correspondence should be addressed.
J. Fungi 2024, 10(4), 243; https://doi.org/10.3390/jof10040243
Submission received: 25 December 2023 / Revised: 28 February 2024 / Accepted: 19 March 2024 / Published: 23 March 2024
(This article belongs to the Section Fungi in Agriculture and Biotechnology)
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Abstract

This study aims to utilize the microbial resources found within Laphet-so, a traditional fermented tea in Myanmar. A total of 18 isolates of thermotolerant yeasts were obtained from eight samples of Laphet-so collected from southern Shan state, Myanmar. All isolates demonstrated the tannin tolerance, and six isolates were resistant to 5% (w/v) tannin concentration. All 18 isolates were capable of carboxy-methyl cellulose (CMC) degrading, but only the isolate DK showed ethanol production at 45 °C noticed by gas formation. This ethanol producing yeast was identified to be Cyberlindnera rhodanensis based on the sequence analysis of the D1/D2 domain on rRNA gene. C. rhodanensis DK produced 1.70 ± 0.01 U of thermostable extracellular β-glucosidase when cultured at 37 °C for 24 h using 0.5% (w/v) CMC as a carbon source. The best two carbon sources for extracellular β-glucosidase production were found to be either xylose or xylan, with β-glucosidase activity of 3.07–3.08 U/mL when the yeast was cultivated in the yeast malt extract (YM) broth containing either 1% (w/v) xylose or xylan as a sole carbon source at 37 °C for 48 h. The optimal medium compositions for enzyme production predicted by Plackett–Burman design and central composite design (CCD) was composed of yeast extract 5.83 g/L, peptone 10.81 g/L and xylose 20.20 g/L, resulting in a production of 7.96 U/mL, while the medium composed (g/L) of yeast extract 5.79, peptone 13.68 and xylan 20.16 gave 9.45 ± 0.03 U/mL for 48 h cultivation at 37 °C. Crude β-glucosidase exhibited a remarkable stability of 100%, 88% and 75% stable for 3 h at 35, 45 and 55 °C, respectively.
Keywords: Laphet; thermotolerant yeast; β-glucosidase; carbon sources; Plackett–Burman design; Cyberlindnera rhodanensis Laphet; thermotolerant yeast; β-glucosidase; carbon sources; Plackett–Burman design; Cyberlindnera rhodanensis

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MDPI and ACS Style

Kham, N.N.N.; Phovisay, S.; Unban, K.; Kanpiengjai, A.; Saenjum, C.; Lumyong, S.; Shetty, K.; Khanongnuch, C. A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production. J. Fungi 2024, 10, 243. https://doi.org/10.3390/jof10040243

AMA Style

Kham NNN, Phovisay S, Unban K, Kanpiengjai A, Saenjum C, Lumyong S, Shetty K, Khanongnuch C. A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production. Journal of Fungi. 2024; 10(4):243. https://doi.org/10.3390/jof10040243

Chicago/Turabian Style

Kham, Nang Nwet Noon, Somsay Phovisay, Kridsada Unban, Apinun Kanpiengjai, Chalermpong Saenjum, Saisamorn Lumyong, Kalidas Shetty, and Chartchai Khanongnuch. 2024. "A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production" Journal of Fungi 10, no. 4: 243. https://doi.org/10.3390/jof10040243

APA Style

Kham, N. N. N., Phovisay, S., Unban, K., Kanpiengjai, A., Saenjum, C., Lumyong, S., Shetty, K., & Khanongnuch, C. (2024). A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production. Journal of Fungi, 10(4), 243. https://doi.org/10.3390/jof10040243

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