Control of Peach Brown Rot Disease Produced by Monilinia fructicola and Monilinia laxa Using Benzylidene-Cycloalkanones
Abstract
:1. Introduction
2. Materials and Methods
2.1. General Chemistry
2.2. Assessment of Antifungal Capacity
2.2.1. Fungal Isolate and Growing Conditions
2.2.2. Effect of the Benzylidene-Cycloalkanones Compounds on the Mycelial Growth on Monilinia laxa
2.2.3. Effect of the Compounds on Conidial Germination of Monilinia fructicola
2.2.4. In Vivo Antifungal Effects of Hybrids Based on Dihydrocarvone (B, C and G) on Inoculated Nectarines Fruits
2.3. Statistical Analysis
2.4. Molecular Docking and Prediction of the Physicochemical Properties
3. Results and Discussion
3.1. Antifungal Activity of Dihydrocarvone and Its Hybrid Derivatives
3.2. In Vivo Antifungal Effects of Hybrids Based on Dihydrocarvone (B, C and G) on Inoculated Nectarine Fruits
3.3. Molecular Docking of Benzylidene-Cycloalkanones B, G and C
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | EC50 (µg/mL) ± SD a | Lipophilicity Log P | ||
---|---|---|---|---|
M. fructicola d | M. laxa | Conidia M. fructicola e | ||
A | >250 | >250 | >250 | 2.51 |
B | >250 | 8.38 ± 0.68 | <10 | 4.02 |
C | 15.7 ± 1.33 | 31.46 ± 2.82 | <10 | 3.61 |
D | >250 | 27.30 ± 0.04 | 10 | 3.60 |
E | 39.64 ± 1.13 | 42.49 ± 1.04 | <10 | 4.01 |
F | 23.17 ± 1.22 | 10.88 ± 2.82 | <10 | 4.01 |
G | 58.03 ± 0.74 | 55.97 ± 0.98 | <10 | 3.98 |
H | 47.79 ± 0.88 | 7.57 ± 0.48 | >250 | 3.96 |
I | 18.13 ± 0.0 | 5.06 ± 1.05 | <10 | 3.84 |
Control A b | 9.19 ± 0.0 | 10.56 ± 0.0 | <10 | - |
Control B c | 10.55 ± 1.74 | 13.64 ± 1.38 | 110 ± 1.09 | - |
Disease Incidence Percentage of Peach Brown Rot Disease (%) ± SD [*] | |||||
---|---|---|---|---|---|
Treatments | 1 dpi | 2 dpi | 3 dpi | 4 dpi | 5 dpi |
Negative Control | 0 ± 0.0 b | 0 ± 0.0 e | 0 ± 0.0 e | 0 ± 0.0 c | 0± 0.0 b |
Inoculum Control | 6 ± 3.1 a | 31 ± 13 c | 100 ± 0.0 d | 100 ± 0.0 a | 100 ± 0.0 a |
BC1000® Dust | 0 ± 0.0 b | 44 ± 10 d | 69 ± 13 b | 100 ± 0.0 a | 100 ± 0.0 a |
B | 0 ± 0.0 b | 38 ± 14 c | 88 ± 14 c | 88 ± 14 b | 100 ± 0.0 a |
C | 0 ± 0.0 b | 13 ± 3.0 a | 31 ± 13 a | 88 ± 10 b | 94 ± 13 a |
G | 0 ± 0.0 b | 25 ± 0.0 b | 56 ± 10 b | 100 ± 0.0 a | 100 ± 0.0 a |
Compound | Binding Energy (Kcal/mol) |
---|---|
B | −5.3 |
C | −7.1 |
G | −6.6 |
CBE | −7.2 |
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Madrid, A.; Silva, V.; Reyes, C.; Werner, E.; Besoain, X.; Montenegro, I.; Muñoz, E.; Díaz, K. Control of Peach Brown Rot Disease Produced by Monilinia fructicola and Monilinia laxa Using Benzylidene-Cycloalkanones. J. Fungi 2024, 10, 609. https://doi.org/10.3390/jof10090609
Madrid A, Silva V, Reyes C, Werner E, Besoain X, Montenegro I, Muñoz E, Díaz K. Control of Peach Brown Rot Disease Produced by Monilinia fructicola and Monilinia laxa Using Benzylidene-Cycloalkanones. Journal of Fungi. 2024; 10(9):609. https://doi.org/10.3390/jof10090609
Chicago/Turabian StyleMadrid, Alejandro, Valentina Silva, Constanza Reyes, Enrique Werner, Ximena Besoain, Iván Montenegro, Evelyn Muñoz, and Katy Díaz. 2024. "Control of Peach Brown Rot Disease Produced by Monilinia fructicola and Monilinia laxa Using Benzylidene-Cycloalkanones" Journal of Fungi 10, no. 9: 609. https://doi.org/10.3390/jof10090609