Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
Abstract
:1. Introduction
2. Material and Methods
2.1. Exploration of Candidate Proteins
2.2. Bioinformatics Prediction Programs
2.3. Investigation of Amino Acid Sequences
2.4. Alignment of the Amino Acid Sequences of P. brasiliensis and P. lutzii
2.5. Identification of Conserved Peptides from Protein Sequences of P. brasiliensis and P. lutzii
2.6. Prediction of Antigenic and Immunogenic T Cell Epitope
2.7. Prediction of IFN-γ-inducing MHC Class II Binders
2.8. Validation Screening
3. Results
3.1. Selected Articles and Protein Inclusion Criteria
3.2. Analysis of Proteins Based on Their Conserved Domain Database
3.3. Similarity Analysis between the Proteins Selected for P. brasiliensis and P. lutzii
3.4. Prediction of IFN-γ inducing MHC Class II Binders
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Study, Year (Reference) | Study of Methodology | Upregulated Proteins |
---|---|---|
Alegre et al. [23] 2014 | Genome | Glycosyl hydrolase. |
Do Amaral et al. [24] 2019 | LC–MS/MS | Ligand RNA, fructose bisphosphate aldolase, nucleic acid ligand, phosphoglycerate kinase, perixosomal catalase. |
Araújo et al. [25] 2017 | NanoUPLC–MS | Shock protein SSB1, glucan synthase. |
Araújo et al. [26] 2019 | NanoUPLC–MS | Gp 43, pyruvate dehydrogenase, ATP-citrate synthase, succinyl CoA ligase, Shock protein (Hsp 90, HPs 70, Hps 88, Hps 30, Hsp 7, Hsp 70, Hsp 75), alcohol dehydrogenase, pyruvate dehydrogenase. Cap 20, progesterone ligand, regulatory myosin cdc 4, mitochondrial perodoxin PRX1, DNA ligand, shock protein 88, serine phosphatase, threonine, protein F beta acin, enolase, triosphosphate isomerase, carbonic anhydrase, vacuolar protease 4, perodoxin, acetamidase. |
Baeza, et al. [27] 2017 | NanoUPLC–MS | GlucosaminE-6-phosphate-deaminase, phosphoacetilglucosamina mutase, isocitrate mutase, isocitrate liase, malate synthase, 3-hydroxybutyryl-CoA dehydrogenase, 3-Cetoacyl-CoA thiolase, Acyl-CoA dehydrogenase, Acyl-Coenzyme A oxidase, Enoyl-CoA hydratase, short-chain dehydrogenase, carnitine, adenosylomocysteinase, threonine dehydrogenase, methylcrotoyl-CoA carboxilase beta, methylmalonate semialdehyde dehydrogenase, catalase. |
Castilho et al. [28] 2014 | LC–MS/MS | RAC, vacuolar protease, 26 S regulatory subunit, palmitoyl thioesterase, protein associated with a pathogenesis. Cap 20, ligante de progesterona, regulatory myosin cdc 4, mitochondrial perodoxin PRX1, DNA ligand, shock protein 88, phosphatase serine treonine, actin F protein beta, enolase, triophosphate isomerase, carbonic anhydrase, vacuolar protease 4, perodoxin, acetamidase. |
Chaves et al. [29] 2019 | NanoUPLC–MS | Thioredoxin, superoxide dismutase (SOD Fe+, SOD Cu +), serina protease, mitochondrial acetonitate hydratase, histidin kinase, perixossomal hydratase, shock protein (Hsp 90, Hsp 88, Hsp 70, Hsp 30). |
De Curcio et al. [30] 2017 | NanoUPLC–MS | Carrier ATP/ADP, Plasma ATPpase, YOP1 protein, phosphate mitochondrial, osmosensor protein, ologossacaryl transferase, alpha-1,2-mannosyltransferase KTR1, dolichol-phosphato aminotransferase, phosphoinositide phosphate, oxoisovalerate dehydrogenase beta, serine-3-dehydrogenase. |
Chaves et al. [31] 2017 | NanoUPLC–MS | Carrier ATP/ADP, plasma ATPase, YOP1 protein, mitochondrial phosphate, osmosis protein, oligosaccaccharyl transferase, alpha-1.2-mannosyltransferase KTR1, dolichol-phosphate aminotransferase, phosphoinositide phosphate, oxoisovalerate dehydrogenase 3 |
Desjardins et al. [32] 2011 | Genome | Chitin synthase, Shock protein (Hsp 60, Hsp, 88, Hsp 90, Hsp 7, Hsp 70), mycoserosic acid synthase, acyl transferase, Tioredoxina, perixosomal hydratase, histidine kinase, MATA_HMG-box, Interalpha-trypsin, methyltransferase, N-acetyltransferase, peptidase, transketolase, glucoamylase, PADG_11448, PADG_01788. |
De Oliveira, et al. [33] 2018 | LC–MS/MS | Phosphoglycerato kinase, glucose-6-phosphasto isomerase, phosphomannomutase, triosephosphate isomerase, homogentified 1,2-dioxigenase, alpha-ATPase subunit, 12-oxophytodienoate reductase, PAAG_00340, ATP citrate synthase. |
Lima et al. [34] 2014 | NanoUPLC–MS | 4-hydroxylphenolpyruvate dehydrogenase, alanine-glyoxylate aminotransferase, cysteine dioxygenase, aspartate aminotransferase, choline dehydrogenase, glutamate decarboxylase, methylcrotonoyl-CoA carboxylase, sorbitol SOU2, inosine-dehydrogenase-5-monophosphate, fumarylacetoacetate hydrolase, WD40, ATPase, calnexin, complet T subunit T epsilon, tetraclycine. |
Munhoz, et al. [35] 2016 | Genome | 3-hydroxyantaranilate 3,4 dioxygenase, 1,3 glucanase, BUD 32 kinase, glucan 1,3 glucosidase, aminopeptidase M18, serine protease, LOL, cation efflux, RING, phosphatidyl inositol, PADG_00954, thioredoxin, amino acid permease, acyl-CoA dehydrogenase, 3-ketoacyl reductase, transferrin peptidase. |
Oliveira et al. [36] 2016 | NanoUPLC–MS | Epsilon protein, Rad24, ARF GTPase. |
Parente, et al. [37] 2011 | LC–MS/MS | 2-nitropropane dioxygenase, hydroxyacilglutathione hydrolase, L-threonine-3-dehydrogenase, spermidine synthase, glucokinase, pyruvate dehydrogenase, component X protein, nucleoside-diphosphate epimerase, thioredoxin, pentafunctional AROM, adenisulfate kinase, cytochromo c, anikirina, ubiquitin E1, citocromo C, phosophoglycerate kinase, D-hexose-6-phosphate epimerase, hydrolipoyl dehydrogenase, carnitine O acetyltransferase, perixosomal catalase. |
Parente, et al. [38] 2013 | MALDI–MS/MS | Thioredoxin, protein Y20, aldehyde dehydrogenase, shock protein (Hsp 30, Hsp 30, Hsp 70, Hsp 88, SSB1), malate dehydrogenase, methylcitrate synthase, co-chaperone mitochondrial GrpE, 6-phosphogluconolactonase, xanthine phosophoribosyl transferase, aldehyde dehydrogenase, aminoacyl-tRNA-synthase, oxidoreductase, oxalocrotonate tautomerase, metalloproteins, formamidase. |
Parente-Rocha et al. [39] 2015 | Nano-ESI-UPLC–MS | Phosphoglucomutase, hydrolipoyl-dehydrogenase, succinate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, 4-hydroxyfenylpyruvate dioxigenase, vacuolar aminopeptidase, carboxypeptidase Y, aspartyl protease, protepian Y20, monitiol glutaredoxin, thioredoxin, cytochromo c peroxidase, Superoxido dismutase Cu/Zn, glucosamine-frutose-6-phosphato, ATP synthase F1F0, NADP glutamato dehydrogenase, gama glutamyltranspeptidase. |
Pigosso et al. [40] 2013 | MALDI- MS/MS | L-threonine-3-dehydrogenase, 1,2 diyidroxi-3-keto-5-methyllopententene-dioxigenase, 2,5-diceto-D-gluconic acid reductase A, glutathione reductase, 27 KDa, glycoprotein mitochondrial, DNA ligand, corusmate mutase, formamidase fator-1-alpha, phosphoenolpiruvato carboxikinase, 12-oxophitodienate reductase, citrate synthase, gi295666522. |
Pigosso et al. [41] 2017 | NanoUPLC–MS | Formamidase, carnityl-CoA dehydratase, acil-CoA dehydratase, GABA permease, integral membrane protein, 12-oxophytodinoate reductase, ABB effux, dienetaelone hydrolase, cysteine protease PalB, xanthine dehydrogenase, phosphotransferase, PADG_00675, treonina dehydrogenase, glioxilase, acil-CoA dehydrogenase, metallohydrolase. |
Rezende et al. [42] 2011 | MALDI- MS/MS | Mitochondrial peroxiredoxin, mannitol-1-phosphato-5-dehydrogenase, aldehyde dehydrogenase, ciclofilin, cofilin, protein G, trompomyosin, hydrolase, phosphoglycerate kinase, cobalamin, phosphoglucomutase, acetylmomoserine(tiol)-liase. |
Tamayo et al. [43] 2016 | Genoma | Superoxide dismutase (SOD, SOD Cu/Zn). |
Tashima et al. [44] 2015 | LC–MS/MS | Alpha -tubulin. |
Tomazetti, et al. [45] 2019 | LC–MS/MS | 1,6 Glyceradeído-3-phosphate-dehydrogenase, Aqualysin, Fructose-1,6-biphosphate aldolase, enolase, Superoxide dismutase Fe+, thioredoxin reductase, succinil-CoA ligase subunidade beta, methyl-2-citrate-synthase, cytochromo c1 mitochondrial heme, Hsp 30, Hsp 70, cytocromo C peroxidase, oxirredutase, Ras-2, methylcitrate dehydrogenase, thioredoxin, 5-aminolevulinato synthase, serine protease, RPA3, ATPase, Profilin, reductase ferric, S-adenosylmethionine dehydrogenase-dependent-2-methyltransferase beta, pyruvate dehydrogenase subunit E1 beta, dyhydrolipoamide acetyl-transferase, glucosamine-fructose-6-phosphate, GTPase RhoA, receptor endossomal Erp3, Shock protein (Hsp020, Hsp 70), perixosomal, citochromo c peroxidase, phosphoesterase, cofilin, estrictosidin synthase, destrin, RRM-Srp1p. |
Tristão, et al. [46] 2015 | Proteomic | Superoxide dismutase (SOD, SOD Cu/Zn, SOD cytosolic, SOD cytosolic Cu/Zn), laccase 1, L-ascorbitate oxidase, laccase IV, cupredoxin, alcohol dehydrogenase, class II aldolase, PADG_00743, mannose-6-phosphate isomerase, peptidase M1, copper carrier ATPase, heavy metal ATPase, urease, D-arabinose-1-dehydrogenase, calcium carrier, carrier CCC1, calcium carrier, iron and magnesium, cytochromo, ypt5 binding GTPase, mitochondrial porin, SEC62 protein, chpA protein, binding GTPase ypt7, chaperone, ECM33 precursor protein, extracellular matrix component, GTPase sar1, G2/M RNA ligand, iron carrier, vacuolar protein, Ras, clatrin, beta-glucosidase, endosome carrier. |
Weber, et al. [47] 2012 | MALDI-Q-TOF MS | Aminotransferase, fumarylacetoacetase, beta-glycosidase, glycosyl hydrolase, Grp1p, peptidyl-propyl-cis-trans-isomerase A2, disulfide isomerase Pdi1. Enolase, Hsp 10, malate dehydrogenase, serine hydroxylmethyltransferase. |
Protein | Identification | E-Value | Identity | |
---|---|---|---|---|
Pb18 | Pl 01 | |||
Carrier ADP/ATP | PAAG_08620 | 0.0 | 100% | 97% |
Acyl-CoA dehydrogenase | PAAG_03116 | 0.0 | 98% | 100% |
Acyl-CoA dehydrogenase | PADG_06805 | 0.0 | 100% | 98% |
Acyl CoA dehydrogenase | PADG_07604 | 0.0 | 100% | 97% |
Acyl CoA hydratase | PAAG_06309 | 0.0 | 97% | 100% |
Actin F protein subunit uptake protein | PADG_07756 | 0.0 | 100% | 100% |
Alcohol dehydrogenase | PADG_01174 | 0.0 | 100% | 97% |
Alpha-1,2 mannosyltransferase | PAAG_02462 | 0.0 | 100% | 98% |
Alpha-1,2 mannosyltransferase KTR1 | PAAG_07238 | 0.0 | 97% | 100% |
Adenosillomocysteine | PADG_02859 | 0.0 | 99% | 100% |
Aminotransferase | PAAG_03045 | 0.0 | 100% | 98% |
Aminotransferase | PAAG_00053 | 0.0 | 98% | 100% |
ATP citrate synthase | PADG_04993 | 0.0 | 100% | 98% |
ATP_dependent 26S on proteasome regulation | PAAG_01926 | 0.0 | 99% | 100% |
Calnexin | PAAG_07037 | 0.0 | 95% | 100% |
Carrier ADP/ATP | PAAG_08620 | 0.0 | 100% | 97% |
Carrier calcium | PAAG_07762 | 0.0 | 100% | 98% |
Carnitil CoA dehydrogenase | PADG_05773 | 0.0 | 100% | 97% |
Catalase | PAAG_01454 | 0.0 | 100% | 98% |
Catalase | PADG_00324 | 0.0 | 98% | 100% |
Catalase | PAAG_01943 | 0.0 | 99% | 99% |
Chitin synthase VII class | ABV31248.1 | 0.0 | 98% | 99% |
1,3 ketoacyl-CoA lyase | PADG_07365 | 0.0 | 100% | 98% |
3-ketoacyl reductase | PADG_01943 | 0.0 | 100% | 95% |
Citrate synthase | PAAG_08075 | 0.0 | 98% | 100% |
Colin acetyltransferase | PADG_07023 | 0.0 | 100% | 98% |
Gamma subunit complex T | PAAG_07165 | 0.0 | 99% | 100% |
Corismate mutase | PAAG_05198 | 0.0 | 98% | 100% |
Dehydrogenase | PADG_07369 | 0.0 | 100% | 98% |
Dihydrolopiol | PAAG_03330 | 0.0 | 97% | 100% |
Dihydrolopiol dehydrogenase | PAAG_06494 | 0.0 | 100% | 97% |
Dolichol-phosphate mannosyltransferase | PAAG_01874 | 0.0 | 98% | 100% |
Enoyl coenzyme crontonase | PADG_01209 | 0.0 | 100% | 97% |
Formamidase | PAAG_03333 | 0.0 | 99% | 98% |
Formamidase | PADG_06490 | 0.0 | 100% | 97% |
Fumarylacetoacetoato hydrolase | PAAG_00869 | 0.0 | 98% | 100% |
Glutamyl transferase range | PADG_01479 | 0.0 | 97% | 100% |
Glutamate dehydrogenase | PADG_04516 | 0.0 | 100% | 98% |
Glucan synthase | PADG_07373 | 0.0 | 97% | 100% |
Glycosamine-6-phosphate deaminase | PADG_00401 | 0.0 | 100% | 97% |
Hydratase mitochondrial acetones | PAAG_00845 | 0.0 | 100% | 99% |
Hydroacylgluthatione hydrolase | PAAG_02548 | 0.0 | 98% | 100% |
4-Hydroxyfenylpyruvate dioxygenase | PAAG_08468 | 0.0 | 99% | 100% |
Hexose-6-phosphato epimerase | PADG_03243 | 0.0 | 98% | 100% |
Hydratase | PADG_11845 | 0.0 | 99% | 100% |
Hydroxyl-CoA dehydrogenase | PADG_01228 | 0.0 | 100% | 98% |
Hydroacylglutathione hydrolase | PAAG_02548 | 0.0 | 98% | 100% |
Histidin kinase | PADG_11468 | 0.0 | 100% | 96% |
Hypothetical protein | PAAG_02761 | 0.0 | 94% | 100% |
Hypothetical protein | PADG_01788 | 0.0 | 98% | 100% |
Hypothetical protein | PADG_01788 | 0.0 | 98% | 100% |
Interalpha trypsin | PADG_06178 | 0.0 | 100% | 96% |
Inosine-S-monophosphate dehydrogenase MD2 | ||||
Isocitrate lyase | PAAG_04542 | 0.0 | 98% | 99% |
Isocitrate lyase | PAAG_06951 | 0.0 | 100% | 98% |
3-isopropyl dehydrogenase | PAAG_05328 | 0.0 | 97% | 100% |
L-threonine dehydrogenase | PAAG_00966 | 0.0 | 99% | 100% |
Laccase | PADG_06196 | 0.0 | 100% | 96% |
Mallate dehydrogenase | PAAG_00053 | 0.0 | 98% | 100% |
Mallate synthase, glyoxysomal | PADG_04702 | 0.0 | 99% | 97% |
Mallate synthase, glyoxysomal | PADG_04702 | 0.0 | 99% | 97% |
Mannitol-1-phosphate dehydrogenase | PAAG_06473 | 0.0 | 100% | 98% |
Methyltransferase | PADG_01183 | 0.0 | 100% | 94% |
Methyltransferase | PAAG_09014 | 0.0 | 94% | 100% |
Methylcronotonoil-CoA carboxylase | PAAG_04103 | 0.0 | 98% | 100% |
Methilmalonate-semialdehyde dehydrogenase | PAAG_07036 | 0.0 | 98% | 100% |
Metalo hydrolase | PADG_03136 | 0.0 | 100% | 97% |
Mitochondrial | PAAG_05350 | 0.0 | 97% | 100% |
Mitochondrial acetonate hydratase | PAAG_00845 | 0.0 | 100% | 99% |
NADP-especific glutamate dehydrogenase | PADG_04516 | 0.0 | 98% | 100% |
2-nitropropane dioxygenase | PAAG_06693 | 0.0 | 98% | 100% |
Osmosensor | PAAG_04025 | 0.0 | 100% | 97% |
Oligosaccharide transferase | PAAG_04719 | 0.0 | 98% | 100% |
Oxoisovalerate dehydrogenase beta subunit | PAAG_01194.2 | 0.0 | 99% | 100% |
Oxoisovalerate dehydrogenase alpha subunit | PAAG_01310 | 0.0 | 98% | 100% |
Oxirreductase | PADG_06082 | 0.0 | 90% | 100% |
Plasma ATPase | PAAG_08082 | 0.0 | 99% | 100% |
Phosphatase ser/thre | PADG_03544 | 0.0 | 100% | 100% |
Plasma ATPase | PAAG_08082 | 0.0 | 99% | 100% |
Perixosomal catalase | PADG_01943 | 0.0 | 100% | 95% |
Perixosomal catalase | PADG_00686 | 0.0 | 100% | 96% |
Perixosomal hydratase | PADG_08651 | 0.0 | 100% | 97% |
Pyruvate dehydrogenase component E1 beta E1 | PAAG_01534 | 0.0 | 98% | 100% |
Pyruvate dehydrogenase | PADG_00246 | 0.0 | 100% | 98% |
Shock protein SSVB1 | PAAG_07775 | 0.0 | 98% | 100% |
Serine 3 dehydrogenase | PAAG_02354 | 0.0 | 97% | 100% |
Serine hydroxymethyltransferase | PAAG_07412 | 0.0 | 99% | 100% |
Sorbitol SOU2 | PAAG_04184 | 0.0 | 98% | 100% |
Succinate dehydrogenase | PADG_06494 | 0.0 | 97% | 100% |
Succinate dehydrogenase | PADG_08013 | 0.0 | 100% | 98% |
Succinyl CoA ligase | PADG_02260 | 0.0 | 99% | 100% |
Shock protein 90 | PADG_02785 | 0.0 | 100% | 98% |
Shock protein 98 | PADG_00765 | 0.0 | 100% | 99% |
Shock protein SSVB1 | PAAG_07775 | 0.0 | 98% | 100% |
Transferrin | PADG_00686 | 0.0 | 100% | 98% |
Transketolase | PADG_00246 | 0.0 | 100% | 98% |
Tetracycline transporter | PAAG_07990 | 0.0 | 97% | 100% |
Thioredoxin | PADG_03161 | 0.0 | 100% | 97% |
Thioredoxin | PADG_01551 | 0.0 | 100% | 97% |
Urease | PADG_03874 | 0.0 | 100% | 97% |
Urease | PADG_00954 | 0.0 | 100% | 97% |
Xaa-Pro aminopeptidase | PAAG_07500 | 0.0 | 100% | 100% |
Pep_H1 MSAFSRMTASLGFSK (PADG_06178) | |||
---|---|---|---|
Peptide | Allele | Method Used | Percentage Rank (%) |
MSAFSRMTASLGFSK | HLA-DRB1 * 07:01 | Consensus (comb.lib./smm/nn) | 1.11 |
HLA-DRB3 * 02:02 | NetMHCIIpan | 2.37 | |
HLA-DRB5 * 01:01 | Consensus (smm/nn/sturniolo) | 2.92 | |
HLA-DRB1 * 15:01 | Consensus (smm/nn/sturniolo) | 14.19 | |
HLA-DRB1 * 03:01 | Consensus (smm/nn/sturniolo) | 14.96 | |
HLA-DRB3 * 01:01 | Consensus (comb.lib./smm/nn) | 20.72 | |
HLA-DRB4 * 01:01 | Consensus (comb.lib./smm/nn) | 32.06 | |
Pep_SQ2FDVFYYLLTSASTPA (>ABV31248.1) | |||
FDVFYYLLTSASTPA | HLA-DRB1 * 07:01 | Consensus (comb.lib./smm/nn) | 1.67 |
HLA-DRB3 * 01:01 | Consensus (smm/nn/sturniolo) | 3.95 | |
HLA-DRB1 * 15:01 | Consensus (smm/nn/sturniolo) | 3.58 | |
HLA-DRB3 * 02:02 | NetMHCIIpan | 4.02 | |
HLA-DRB5 * 01:01 | Consensus (smm/nn/sturniolo) | 4.34 | |
HLA-DRB3 * 01:01 | Consensus (comb.lib./smm/nn) | 40.25 | |
HLA-DRB4 * 01:01 | Consensus (comb.lib./smm/nn) | 42.57 | |
Pep_PH3RAYALLFSKLGAAVV (PADG_08651) | |||
HLA-DRB5 * 01:01 | Consensus (smm/nn/sturniolo) | 3.19 | |
HLA-DRB1 * 15:01 | Consensus (smm/nn/sturniolo) | 3.37 | |
HLA-DRB1 * 07:01 | Consensus (smm/nn/sturniolo) | 4.79 | |
HLA-DRB3 * 02:02 | NetMHCIIpan | 11.93 | |
HLA-DRB1 * 03:01 | Consensus (smm/nn/sturniolo) | 17.05 | |
HLA-DRB4 * 01:01 | Consensus (comb.lib./smm/nn) | 23.65 | |
HLA-DRB3 * 01:01 | Consensus (comb.lib./smm/nn) | 32.36 | |
Pep_ SQ4ERVSIIANPAVASLY (PAAG_08203) | |||
HLA-DRB3 * 02:02 | NetMHCIIpan | 0.01 | |
HLA-DRB1 * 15:01 | Consensus (smm/nn/sturniolo) | 2.60 | |
HLA-DRB1 * 03:01 | Consensus (smm/nn/sturniolo) | 11.00 | |
HLA-DRB1 * 07:01 | Consensus (comb.lib./smm/nn) | 15.00 | |
HLA-DRB4 * 01:01 | Consensus (comb.lib./smm/nn) | 19.00 | |
HLA-DRB3 * 01:01 | Consensus (comb.lib./smm/nn) | 7.70 | |
HLA-DRB5 * 01:01 | Consensus (comb.lib./smm/nn) | 43.00 |
Epitope | Method | IFN-ɤ | Score IFN-ɤ | Score Other Cytokines | Score Random IFN-ɤ |
---|---|---|---|---|---|
Epi 1—MSAFSRMTASLGFSK | Scan/MSV | Negative | −0.14 | 0.49 | 0.81 |
Epi 2—FDVFYYLLTSASTPA | Scan/MSV | Negative | −0.56 | 0.44 | −0.00 |
Epi 3—RAYALLFSKLGAAVV | Scan/MSV | Positive | −0.16 | 0.61 | 0.00 |
Epi 4—ERVSIIANPAVASLY | Scan/MSV | Positive | 0.15 | 0.51 | −0.38 |
ESAT-6—QWNFAGIEAAASAIQ | San/MSV | Positive | 0.30 | 0.88 | 0.63 |
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Rosa, S.B.A.; Csordas, B.G.; do Valle Leone de Oliveira, S.M.; Ribeiro dos Santos, A.; Paniago, A.M.M.; Venturini, J. Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy. J. Fungi 2020, 6, 379. https://doi.org/10.3390/jof6040379
Rosa SBA, Csordas BG, do Valle Leone de Oliveira SM, Ribeiro dos Santos A, Paniago AMM, Venturini J. Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy. Journal of Fungi. 2020; 6(4):379. https://doi.org/10.3390/jof6040379
Chicago/Turabian StyleRosa, Sarah Brena Aparecida, Bárbara Guimarães Csordas, Sandra Maria do Valle Leone de Oliveira, Amanda Ribeiro dos Santos, Anamaria Mello Miranda Paniago, and James Venturini. 2020. "Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy" Journal of Fungi 6, no. 4: 379. https://doi.org/10.3390/jof6040379