Role of acuK in Control of Iron Acquisition and Gluconeogenesis in Talaromyces marneffei
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Culture Conditions
2.2. Talaromyces marneffei acuK Gene Deletion and Complementation
2.3. Macroscopic and Microscopic Morphology Examination
2.4. Germination Assay
2.5. Determination of Growth in Iron-Depletion and Iron-Repletion Conditions
2.6. Determination of Growth on Medium Containing Gluconeogenic Substrates
2.7. Real-Time Reverse Transcription-Polymerase Chain Reaction (Real-Time RT-PCR)
2.8. Chrome Azurol S (CAS) Assay
2.9. Macrophage Infection for Phagocytosis and Killing Assay
2.10. Statistical Analysis
3. Results
3.1. Mutation of acuK Reduced Growth and Conidial Germination
3.2. ΔacuK Showed Abnormal Hyphae with Double Nuclei
3.3. AcuK Plays a Role in Iron Assimilation
3.4. AcuK Is Required for Gluconeogenesis
3.5. AcuK Mutation Increased Percentages of Phagocytosis and Killing by a Human Macrophage
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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T. marneffei Strain | % Phagocytosis | Phagocytic Index (PI) | % Killing |
---|---|---|---|
Wild type | 40 ± 0.04 | 1.43 ± 0.01 | 66.12 ± 1.86 |
ΔacuK | 41 ± 0.12 | 3.39 ± 0.00 | 73.66 ± 2.80 |
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Amsri, A.; Jeenkeawpieam, J.; Sukantamala, P.; Pongpom, M. Role of acuK in Control of Iron Acquisition and Gluconeogenesis in Talaromyces marneffei. J. Fungi 2021, 7, 798. https://doi.org/10.3390/jof7100798
Amsri A, Jeenkeawpieam J, Sukantamala P, Pongpom M. Role of acuK in Control of Iron Acquisition and Gluconeogenesis in Talaromyces marneffei. Journal of Fungi. 2021; 7(10):798. https://doi.org/10.3390/jof7100798
Chicago/Turabian StyleAmsri, Artid, Juthatip Jeenkeawpieam, Panwarit Sukantamala, and Monsicha Pongpom. 2021. "Role of acuK in Control of Iron Acquisition and Gluconeogenesis in Talaromyces marneffei" Journal of Fungi 7, no. 10: 798. https://doi.org/10.3390/jof7100798