Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant and Fungal Materials
2.2. L. bicolor–P. trichocarpa–B. dothidea Coculturing in Two Sandwich Culture Systems
2.3. Estimation of Peroxidase (POD) and L-phenylalanine Ammonia-Lyase (PAL)
2.4. Content of Malondialdehyde (MDA)
2.5. RNA Extraction, Transcriptome Sequencing, and Bioinformatics Analysis
2.6. The Quantitative Real-Time PCR (qRT-PCR)
2.7. Statistical Analysis
3. Results
3.1. Enzyme Activity Analysis
3.2. Analysis of Differentially Expressed Genes between Different Treatments
3.3. DEGs Enrichment Analysis
3.4. Analysis of Gene Expression Patterns Related to Signal Transduction Induced by L. bicolor
3.5. Analysis of the Expression Pattern of Disease Resistance-Related and Antioxidant-Related DEGs Induced by L. bicolor
3.6. The qRT-PCR Verification
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | POD | PAL | MDA |
---|---|---|---|
NN | 1054.02 ± 325.66 c | 5678.41 ± 1428.19 c | 14.30 ± 3.90 d |
EN | 3516.56 ± 484.89 ab | 16600.94 ± 779.80 ab | 35.49 ± 5.53 c |
NB | 2330.89 ± 465.16 bc | 10399.50 ± 2842.16 bc | 128.66 ± 11.48 a |
EB | 5252.68 ± 1370.74 a | 23236.47 ± 5403.70 a | 60.68 ± 8.61 b |
L. bicolor | ** | ** | ** |
B. dothidea | * | * | ** |
L. bicolor & B. dothidea | ns | ns | ** |
Comparisons (Control/Treatment) | All DEGs | Up Regulated DEGs | Down Regulated DEGs |
---|---|---|---|
NN/EN | 747 | 297 | 450 |
NN/NB | 948 | 734 | 214 |
EN/EB | 1420 | 736 | 684 |
NB/EB | 1138 | 288 | 850 |
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Dong, F.; Wang, Y.; Tang, M. Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress. J. Fungi 2021, 7, 1024. https://doi.org/10.3390/jof7121024
Dong F, Wang Y, Tang M. Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress. Journal of Fungi. 2021; 7(12):1024. https://doi.org/10.3390/jof7121024
Chicago/Turabian StyleDong, Fengxin, Yihan Wang, and Ming Tang. 2021. "Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress" Journal of Fungi 7, no. 12: 1024. https://doi.org/10.3390/jof7121024