Transcription Activator Swi6 Interacts with Mbp1 in MluI Cell Cycle Box-Binding Complex and Regulates Hyphal Differentiation and Virulence in Beauveria bassiana
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Bioinformatic Identification and Functional Analyses of BbSwi6
2.3. Sub-Cellular Localization of BbMbp1 and BbSwi6
2.4. Quantification of Blastospore Production
2.5. Assay for Conidial Production
2.6. Fungal Growth
2.7. Insect Bioassay
2.8. Yeast Two Hybrid (Y2H) Assays
2.9. Analyzing the BbSwi6-Mediated Transcriptome during Sporulation
2.10. Data Analyses
3. Results
3.1. Bioinformatic Analysis of BbSwi6 and Generation of Its Gene Disruption and Complementation Strains
3.2. BbSwi6 Interacts with BbMbp1 in Nucleus
3.3. BbSwi6 Significantly Contributes to Vegetative Growth onNutrients
3.4. BbSwi6 Is Required for Asexual Sporulation
3.5. Disruption of BbSwi6Significantly Affected Fungal Pathogenicity
3.6. BbSwi6 Mediates the Genome-Wide Expression during Fungal Differentiation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ding, J.-L.; Hou, J.; Li, X.-H.; Feng, M.-G.; Ying, S.-H. Transcription Activator Swi6 Interacts with Mbp1 in MluI Cell Cycle Box-Binding Complex and Regulates Hyphal Differentiation and Virulence in Beauveria bassiana. J. Fungi 2021, 7, 411. https://doi.org/10.3390/jof7060411
Ding J-L, Hou J, Li X-H, Feng M-G, Ying S-H. Transcription Activator Swi6 Interacts with Mbp1 in MluI Cell Cycle Box-Binding Complex and Regulates Hyphal Differentiation and Virulence in Beauveria bassiana. Journal of Fungi. 2021; 7(6):411. https://doi.org/10.3390/jof7060411
Chicago/Turabian StyleDing, Jin-Li, Jia Hou, Xiu-Hui Li, Ming-Guang Feng, and Sheng-Hua Ying. 2021. "Transcription Activator Swi6 Interacts with Mbp1 in MluI Cell Cycle Box-Binding Complex and Regulates Hyphal Differentiation and Virulence in Beauveria bassiana" Journal of Fungi 7, no. 6: 411. https://doi.org/10.3390/jof7060411