Histidine Kinase Sln1 and cAMP/PKA Signaling Pathways Antagonistically Regulate Sporisorium scitamineum Mating and Virulence via Transcription Factor Prf1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Construction of Strains
2.3. Nucleic Acid Related Manipulation
2.4. Sporidia Staining and Epifluorescence Microscopy
2.5. SsHog1 Phosphorylation Assays
2.6. Yeast Two-Hybrid (Y2H) Assays
2.7. Assessment of Pathogenicity and Relative Fungal Biomass
2.8. Statistical Analysis
3. Results
3.1. Identification of SsSln1 Protein in S. scitamineum
3.2. Deletion of SsSLN1 Gene Promotes Mating and Pathogenicity of S. scitamineum
3.3. SsSln1 Negatively Regulates Phosphorylation and Nuclear Localization of SsHog1
3.4. Phosphorylation of SsHog1 Is Necessary for Mating and Virulence of S. scitamineum
3.5. Phosphorylation Level of SsHog1 Positively Mediates Mating and Virulence of S. scitamineum
3.6. SsSln1 Negatively Regulates the Transcription of SsPRF1 by SsHog1 Phosphorylation, but Not through the cAMP/PKA Signaling Pathway
3.7. Cross-Talk between the SsSln1 and cAMP/PKA Pathways Antagonistically Regulates Mating and Virulence by SsPRF1 Transcription
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strains | Accession Number for Protein | Resistance Marker and Strain Background | Reference or Source |
---|---|---|---|
MAT-1 | a1, b1 | [2] | |
MAT-2 | a2, b2 | [2] | |
ss1sln1Δ | CDU22142.1 | Hygromycin, MAT-1 | This study |
ss2sln1Δ | CDU22142.1 | Hygromycin, MAT-2 | This study |
Ss1SLN1-COM | CDU22142.1 | Zeocin, ss1sln1Δ | This study |
Ss2SLN1-COM | CDU22142.1 | Zeocin, ss2sln1Δ | This study |
ss1atf1Δ | CDU21933.1 | Hygromycin, MAT-1 | This study |
ss2atf1Δ | CDU21933.1 | Hygromycin, MAT-2 | This study |
Ss1ATF1-COM | CDU21933.1 | Zeocin, ss1atf1Δ | This study |
Ss2ATF1-COM | CDU21933.1 | Zeocin, ss2atf1Δ | This study |
ss1hog1Δ | CDU23149.1 | Hygromycin, MAT-1 | [41] |
Ss1PTP1-OE | CDU22358.1 | Zeocin, MAT-1 | This study |
Ss2PTP1-OE | CDU22358.1 | Zeocin, MAT-2 | This study |
Ss1PTP2-OE | CDU23562.1 | Zeocin, MAT-1 | This study |
Ss2PTP2-OE | CDU23562.1 | Zeocin, MAT-2 | This study |
Ss1PRF1-OE(hog1Δ) | CDU22680.1 | Zeocin, ss1hog1Δ | This study |
Ss1Hog1:RFP | CDU23149.1 | Hygromycin, MAT-1 | This study |
Ss1Hog1:eGFP(sln1Δ) | CDU23149.1 | Zeocin, ss1sln1Δ | This study |
Ss1Atf1:eGFP | CDU21933.1 | Zeocin, MAT-1 | This study |
Ss1Atf1:eGFP(hog1Δ) | CDU21933.1 | Zeocin, ss1hog1Δ | This study |
ss1sln1Δgpa3Δ | CDU22378.1 | Zeocin, ss1sln1Δ | This study |
ss1sln1Δuac1Δ | CDU25762.1 | Zeocin, ss1sln1Δ | This study |
ss1sln1Δadr1Δ | CDU22361.1 | Zeocin, ss1sln1Δ | This study |
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Cai, E.; Sun, S.; Deng, Y.; Huang, P.; Sun, X.; Wang, Y.; Chang, C.; Jiang, Z. Histidine Kinase Sln1 and cAMP/PKA Signaling Pathways Antagonistically Regulate Sporisorium scitamineum Mating and Virulence via Transcription Factor Prf1. J. Fungi 2021, 7, 610. https://doi.org/10.3390/jof7080610
Cai E, Sun S, Deng Y, Huang P, Sun X, Wang Y, Chang C, Jiang Z. Histidine Kinase Sln1 and cAMP/PKA Signaling Pathways Antagonistically Regulate Sporisorium scitamineum Mating and Virulence via Transcription Factor Prf1. Journal of Fungi. 2021; 7(8):610. https://doi.org/10.3390/jof7080610
Chicago/Turabian StyleCai, Enping, Shuquan Sun, Yizhen Deng, Peishen Huang, Xian Sun, Yuting Wang, Changqing Chang, and Zide Jiang. 2021. "Histidine Kinase Sln1 and cAMP/PKA Signaling Pathways Antagonistically Regulate Sporisorium scitamineum Mating and Virulence via Transcription Factor Prf1" Journal of Fungi 7, no. 8: 610. https://doi.org/10.3390/jof7080610