DNA Methyltransferases Regulate Pathogenicity of Botrytis cinerea to Horticultural Crops
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Phylogenetic Analysis
2.3. 5-Azacytidine Treatment
2.4. Construction of Knockout Mutants
2.5. Expression Analysis
2.6. Virulence Assay
2.7. ROS Detection
2.8. Quantification of Global DNA Methylation
2.9. Whole-Genome Bisulfite Sequencing
2.10. Statistical Analysis of Data
3. Results
3.1. DNA Methylation Is Involved in the Infection Process of B. cinerea
3.2. DNA Methylation Is Involved in the Development of B. cinerea
3.3. Virulence Assay of DNA MTase Mutants on Fruit Hosts
3.4. Verification of the Synergistic Effect of Bcdim2 and Bcrid2 on Leaf Hosts
3.5. DNA MTases Ais Attenuatedre Involved in the Development of B. cinerea
3.6. The Oxidative Tolerance of ΔBcdim2rid2 Is Attenuated
3.7. Expression of Pathogenic Genes in ΔBcdim2rid2 Is Inhibited
3.8. ΔBcdim2rid2 Induces the Resistant of Host
3.9. DNA MTases Affect Genomic DNA Methylation of B. cinerea
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, Z.; He, C.; Chen, Y.; Li, B.; Tian, S. DNA Methyltransferases Regulate Pathogenicity of Botrytis cinerea to Horticultural Crops. J. Fungi 2021, 7, 659. https://doi.org/10.3390/jof7080659
Zhang Z, He C, Chen Y, Li B, Tian S. DNA Methyltransferases Regulate Pathogenicity of Botrytis cinerea to Horticultural Crops. Journal of Fungi. 2021; 7(8):659. https://doi.org/10.3390/jof7080659
Chicago/Turabian StyleZhang, Zhanquan, Chang He, Yong Chen, Boqiang Li, and Shiping Tian. 2021. "DNA Methyltransferases Regulate Pathogenicity of Botrytis cinerea to Horticultural Crops" Journal of Fungi 7, no. 8: 659. https://doi.org/10.3390/jof7080659