Mating-Type Genes Play an Important Role in Fruiting Body Development in Morchella sextelata
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Fruiting Body Cultivation and Sample Collection
2.3. Morphological Observation and Measurement
2.4. Construction of RNAi Plasmids for mat1-2-1
2.5. Agrobacterium-Mediated Transformation of M. sextelata
2.6. DNA Extraction and PCR
2.7. RNA Extraction and Reverse-Transcription (RT)-PCR
3. Results
3.1. Mating Type Detection at the Different Stages
3.2. Different Mating Types Generate Divergent Physiological Structures of Fruiting Bodies
3.3. Mating Type Influences Ascus and Ascospore Development
3.4. Transcription Levels of Mating-Type Genes and Ascus Development-Related Genes in Pileus
3.5. mat1-2-1 Regulated Ascus Development-Related Genes at the Mycelial Stage
4. Discussion
4.1. Is It Mating-Type Switching?
4.2. Mating-Type Genes Regulate Fruiting Body Development
4.3. A Conservative Mechanism of Controlling Target Gene Expression by Mating-Type Genes
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Sequence (5′ to 3′) |
---|---|
mat1-1-1-QL | GCCTTCTGAGT CCGTTAT |
mat1-1-1-QR | ATGTGAGCGTCCCTTGA |
mat1-2-1-QL | GCCGTGACCCTCCTTCT |
mat1-2-1-QR | TCGTTGTTCCCAATCCC |
hyg-F | CTGTCGAGAAGTTTCTGATCG |
hyg-R | CTGATAGAGTTGGTCAAGACC |
mat1-1-1-RT-F | TGTCTTCGTAACGCCACT |
mat1-1-1-RT-R | TAGCCCAACCCTTCCA |
mat1-1-10-RT-F | TCGGAGGATGTTGGGTT |
mat1-1-10-RT-R | ACGCTGCTTGAAGTATGGA |
mat1-1-11-RT-F | GTGAAGCCATCAACAA |
mat1-1-11-RT-R | GAGCCTTTCGTCAATAC |
mat1-2-1-RT-F | CCAGGGAAAGAAAGTG |
mat1-2-1-RT-R | CATAGGACGAGGAACAT |
372751-RT-F | TGGACCGATTAGGGAG |
372751-RT-R | TGTTGTTGCGGATGAC |
484741-RT-F | ACATTGTCACCACCCTC |
484741-RT-R | CCAAACTACTCGCCTTC |
504283-RT-F | TAAGGACGGCTATGTAA |
504283-RT-R | TCCGCAACTAGACCAC |
481101-RT-F | ACCTTACCCAACCTGA |
481101-RT-R | CTATCGTCCGCATTTA |
533191-RT-F | TCAACTACCACCTCACCC |
533191-RT-R | TCCCTCGCCAAAGAAC |
479298-RT-F | AACACTCTTGACCTCCAC |
479298-RT-R | CATCAGTAACCGCCTC |
543093-RT-F | GAACACTTACACGCCTAC |
543093-RT-R | CATCTCAGCCATCTCG |
533505-RT-F | ATTCCACCAAACAACG |
533505-RT-R | CCAACATAAACACCACC |
517289-RT-F | TGTCACCTCCATCTGTC |
517289-RT-R | GTATTCGGTCCGTCAT |
HindIII-GPD-F1 | TGCCAAGCTTCGGGAGGTACGGGGAGAATA |
SbfI-GPD-B1 | CAACCTGCAGGTTTGACTATTTAGTG |
SpeI-35S-F3 | GAAACTAGTGTTTGATCGAGACCTAATACAGCC |
SbfI-35S-B3 | ATTCACTAAATAGTCAAACCTGCAGGGGTGATGTCTGCTCAAGC |
Part-mat1-2-1-F | TTGAGCAGACATCACCCCTGCAGGCTTCGGCCAGAACAGATG |
Part-mat1-2-1-R | ATTCACTAAATAGTCAAACCTGCAGGGCATTCATAGGACGAG |
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Liu, Q.; Qu, S.; He, G.; Wei, J.; Dong, C. Mating-Type Genes Play an Important Role in Fruiting Body Development in Morchella sextelata. J. Fungi 2022, 8, 564. https://doi.org/10.3390/jof8060564
Liu Q, Qu S, He G, Wei J, Dong C. Mating-Type Genes Play an Important Role in Fruiting Body Development in Morchella sextelata. Journal of Fungi. 2022; 8(6):564. https://doi.org/10.3390/jof8060564
Chicago/Turabian StyleLiu, Qizheng, Shan Qu, Guoqiang He, Jinkang Wei, and Caihong Dong. 2022. "Mating-Type Genes Play an Important Role in Fruiting Body Development in Morchella sextelata" Journal of Fungi 8, no. 6: 564. https://doi.org/10.3390/jof8060564