Identification of miRNAs Involved in Maize-Induced Systemic Resistance Primed by Trichoderma harzianum T28 against Cochliobolus heterostrophus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Microbial Treatment
2.2. Construction and Analysis of Small RNA Libraries and Transcriptome Libraries
2.3. qRT-PCR Validation
2.4. Statistical Analysis
3. Results
3.1. Maize Resistance to SCLB Systemically Induced by T. harzianum T28
3.2. Small RNA Profile Analysis in Maize B73 and B73 + T28 after C. heterostrophus Infection
3.3. Differentially Expressed miRNAs Responsive to ISR Primed by T. harzianum
3.4. Global mRNA Expression Profiles in Maize B73 and B73 + T28 after C. heterostrophus Infection
3.5. Identification of Differentially Expressed Genes (DEGs) in Maize B73 and B73 + T28 after C. heterostrophus Infection
3.6. Combined Analysis of DEMs and DEGs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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miRNA | Foldchange (miRNA) (B73_T28/B73) | Target Gene | Foldchange (Gene) (B73_T28/B73) | Target_Start | Target_End | Score | Description |
---|---|---|---|---|---|---|---|
zma-miR390b-5p | 0.63 | Zm00001d002835 | 1.99 | 657 | 677 | 1 | leucine-rich repeat transmembrane protein kinase family protein |
zma-miR169j-5p | 0.35 | Zm00001d018255 | 1.66 | 1014 | 1034 | 2 | nuclear transcription factor Y subunit A-3 isoform X5 |
zma-miR169j-5p | 0.35 | Zm00001d027874 | 2.18 | 1321 | 1341 | 2 | nuclear transcription factor Y subunit A |
zma-miR408b-3p | 0.54 | Zm00001d031257 | 1.55 | 850 | 870 | 2 | Cupredoxin superfamily protein |
zma-miR408b-3p | 0.54 | Zm00001d002434 | 2.62 | 1930 | 1950 | 3 | alternative oxidase3 |
zma-miR408b-5p | 0.66 | Zm00001d040364 | 3.05 | 163 | 183 | 3.5 | peroxidase |
zma-miR395p-3p | 0.59 | Zm00001d002435 | 2.38 | 78 | 98 | 3 | AOX1B, Ubiquinol oxidase |
zma-miR395p-3p | 0.59 | Zm00001d032518 | 1.78 | 108 | 127 | 2.5 | Pentatricopeptide repeat-containing protein |
zma-miR395a-5p | 0.06 | Zm00001d025524 | 1.90 | 401 | 423 | 3.5 | lipoxygenase7 |
zma-miR399d-3p, zma-miR399h-3p, zma-miR399b-3p, zma-miR399j-3p | 4.01 | Zm00001d019111 | 0.00 | 140 | 160 | 3.5 | serine palmitoyltransferase |
zma-miR399c-5p | Inf | Zm00001d044197 | 0.43 | 1843 | 1863 | 3 | pentatricopeptide repeat protein 203 |
zma-miR399c-5p | Inf | Zm00001d033232 | 0.21 | 259 | 279 | 3.5 | Pollen Ole e 1 allergen and extensin family protein |
zma-miR1432-5p | 1.58 | Zm00001d046838 | 0.45 | 2562 | 2582 | 4 | Putative receptor-like protein kinase family protein |
zma-miR1432-5p | 1.58 | Zm00001d007821 | 0.00 | 706 | 726 | 4 | Predicted CLIP-associated protein |
zma-miRn5231-5p | 0.01 | Zm00001d014628 | 1.58 | 505 | 528 | 4.5 | Homeobox-transcription factor 42 |
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Wang, S.; Wang, X.; Chen, J. Identification of miRNAs Involved in Maize-Induced Systemic Resistance Primed by Trichoderma harzianum T28 against Cochliobolus heterostrophus. J. Fungi 2023, 9, 278. https://doi.org/10.3390/jof9020278
Wang S, Wang X, Chen J. Identification of miRNAs Involved in Maize-Induced Systemic Resistance Primed by Trichoderma harzianum T28 against Cochliobolus heterostrophus. Journal of Fungi. 2023; 9(2):278. https://doi.org/10.3390/jof9020278
Chicago/Turabian StyleWang, Shaoqing, Xinhua Wang, and Jie Chen. 2023. "Identification of miRNAs Involved in Maize-Induced Systemic Resistance Primed by Trichoderma harzianum T28 against Cochliobolus heterostrophus" Journal of Fungi 9, no. 2: 278. https://doi.org/10.3390/jof9020278