The AMP-Activated Protein Kinase (AMPK) Positively Regulates Lysine Biosynthesis Induced by Citric Acid in Flammulina filiformis
Abstract
:1. Introduction
2. Experimental Procedures
2.1. Strains and Culture Conditions
2.2. Mycelium Treatment Conditions
2.3. Fruiting Body Cultivation of F. filiformis Induced by Citric Acid
2.4. Measurement of Mycelium Biomass and Growth Rate
2.5. Determination of Lysine Content
2.6. Optimization by Response Surface Methodology
2.7. RNA Extraction and Gene Expression Analysis
2.8. AMPK-Silencing Strain Construction
2.9. Western Blot for AMPK Total Protein and Phosphorylation Levels
2.10. Statistical Analysis
3. Results
3.1. Citric Acid Induction Increased the Biomass, Growth Rate, and Lysine Content of F. filiformis
3.2. Response Surface Methodology to Optimize Citric Acid Induction Conditions
3.3. Citric Acid Induction Changes the Lysine Content of F. filiformis Fruiting Bodies
3.4. Changes in Lysine Content, Gene Transcription Level, and Key Regulators under Optimal Citric Acid Induction Conditions
3.5. Changes in the Phosphorylation and Lysine Content of Ampk-Silenced Strains under Optimal Induction by Citric Acid
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Fan, H.; Ge, F.; Wu, T.; Liu, Y.; Tian, L.; Liu, Y.; Xiang, T.; Yu, H.; Shi, L.; He, Q.; et al. The AMP-Activated Protein Kinase (AMPK) Positively Regulates Lysine Biosynthesis Induced by Citric Acid in Flammulina filiformis. J. Fungi 2023, 9, 340. https://doi.org/10.3390/jof9030340
Fan H, Ge F, Wu T, Liu Y, Tian L, Liu Y, Xiang T, Yu H, Shi L, He Q, et al. The AMP-Activated Protein Kinase (AMPK) Positively Regulates Lysine Biosynthesis Induced by Citric Acid in Flammulina filiformis. Journal of Fungi. 2023; 9(3):340. https://doi.org/10.3390/jof9030340
Chicago/Turabian StyleFan, Hao, Feng Ge, Tao Wu, Yongzhi Liu, Li Tian, Yueqian Liu, Taobo Xiang, Hanshou Yu, Liang Shi, Qin He, and et al. 2023. "The AMP-Activated Protein Kinase (AMPK) Positively Regulates Lysine Biosynthesis Induced by Citric Acid in Flammulina filiformis" Journal of Fungi 9, no. 3: 340. https://doi.org/10.3390/jof9030340