Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2

Li, Ruiying; Zheng, Pu; Sun, Xingyun; Dong, Wenhua; Shen, Ziqiang; Chen, Pengcheng; Wu, Dan

doi:10.3390/jof9050522

Open AccessArticle

Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2

by

Ruiying Li

,

Pu Zheng

^*,

Xingyun Sun

,

Wenhua Dong

,

Ziqiang Shen

,

Pengcheng Chen

and

Dan Wu

The Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Ministry of Education, Wuxi 214122, China

^*

Author to whom correspondence should be addressed.

J. Fungi 2023, 9(5), 522; https://doi.org/10.3390/jof9050522

Submission received: 15 March 2023 / Revised: 19 April 2023 / Accepted: 27 April 2023 / Published: 28 April 2023

(This article belongs to the Special Issue Genomics Analysis of Fungi)

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Abstract

Lasiodiplodia sp. is a typical opportunistic plant pathogen, which can also be classified as an endophytic fungus. In this study, the genome of a jasmonic-acid-producing Lasiodiplodia iranensis DWH-2 was sequenced and analyzed to understand its application value. The results showed that the L. iranensis DWH-2 genome was 43.01 Mb in size with a GC content of 54.82%. A total of 11,224 coding genes were predicted, among which 4776 genes were annotated based on Gene Ontology. Furthermore, the core genes involved in the pathogenicity of the genus Lasiodiplodia were determined for the first time based on pathogen–host interactions. Eight Carbohydrate-Active enzymes (CAZymes) genes related to 1,3-β-glucan synthesis were annotated based on the CAZy database and three relatively complete known biosynthetic gene clusters were identified based on the Antibiotics and Secondary Metabolites Analysis Shell database, which were associated with the synthesis of 1,3,6,8-tetrahydroxynaphthalene, dimethylcoprogen, and (R)-melanin. Moreover, eight genes associated with jasmonic acid synthesis were detected in pathways related to lipid metabolism. These findings fill the gap in the genomic data of high jasmonate-producing strains.

Keywords: Lasiodiplodia; genome sequencing; secondary metabolite; 1,3-β-glucan; jasmonic acid

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MDPI and ACS Style

Li, R.; Zheng, P.; Sun, X.; Dong, W.; Shen, Z.; Chen, P.; Wu, D. Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2. J. Fungi 2023, 9, 522. https://doi.org/10.3390/jof9050522

AMA Style

Li R, Zheng P, Sun X, Dong W, Shen Z, Chen P, Wu D. Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2. Journal of Fungi. 2023; 9(5):522. https://doi.org/10.3390/jof9050522

Chicago/Turabian Style

Li, Ruiying, Pu Zheng, Xingyun Sun, Wenhua Dong, Ziqiang Shen, Pengcheng Chen, and Dan Wu. 2023. "Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2" Journal of Fungi 9, no. 5: 522. https://doi.org/10.3390/jof9050522

APA Style

Li, R., Zheng, P., Sun, X., Dong, W., Shen, Z., Chen, P., & Wu, D. (2023). Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2. Journal of Fungi, 9(5), 522. https://doi.org/10.3390/jof9050522

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Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2

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