Molecular Real-Time PCR Monitoring of Onion Fusarium Basal Rot Chemical Control
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Species’ Source and Growth Conditions
2.2. Brief Description of the Semi-Field Trial
2.3. Evaluation of Disease Severity under Chemical Control
2.4. Real-Time PCR Molecular Evaluation
2.5. Statistical Analysis
3. Results
3.1. Evaluation of Disease Severity
3.2. Real-Time PCR Molecular Evaluation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pairs | Primer | Sequence 1 | Uses | Amplification | References |
---|---|---|---|---|---|
Pair 1 | Fus-for Fus-rev | 5′-CGACCACTGTGAGTACTACCATC-3′ 5′-ACCGGTCTGTCAAGCTATGT-3′ | Target gene | Fusarium spp.-specific fragment, qPCR cycling—27 or above | This work |
Pair 3 | COX-F COX-R | 5′-GTATGCCACGTCGCATTCCAGA-3′ 5′-CAACTACGGATATATAAGRRCCRRAACTG-3′ | Control | Cytochrome C oxidase (COX) gene product, qPCR cycling—27 or below | [27,28] |
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Dimant, E.; Degani, O. Molecular Real-Time PCR Monitoring of Onion Fusarium Basal Rot Chemical Control. J. Fungi 2023, 9, 809. https://doi.org/10.3390/jof9080809
Dimant E, Degani O. Molecular Real-Time PCR Monitoring of Onion Fusarium Basal Rot Chemical Control. Journal of Fungi. 2023; 9(8):809. https://doi.org/10.3390/jof9080809
Chicago/Turabian StyleDimant, Elhanan, and Ofir Degani. 2023. "Molecular Real-Time PCR Monitoring of Onion Fusarium Basal Rot Chemical Control" Journal of Fungi 9, no. 8: 809. https://doi.org/10.3390/jof9080809