New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis of Hydrogels and FT-IR Data
2.2. Hydrogel Morphology
2.3. Swelling Properties
2.4. Bioadhesive Properties
2.5. In Vitro Drug Release
2.6. In Vitro Cytotoxicity Studies
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Synthesis of Hydrogels
4.3. Hydrogel Characterisation
4.3.1. Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM)
4.3.2. Swelling Properties
4.3.3. Bioadhesive Characteristics
4.3.4. Drug-Loading and Drug-Release Studies
4.3.5. In vitro Cytotoxicity Studies
Hydrogel Preparation for Cytotoxicity Testing
Cell Morphology Evaluation by Fluorescence Microscopy
4.3.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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DexMa-CsMa (µm) | DexMa-CsMa-Aam (µm) | DexMa-CsMa-BisAam (µm) | DexMa-GelMa-BisAam (µm) | DexMa-XMa-BisAam (µm) | |||||
---|---|---|---|---|---|---|---|---|---|
Min | Max | Min | Max | Min | Max | Min | Max | Min | Max |
27.75 ± 4.17 | 80.95 ± 6.43 | 47.15 ± 3.49 | 143.10 ± 9.12 | 52.07 ± 6.88 | 131.46 ± 2.09 | 30.68 ± 5.04 | 46.15 ± 4.66 | 41.95 ± 11.17 | 88.96 ± 6.09 |
Hydrogel | Correlation Coefficient (r2) | Release Rate Constant, k (h−n) | Release Exponent, n | |
---|---|---|---|---|
Higuchi | Korsmeyer–Peppas | |||
DexMa-CsMa | 0.9812 | 0.9955 | 0.1403 | 0.5629 |
DexMa-CsMa-Aam | 0.9799 | 0.9962 | 0.1612 | 0.5719 |
DexMa-CsMa-BisAam | 0.9843 | 0.9987 | 0.1822 | 0.5685 |
DexMa-GelMa | 0.9892 | 0.9904 | 0.1074 | 0.5270 |
DexMa-GelMa-Aam | 0.9792 | 0.9973 | 0.0934 | 0.5374 |
DexMa-GelMa-BisAam | 0.9765 | 0.9965 | 0.0962 | 0.5907 |
DexMa-XMa | 0.9803 | 0.9944 | 0.0962 | 0.5507 |
DexMa-XMa-Aam | 0.9789 | 0.9952 | 0.0943 | 0.5531 |
DexMa-XMa- BisAam | 0.9806 | 0.9986 | 0.1019 | 0.5496 |
Hydrogels | DexMa (%) | CsMa (%) | GelMa (%) | XMa (%) | Aam (%) | BisAam (%) |
---|---|---|---|---|---|---|
DexMa-CsMa | 50 | 50 | - | - | - | - |
DexMa-CsMa-Aam | 45 | 45 | - | - | 10 | |
DexMa-CsMa-BisAam | 45 | 45 | - | - | 8 | 2 |
DexMa-GelMa | 50 | - | 50 | - | - | - |
DexMa-GelMa-Aam | 45 | - | 45 | - | 10 | |
DexMa-GelMa-BisAam | 45 | - | 45 | - | 8 | 2 |
DexMa-XMa | 50 | - | - | 50 | - | - |
DexMa-XMa-Aam | 45 | - | - | 45 | 10 | |
DexMa-XMa-BisAam | 45 | - | - | 45 | 8 | 2 |
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Luca, A.; Nacu, I.; Tanasache, S.; Peptu, C.A.; Butnaru, M.; Verestiuc, L. New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy. Gels 2023, 9, 371. https://doi.org/10.3390/gels9050371
Luca A, Nacu I, Tanasache S, Peptu CA, Butnaru M, Verestiuc L. New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy. Gels. 2023; 9(5):371. https://doi.org/10.3390/gels9050371
Chicago/Turabian StyleLuca, Andreea, Isabella Nacu, Sabina Tanasache, Cătălina Anişoara Peptu, Maria Butnaru, and Liliana Verestiuc. 2023. "New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy" Gels 9, no. 5: 371. https://doi.org/10.3390/gels9050371