Agarase, Amylase and Xylanase from Halomonas meridiana: A Study on Optimization of Coproduction for Biomass Saccharification
Round 1
Reviewer 1 Report
Since the enzymes are coproduced in a mixture if only one of the enzymes is needed for a particular study, can this be accomplished? Please add a comment on this in conclusion or where needed.
Author Response
Please see the attachment.
Dear Sir/Madam,
The authors are very thankful for your review which helped us in improving our manuscript.
Author Response File: 
Author Response.docx
Reviewer 2 Report
Reviewer’s comments to the authors
Manuscript title: Agarase, Amylase and Xylanase: Pelagic to Production
Manuscript ID: fermentation-1872230
Recommendation: The authors strongly encouraged to clarify the novelty and importance of this work, I have doubts whether the implementation of the work has led to meaningful results, Therefore (Major revision is required).
The following are my comments and critique should be addressed before acceptance for publication:
Major comments:
# The authors needs to show the novelty of their work
# The accession numbers SUB11224620 and Halomonas meridiana VITSVRP14 its difficult to find in the GenBank database, the authors should explain ?
# Its highly recommended to check and reconstruct the phylogenetic tree ? after checking the accession numbers
# The title should be more informative
# As a non-native speaker, I found the manuscript easy to read and understand. However, there are some grammatical errors and in some instances, the phrasing need to improve.
# Lack of discussion part as a separate section, however, its merged with the results but its not clear and the authors should improve the discussion part
Minor comments:
# Affiliations should be corrected “numbers and address”. the authors not well followed the journal guidelines style
# Lines 14 and 15, the authors should clarify which enzymes value ? or you mean respectively ?
# At the end of the abstract, its prefer to show the importance of your work (flashing conclusions and recommendations)
# Why only these agarase, amylase, and xylanase enzymes were selected for the study ?
# Lines 30-32 and line 45 add more references (see similar situations).
# Microbial scientific names should be in italic
# Normalize the writing style “&” or “ and”; “co-production”or “coproduction” and other instances
# Define abbreviations upon first appearance in the text. Do it in the the whole article, in particular scientific names
# Dilution range ?
# Zobell marine agar and nutrient agar plates: write full chemical composition
# The authors should clarify the bacterial culture condition in details (lines 97-98)
# Line 102, temperature ?
# gram should be Gram (line 108)
# 16SrRNA should be 16S rRNA
# Lines 113-117, add reference(s).
# Lake of statistical data such as error bars or SD on the figure legends
# Improve table legends
# Add space between words and values/number, check your paper carefully
# The recent articles listed below can be reviewed and added to the References:
1. Hassan Mohamed, Abdallah Hassane, Omar Atta, Yuanda Song. Deep learning strategies for active secondary metabolites biosynthesis from fungi: Harnessing artificial manipulation and application. Biocatalysis and Agricultural Biotechnology 38, 102195 (2021). https://doi.org/10.1016/j.bcab.2021.102195
2. Zhang Z, Shah AM, Mohamed H, Zhang Y, Tsiklauri N, Song Y. Genomic Studies of White-Rot Fungus Cerrena unicolor SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass. J Fungi (Basel). 2021 Oct 5;7(10):835. doi: 10.3390/jof7100835. PMID: 34682256; PMCID: PMC8541250.
3. Hassan Mohamed, Aabid Manzoor Shah, Yuanda Song. Conversion of Agro-industrial Wastes into Value-Added Products. ACS Symposium Series; Chapter 10, 197-217. 2021. (Book Chapter). https://doi.org/10.1021/bk-2021-1392.ch010.
4. Zichen Zhang, Aabid Manzoor Shah, Hassan Mohamed, Yao Zhang, Beenish Sadaqat, Nino Tsiklauri, Tinatin Sadunishvili. Yuanda Song. Improved laccase production in Pleurotus djamor RP by atmospheric and room temperature plasma (ARTP) mutagenesis. Electronic Journal of Biotechnology. 58, 1-9. (2022). https://doi.org/10.1016/j.ejbt.2022.03.005
Author Response
Please see the attachment.
Dear Sir/Madam,
The authors are very thankful for your review which helped us in improving our manuscript.
Author Response File: Author Response.docx
