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Fermentation
Volume 9
Issue 8
10.3390/fermentation9080764
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Communication
Peer-Review Record

Biosynthesis of Glucaric Acid by Recombinant Strain of Escherichia coli Expressing Two Different Urinate Dehydrogenases

Fermentation 2023, 9(8), 764; https://doi.org/10.3390/fermentation9080764
by Xinchao Yang 1,†, Linlin Niu 2,†, Chunjiang Ye 1,*, Yuanxiu Wang 1, Yuehui Liu 1, Fang Wang 1 and Naxin Sun 1
Reviewer 1:
Pradeep Kumar Panda
Fermentation 2023, 9(8), 764; https://doi.org/10.3390/fermentation9080764
Submission received: 27 June 2023 / Revised: 24 July 2023 / Accepted: 7 August 2023 / Published: 17 August 2023
(This article belongs to the Special Issue Optimization and Analysis of Fermentation Process for Microbial Products)

Round 1

Reviewer 1 Report

Line 21, please make a space between the number and oC. Hours should be written as h. Check these errors throughout the manuscript.

Line 29, citation 16?

Highlight the novelty in the introduction section.

In each citation, authors wrote author et al. Please remove all places.

One abbreviation is defined many times please remove it. for ex: urinate dehydrogenase (Udh) this one used in line 83 and line 85 and other places also.

Write ml as m and μl as μL.

Line 248, figure is what? what is x and y-axis. Write in English.

The discussion section is too short. It is better to merge in the result section.

Each discussion needs to be improv critically. I don’t find any informative discussion.

 

English needs to be revised.

English is very difficult to understand/incomprehensible

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

However, my main concerns are

In Fig 2.2 A&B, and Fig 3.3 are poorly labeled, making it difficult to know what is being presented.

Figure 3.6. gluconic acid can be detected in the fermentation fluid of recombinant strains y and x axis present in the different language in manuscript. Authors need to represent the results in text.

Figure 3.6. Footnotes are not given.

Section 4 Discussion change in to conclusion.

Authors are not following journal guidelines.

The results and discussion need to be presented in a clear manner, presenting a summary or results and their relation to the published literature. Please make tighter connections to the results obtained and the discussion.

The results explanation is very limited please clarify it in detail. Discussion only repeats the results, without any interpretation.

Discussion is missing, explain in more details.

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

The authors improved the manuscript in a satisfactory level. 

Typos errors need to check.

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