Next Article in Journal
Chloroplast Genome Profiling and Phylogenetic Insights of the “Qixiadaxiangshui” Pear (Pyrus bretschneideri Rehd.1)
Next Article in Special Issue
The Production of Useful Phenol Compounds with Antioxidant Potential in Gametophytes and Sporophytes from In Vitro Cultures in Four Ornamental Ferns Species
Previous Article in Journal
OMC-YOLO: A Lightweight Grading Detection Method for Oyster Mushrooms
Previous Article in Special Issue
Effect of Foliar Selenate Supplementation on Biochemical Characteristics of Purslane Weed (Portulaca oleracea L.)
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability

by
Oana-Elena Pascariu
1,*,
Luís Guimarães Dias
2 and
Florentina Israel-Roming
1
1
Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Mărăști Blvd, District 1, 011464 Bucharest, Romania
2
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, 5300-253 Bragança, Portugal
*
Author to whom correspondence should be addressed.
Horticulturae 2024, 10(7), 743; https://doi.org/10.3390/horticulturae10070743
Submission received: 7 June 2024 / Revised: 8 July 2024 / Accepted: 12 July 2024 / Published: 15 July 2024
(This article belongs to the Special Issue Bioactive Compounds in Horticultural Plants—2nd Edition)

Abstract

Phenolic compounds from elderberries (Sambucus nigra L.) have attracted attention due to their potential health benefits. This paper examines different extraction methods used to obtain phenolic compounds from these fruits and the stability of the extracts. Several extraction techniques (extraction with continuous agitation, ultrasound-assisted extraction, microwave-assisted extraction, maceration, and enzyme-assisted extraction) were tested and compared to evaluate the yield and the quality of the extracts. The stability of the extracts with various storage parameters (time and temperature) and processing conditions (concentration and lyophilization) was also investigated. The results showed that ultrasound-assisted extraction (UAE) provided the highest yield of total phenolic compounds (74.89 mg GAE/g), of which 71.23% were represented by total anthocyanins and 62.50% by monomeric anthocyanins, with total flavonoids of 8.11–9.41 mg RUE/g. The analysis of individual phenolic compounds reconfirms the efficiency of UAE, obtaining 0.42–0.09 mg/g gallic acid, 0.59–0.01 mg/g chlorogenic acid, 0.17–0.03 mg/g 4-coumaric acid, and 2.43–0.01 mg/g rutin. The optimization of the extraction conditions led to the conclusion that the best solvent is 45% ethanol (v/v), and the optimal parameters are 40 °C for 40 min. It was also found that the stability of the extracts can be high during long periods of time (even after 180 days). These findings contribute to the understanding of the optimization of extraction processes and storage conditions to obtain extracts rich in phenolic compounds from elderberries, with potential uses in pharmaceutical and food applications.
Keywords: Sambucus nigra; bioactive compounds; extraction; phenolic compounds Sambucus nigra; bioactive compounds; extraction; phenolic compounds
Graphical Abstract

Share and Cite

MDPI and ACS Style

Pascariu, O.-E.; Dias, L.G.; Israel-Roming, F. Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability. Horticulturae 2024, 10, 743. https://doi.org/10.3390/horticulturae10070743

AMA Style

Pascariu O-E, Dias LG, Israel-Roming F. Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability. Horticulturae. 2024; 10(7):743. https://doi.org/10.3390/horticulturae10070743

Chicago/Turabian Style

Pascariu, Oana-Elena, Luís Guimarães Dias, and Florentina Israel-Roming. 2024. "Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability" Horticulturae 10, no. 7: 743. https://doi.org/10.3390/horticulturae10070743

APA Style

Pascariu, O.-E., Dias, L. G., & Israel-Roming, F. (2024). Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability. Horticulturae, 10(7), 743. https://doi.org/10.3390/horticulturae10070743

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop