Aequorin-Based In Vivo Luminescence Imaging Detects Calcium Signalling in Response to Biotic and Abiotic Stresses in Tomato
Abstract
:1. Introduction
2. Materials and Methods
2.1. Vector Construction and Transformation in Tomato
2.2. Plant Material and Growth Conditions
2.3. RNA Extraction and qPCR Analysis
2.4. Aequorin Reconstitution and Luminescence Imaging
2.5. Statistical Analysis
3. Results
3.1. Characterization of Transgenic Tomato Expressing Apoaequorin
3.2. [Ca2+]i Increase in Tomato Roots Can Be Monitored under NaCl/elf18 Treatment
3.3. [Ca2+]i Increase in Tomato Roots and Leaves Can Be Monitored under H2O2 or Pep1 Treatment
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequence (5′→3′) |
---|---|
Apoaequorin | F: ATGACCAGCGAACAATACTC |
R: TTAGGGGACAGCTCCACCGT | |
SlActin | F: TCGTAAGGAGTGCCCTAATGCTGA |
R: CAATCGCCTCCAGCCTTGTTGTAA |
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Liu, L.; Li, X.; Teng, Y.; Liu, X. Aequorin-Based In Vivo Luminescence Imaging Detects Calcium Signalling in Response to Biotic and Abiotic Stresses in Tomato. Horticulturae 2024, 10, 857. https://doi.org/10.3390/horticulturae10080857
Liu L, Li X, Teng Y, Liu X. Aequorin-Based In Vivo Luminescence Imaging Detects Calcium Signalling in Response to Biotic and Abiotic Stresses in Tomato. Horticulturae. 2024; 10(8):857. https://doi.org/10.3390/horticulturae10080857
Chicago/Turabian StyleLiu, Lulu, Xiaofei Li, Yibo Teng, and Xunyan Liu. 2024. "Aequorin-Based In Vivo Luminescence Imaging Detects Calcium Signalling in Response to Biotic and Abiotic Stresses in Tomato" Horticulturae 10, no. 8: 857. https://doi.org/10.3390/horticulturae10080857