Functional Identification of the Isopentenyl Diphosphate Isomerase Gene from Fritillaria unibracteata
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.1.1. Strain and Plasmid
2.1.2. Reagents and Plant Materials
2.1.3. Experimental Apparatus
2.2. Experimental Methods
2.2.1. Construction of pTrc-FuIPI Expression Plasmid and Expression Host
2.2.2. Color Complementation Experiment
2.2.3. Determination of β-Carotene Content
2.2.4. Site Mutation at Glu190 Residue
2.2.5. Subcellular Localization of FuIPI Protein
2.2.6. Construction and Identification of FuIPI Transgenic A. thaliana
2.2.7. Phenotypic Observation of FuIPI Transgenic Plants under Stress
2.2.8. Determination of Abscisic Acid (ABA) Content
3. Result
3.1. Functional Identification of FuIPI Gene in E. coli
3.2. Subcellular Localization of FuIPI
3.3. Production of FuIPI Transgenic Plants
3.4. FuIPI Transgenic A. thaliana Strengthened Tolerance to Drought and Salinity Stress
3.5. Overexpression of the FuIPI Gene Enhanced the Accumulation of ABA Content
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Software | Prediction Result |
---|---|
iPSORT | mitochondrial targeting or chloroplast transport peptide |
Plant-mPLoc | chloroplast |
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Yu, X.; Chen, J.; Yan, H.; Huang, X.; Chen, J.; Ma, Z.; Zhou, J.; Liao, H. Functional Identification of the Isopentenyl Diphosphate Isomerase Gene from Fritillaria unibracteata. Horticulturae 2024, 10, 887. https://doi.org/10.3390/horticulturae10080887
Yu X, Chen J, Yan H, Huang X, Chen J, Ma Z, Zhou J, Liao H. Functional Identification of the Isopentenyl Diphosphate Isomerase Gene from Fritillaria unibracteata. Horticulturae. 2024; 10(8):887. https://doi.org/10.3390/horticulturae10080887
Chicago/Turabian StyleYu, Xinyi, Jiao Chen, Han Yan, Xue Huang, Jieru Chen, Zichun Ma, Jiayu Zhou, and Hai Liao. 2024. "Functional Identification of the Isopentenyl Diphosphate Isomerase Gene from Fritillaria unibracteata" Horticulturae 10, no. 8: 887. https://doi.org/10.3390/horticulturae10080887