Costimulatory Molecules CD80/86 Trigger Non-Specific Cytotoxic Cell of Nile tilapia (Oreochromis niloticus) to Kill CIK Cells
, Zhengsi Chen 1,2,3, Ruitao Xie 4,5, Kwaku Amoah 1,2,3, Bei Wang 1,2,3, Jia Cai 1,2,3, Yishan Lu 1,2,3 and Jichang Jian 1,2,3,*
Round 1
Reviewer 1 Report
This manuscript describes how CD80/86 triggers the tilapia NCC functions. The study is interesting and important to ascertain fish NCC functions.
The manuscript really needs to be improved in the writing and edition. A native speaker or revisor is encouraged.
Comments:
- In section 2.4. it is not explained why you have to stimulate with 5C6 before the addition of the rOn-CD80/86. No control or nonstimulated leucocytes are included as proper controls.
- Section 2.5. The NCC killing assay was performed with 1 NCC:1 CIK cells. This is very low and uncommon for fish NCCs. Additionally, the method used to determine the NCC killing activity is “rare” and uncited. Please, explain them.
- Line 196-198. “….Nile tilapia head kidney lymphocytes (HKLs) can be divided into four subgroups, including nonspecific NCC cells, T cells and macrophage/monocyte (Mo/Mø)”. You indicate 4 populations and then describe 3, and macrophage/monocyte are not lymphocytes. Please, revise this.
- The number of fish used is not indicated. Are NCCs from individual fish or pooled?
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Revision for an article titled: Costimulatory Molecules CD80/86 Trigger Nonspecific Cytotoxic Cell of Nile tilapia (Oreochromis niloticus) to Kill 3CIK Cells
This paper is interesting and addresses a new important topic, however, minor revision is required:
In the title: Please add a dash in-between non and specific (Non-specific) instead of Nonspecific
Please be consistent in using either Nile tilapia or Oreochromis niloticus, I observed that you used mostly Nile tilapia so please replace Oreochromis niloticus in lines 24, 29, 376 by Nile tilapia.
Line38 Please add a dash in-between non and specific (Non-specific) instead of Nonspecific
Line 67: Please change the words Streptococcus agalactiae and Aeromonas hydrophila to italic.
Line 73: Please change the word Escherichia coli to italic.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 3 Report
The manuscript with ID (fishes-2035098) by Huang and coauthors has evaluated the costimulatory Molecules CD80/86 that trigger the non-specific cytotoxic cell of Nile tilapia to kill CIK Cells. The manuscript is interesting; however, there are several major issues to be revised by the authors prior the manuscript considered for publication in Fishes.
Ø The manuscript in general needs to be sent to a native English Speaker. MDPI has an English Editing and proof-reading system. This is because there are several grammar mistakes throughout the whole manuscript.
Ø All my revisions have been included in the text of the PDF file with Track changes and highlights. Authors have to revise them very carefully and respond to all of them in a detailed response letter.
References
§ This section needs extensive revisions.
§ Several references missed the Journal names.
§ Most of journal names written incorrect.
§ All Latin names must be written italic
§ Authors have to revise the PDF file very carefully.
Supplementary files:
§ Table S1. The primer sequences that used in this research.
§ Table S2. NCBI GenBank Accession Numbers of the target mRNA relative genes of Nile tilapia that used in this study.
§ These tables should be presented inside the text.
§ Primer efficiency should be also included in Table S2.
Comments for author File: Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Authors have improved the manuscript though English could be much improved.
Reviewer 3 Report
Authors have properly addressed my comments in the modified manuscript
