Next Article in Journal
Development of an ICP-MS/MS-Based Methodology for the Analysis of (Ultra) Trace Elements in Follicular Fluid Samples of Patients Undergoing IVF Treatment
Previous Article in Journal
Effects of Non-Lethal Arsenic Contamination on Manifesting Fin Strokes in Zebrafish (Danio rerio)
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Proceedings
Volume 102
Issue 1
10.3390/proceedings2024102035
proceedings-logo
Submit to this Journal
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Abstract

Thiram Effects on HeLa TI Cells †

by
Varvara Maksimova
1,*,
Anzhelika Bukina
1,2,
Guzel Khayrieva
3,
Valeriia Popova
1,
Marianna Yakubovskaya
1 and
Kirill Kirsanov
1
1
Department of Chemical Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia
2
Department of Biotechnology and Industrial Pharmacy, Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 86 Vernadsky Ave., 119571 Moscow, Russia
3
Institute of Clinical Medicine, I.M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya Str., 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
†
Presented at the 1st International Electronic Conference on Toxics, 20–22 March 2024; Available online: https://sciforum.net/event/IECTO2024.
Proceedings 2024, 102(1), 35; https://doi.org/10.3390/proceedings2024102035
Published: 3 April 2024
Versions Notes

1. Introduction

Dithiocarbamate pesticides possess a diverse array of molecular mechanisms, making them multifunctional substances. Among the commonly used dithiocarbamates, there is the fungicide thiram, employed for safeguarding plants and seeds against fungal diseases. The limited solubility of thiram in water facilitates its accumulation in the soil, thereby raising concerns about its potential impact on human health. Despite the widespread use of thiram, there is still a paucity of knowledge regarding its toxic effects on humans. This study aimed to assess the genotoxic effects of thiram, its influence on cell colony formation, and its effect on the expression profile of genes associated with proliferation and repair. Additionally, we conducted an analysis of thiram’s integral epigenetic activity under different time exposures.

2. Materials and Methods

HeLa TI cells were used in this study. DNA damage was assessed using the DNA comet assay, while the clonogenic assay was utilized to evaluate the colony-forming ability of cells after thiram treatment. Gene expression levels were measured using real-time PCR. The epigenetic activity of thiram was assessed by quantifying the reactivation level of the epigenetically silenced GFP gene utilizing flow cytometry.

3. Results

Using HeLa TI cells, we observed that thiram significantly increased tail moment in the DNA comet assay and caused a significant decrease in cell survival as determined by the clonogenic assay. Additionally, thiram downregulated the gene expression levels of DDB2, ERCC5, LIG4, and WEE1, while upregulating the expression levels of MCM2, DDIT3, ERCC3, JUN1, and ESR1. The analysis of epigenetic activity revealed that thiram induced a statistically significant increase in the GFP+ cells amount after exposures of 24, 72, and 96 h.

4. Conclusions

Thus, thiram exhibits genotoxic and cytotoxic effects, influences the expression of proliferation and repair genes, and induces dose- and time-dependent epigenetic modifications in HeLa TI cells.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/proceedings2024102035/s1.

Author Contributions

Conceptualization, V.M., M.Y. and K.K.; methodology, V.M.; formal analysis, V.M., A.B., V.P. and G.K.; investigation, V.M., A.B., V.P. and G.K.; resources, K.K.; data curation, V.M.; writing—original draft preparation, V.M.; writing—review and editing, M.Y. and K.K.; visualization, V.M.; supervision, K.K.; project administration, V.M.; funding acquisition, V.M. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Russian Scientific Foundation, grant number 23-25-00541.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data available on request.

Conflicts of Interest

The authors declare no conflict of interest.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Maksimova, V.; Bukina, A.; Khayrieva, G.; Popova, V.; Yakubovskaya, M.; Kirsanov, K. Thiram Effects on HeLa TI Cells. Proceedings 2024, 102, 35. https://doi.org/10.3390/proceedings2024102035

AMA Style

Maksimova V, Bukina A, Khayrieva G, Popova V, Yakubovskaya M, Kirsanov K. Thiram Effects on HeLa TI Cells. Proceedings. 2024; 102(1):35. https://doi.org/10.3390/proceedings2024102035

Chicago/Turabian Style

Maksimova, Varvara, Anzhelika Bukina, Guzel Khayrieva, Valeriia Popova, Marianna Yakubovskaya, and Kirill Kirsanov. 2024. "Thiram Effects on HeLa TI Cells" Proceedings 102, no. 1: 35. https://doi.org/10.3390/proceedings2024102035

APA Style

Maksimova, V., Bukina, A., Khayrieva, G., Popova, V., Yakubovskaya, M., & Kirsanov, K. (2024). Thiram Effects on HeLa TI Cells. Proceedings, 102(1), 35. https://doi.org/10.3390/proceedings2024102035

Article Metrics

Back to TopTop