Chronic prenatal hypoxia (CPH) is one of the most common causes of neonatal mortality and postnatal disorders of the nervous system and the mental development of infants. The search for new methods to treat the effects of CPH is a high-priority problem of modern pharmacology. HIF-1α in hypoxic conditions activates mechanisms of endogenous neuroprotection and can be used as a target for pharmacological correction.
Aim of the work: To study the possibilities of the HIF-1α-modulating effects of different pharmacological agents in the model of CPH.
CPH was modeled by administering sodium nitrite (50 mg/kg) to pregnant female white rats from day 16 of gestation. The offspring were treated with the following drugs: piracetam, thiotriazoline, nikomex, tamoxifen, cerebrocurin, angiolin, glutoredoxin, l-arginine, and HSF-1 during the first 30 days of life. The concentration of HIF-1α in the plasma and brain homogenate of rats at 30 and 60 days of life was determined using the enzyme-linked immunosorbent assay.
It was found that CPH leads to a decrease in the concentration of HIF-1α in the nervous tissue of the brain and blood plasma. Drug administration results in a significant increase in the content of HIF-1α in the studied objects immediately at the end of treatment with continued positive effects on the 60th day of life. The maximum effect was demonstrated on day 30 by cerebrocurin, angiolin, and HSF1, and on day 60 by cerebrocurin, angiolin, and thiatriazolin.
Our research provides evidence that the medications being tested are an effective way to modulate HIF-1α and may be a new alternative for the treatment and prevention of nervous system disorders in children caused by CPH.
Author Contributions
Conceptualization, methodology, I.F.B. and O.G.A.; conducting an experiment, O.G.A. and O.O.P.; data curation, O.O.P.; writing—original draft preparation, O.G.A.; software, O.G.A.; supervision, project administration, I.F.B. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review Board Statement
The experimental studies were carried out in accordance with the “Regulations on the Use of Animals in Biomedical Research” and with the European Convention on the Protection of Animals Used for Scientific and Other Purposes. The experiment was approved by the Bioethics Committee of Zaporizhzhia State Medical and Pharmaceutical University.
Informed Consent Statement
Not applicable.
Data Availability Statement
The data are available from the corresponding author upon reasonable request.
Conflicts of Interest
The authors declare no conflict of interest.
| Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
