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Abstract

Investigation of the Effects of Momordica charantia Extract on Cell Survival and Migration in U87G Glioblastoma Cell Line †

1
Department of Biotechnology, Institute of Science, Bilecik Seyh Edebali University, 11030 Bilecik, Turkey
2
Department of Molecular Biology and Genetics, Faculty of Art and Science, Bilecik Seyh Edebali University, 11030 Bilecik, Turkey
3
Biotechnology Research and Application Center, Bilecik Seyh Edebali University, 11030 Bilecik, Turkey
*
Author to whom correspondence should be addressed.
Presented at the 3rd International conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 18–20 December 2019.
Proceedings 2019, 40(1), 18; https://doi.org/10.3390/proceedings2019040018
Published: 26 December 2019

Abstract

:
Glioblastoma multiforme (GBM) is a type of cancer which has the highest mortality rate among brain cancers (1–2). Momordica charantia, known as bitter melon, is a plant its pharmacological activities and nutritional properties. Due to contains bioactive compounds, M. charantia is used for cancer treatments, inflammation-related diseases and diabetes (3–4). In this study, it was aimed to investigate the effects of M. charantia extract on cell viability, cytotoxicity and migration capacity in U87G cell line. U87G was cultured in DMEM-high glucose containing FBS 10% (v/v) and penisillin-streptomicin 1% (v/v). Cells were incubated at 37 °C in a humidified 5% CO2 incubator. The cytotoxic effect of M. charantia extract was determined by MTT analysis, cell viability by survival analysis and migration by wound-healing analysis. The results were evaluated by using ANOVA and GraphPad Prism7.0 program (GraphPad Software, La Jolla, CA, USA) in three replicates. IC50 value of M. charantia extract was found 750 μg/mL which is statistically significant (* p < 0.05). The extract had an increasing lethal effect at the 16.6% (24 h), 42.6% (48 h), 79.3% (72 h) and 91.6% (96 h). According to the wound-healing analysis, the wound closed at 24 h in the control group and the wound gradually increased depending on time in the extract treated group. According to the results, M. charantia extract has a cytotoxic and a significant anti-proliferative effect on U87G. It might be used as therapeutic agent against to GBM. However, in order to understand the effect of M. charantia in living organisms, in vivo experiments must be determined.

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MDPI and ACS Style

Erdogan, K.; Eroglu, O. Investigation of the Effects of Momordica charantia Extract on Cell Survival and Migration in U87G Glioblastoma Cell Line. Proceedings 2019, 40, 18. https://doi.org/10.3390/proceedings2019040018

AMA Style

Erdogan K, Eroglu O. Investigation of the Effects of Momordica charantia Extract on Cell Survival and Migration in U87G Glioblastoma Cell Line. Proceedings. 2019; 40(1):18. https://doi.org/10.3390/proceedings2019040018

Chicago/Turabian Style

Erdogan, Kubra, and Onur Eroglu. 2019. "Investigation of the Effects of Momordica charantia Extract on Cell Survival and Migration in U87G Glioblastoma Cell Line" Proceedings 40, no. 1: 18. https://doi.org/10.3390/proceedings2019040018

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