Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure †
by
, ,
Stefania Noerman
1,*
,
Marina Armeni
1,2,
Giuseppe Di Pede
3,
Raul Gonzalez-Domínguez
4,
Otto Savolainen
2,5 and
Rikard Landberg
1 1
Food and Nutrition Science Division, Department of Life Sciences, Chalmers University of Technology, 41258 Gothenburg, Sweden
2
Chalmers Mass Spectrometry Infrastructure, Department of Life Sciences, Chalmers University of Technology, 41258 Gothenburg, Sweden
3
Department of Food and Drugs, University of Parma, 43121 Parma, Italy
4
Instituto de Investigación e Innovación Biomédica de Cádiz (INiBICA), Hospital Universitario Puerta del Mar, Universidad de Cádiz, 11009 Cádiz, Spain
5
Institute of Public Health and Clinical Nutrition, University of Eastern Finland, 70210 Kuopio, Finland
*
Author to whom correspondence should be addressed.
†
Presented at the 14th European Nutrition Conference FENS 2023, Belgrade, Serbia, 14–17 November 2023.
Proceedings 2023, 91(1), 421; https://doi.org/10.3390/proceedings2023091421
Published: 8 April 2024
(This article belongs to the Proceedings of The 14th European Nutrition Conference FENS 2023)
Abstract
:Assessment of dietary intake remains a large challenge in nutrition studies. The application of food intake biomarkers is a promising approach to complement widely used self-reported intake assessments and to improve accuracy. The development of metabolomics has enabled the discovery of many potential food intake biomarkers, but their applications are still limited. We aim to develop a semi-quantitative LC-MS/MS procedure to analyze a panel of plasma metabolites reflecting dietary exposure in a wide context. Our approach relies on a multi-analyte targeted LC-MS/MS method using a LC-QTRAP and commercially available reference compounds. A panel of 347 metabolites was selected, representing dietary intake (fruits and vegetables, coffee, tea, heat-treated food, wholegrain cereals, berries, cruciferous vegetables, and seafood) and key metabolites in the endogenous metabolism (fatty acids, amino acids, cholesterol metabolism, Krebs cycle, bile acids, and microbial metabolism) which are affected by specific diets, as well as lifestyle exposures, such as smoking and alcohol consumption. The application of this panel will help in assessing dietary exposures and their relationships to health outcomes. We will present the status of the work.
Author Contributions
Conceptualization, R.L. and O.S.; methodology, M.A. and R.G.-D.; software, S.N.; validation, M.A.; formal analysis, G.D.P. and M.A.; data curation, S.N.; writing—original draft preparation, S.N.; writing—review and editing, M.A., O.S. and R.L.; funding acquisition, R.G.-D. and R.L. All authors have read and agreed to the published version of the manuscript.
Funding
S.N. received funding from Formas (Dnr: 2019-02201) under the umbrella of the European Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL) and of the ERA-NET Cofund HDHL INTIMIC (GA N° 727565 of the EU Horizon 2020 Research and Innovation Programme). R.G.-D. is recipient of a “Miguel Servet” fellowship (CP21/00120) funded by “Instituto de Salud Carlos III”. R.L. is funded by the Swedish Research Council (no 2019-12064).
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Data sharing is not applicable to this abstract.
Conflicts of Interest
The authors declare no conflict of interest.
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Share and Cite
MDPI and ACS Style
Noerman, S.; Armeni, M.; Di Pede, G.; Gonzalez-Domínguez, R.; Savolainen, O.; Landberg, R. Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure. Proceedings 2023, 91, 421. https://doi.org/10.3390/proceedings2023091421
AMA Style
Noerman S, Armeni M, Di Pede G, Gonzalez-Domínguez R, Savolainen O, Landberg R. Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure. Proceedings. 2023; 91(1):421. https://doi.org/10.3390/proceedings2023091421
Chicago/Turabian StyleNoerman, Stefania, Marina Armeni, Giuseppe Di Pede, Raul Gonzalez-Domínguez, Otto Savolainen, and Rikard Landberg. 2023. "Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure" Proceedings 91, no. 1: 421. https://doi.org/10.3390/proceedings2023091421
