Influence of Temperature and LED Light Spectra on Flavonoid Contents in Poa pratensis

Light and temperature are the driving forces in plant development and growth. Specific photoreceptors provide the ability to sense and interpret light and temperature to regulate growth. Under the limited light conditions in most sports stadiums, natural grasses suffer from light deficiency. Artificial light provided by light-emitting diodes (LEDs) is used to increase their growth and adjust their development. Flavonoids like flavonols and anthocyanins are influenced by light conditions and temperature. Increased blue light can elevate the content of these secondary metabolites. Remote measurements of internal parameters using non-destructive methods provided information on their content under different temperature conditions for quality monitoring. This experiment tested flavonoid contents in Kentucky bluegrass (Poa pratensis) for different blue-to-red light ratios (0.6 and 0.4) and three temperature courses (constant temperature of 4 °C, constant temperature of 12 °C, and temperature switching among 12–8–4–8–12 °C). The results show elevated levels of flavonoids under blue-dominant artificial light as well as increased content under low-temperature (4 °C) conditions. The lack of flavonoids at elevated temperatures (12 °C), especially under red-dominant light, suggests an increased requirement for artificial blue light at increased temperatures. Non-destructive flavonoid determination was suitable for this experiment and can therefore be used for practical sports turf quality monitoring.