Round 1

Reviewer 1 Report (Previous Reviewer 3)

The author presented the paper "Bio-Medical Aspects of Nanoparticles". The paper was improved. However, I have some minor comments.

1) Reference list should be improved. I see that some new 2022-2023 review papers are greatly suitable for your work. I see that many works may be changed for a fresh one for the Introductions section. For example, see the search link in the MDPI below.

various nanocomposites with nucleic acid applications for lines 109-125

https://www.mdpi.com/search?sort=pubdate&page_count=50&year_from=2021&year_to=2023&article_types=review-article%2Cresearch-article&q=nanocomposite+nucleic+acid&view=default

albumin coated nanoparticles for lines 335-343

https://www.mdpi.com/search?sort=relevance&page_count=50&year_from=2022&year_to=2023&article_types=review-article&q=nanoparticle+albumin&view=default

Please also mention smth. about calcium carbonate nanoparticles for biomedical applications

https://www.mdpi.com/2306-5354/9/11/691

https://www.mdpi.com/1999-4923/15/3/771

Please, present more references for the sections 6 and 7.

2) Fig. 4. Please insert all mentioned in the text types of nanoparticles as magnetic, silica, biopolymer coated. Why you have selected carbon nanoparticles but not selected other important types, which you have mentioned as inorganic?

3) It will be good to present somewhere a Figure with summarized biomedical applications.

4) You have presented various types of nanoparticles. However, in the Conclusion section I see only carbon and gold. Please, present the material for all types and their various data and applications.

Minor editing of English language required

Author Response

Ref: electrochem- 2473945

 

Title: Bio-Medical Aspects of Nanoparticles

 

1. Reply to the Reviewer #1’s comments:

 

The author presented the paper "Bio-Medical Aspects of Nanoparticles". The paper was improved. However, I have some minor comments.

Reply: Thank you for the positive feedback.

1. The reference list should be improved. I see that some new 2022-2023 review papers are greatly suitable for your work. I see that many works may be changed for a fresh one for the Introductions section. For example, see the search link in the MDPI below.

various nanocomposites with nucleic acid applications for lines 109-125

https://www.mdpi.com/search?sort=pubdate&page_count=50&year_from=2021&year_to=2023&article_types=review-article%2Cresearch-article&q=nanocomposite+nucleic+acid&view=default

albumin coated nanoparticles for lines 335-343

https://www.mdpi.com/search?sort=relevance&page_count=50&year_from=2022&year_to=2023&article_types=review-article&q=nanoparticle+albumin&view=default

Please also mention smth. about calcium carbonate nanoparticles for biomedical applications

https://www.mdpi.com/2306-5354/9/11/691

https://www.mdpi.com/1999-4923/15/3/771

Please, present more references for sections 6 and 7.

 

Reply: Thanks for your critical comment. We agree with your point. The introduction line has been changed accordingly and new references 34, 35, 36, 76, 77, 83 and 84 etc have been suitable incorporated as suggested in the revised version of the manuscript.

 

2. 4. Please insert all mentioned in the text types of nanoparticles as magnetic, silica, biopolymer coated. Why you have selected carbon nanoparticles but not selected other important types, which you have mentioned as inorganic?

 

 

Reply: Thanks for the critical comment. We agree with your point. I have added suitably to various parts of the manuscript (writing content).

3. It will be good to present somewhere a Figure with summarized biomedical applications.

Reply: Thanks for your comment. Figure 2 accounts for broad applications for nanomaterials.  

4. You have presented various types of nanoparticles. However, in the Conclusion section, I see only carbon and gold. Please, present the material for all types and their various data and applications.

 

Reply: Thanks for your comment. A few sentences are incorporated to enhance the continuity of the article in the revised version of the manuscript.

 

Reviewer 2 Report (Previous Reviewer 2)

electrochem-2355971

Review of the review article titled: 

«Bio-Medical Aspects of Nanoparticles»

 

by MANSI GANDHI, Khairunnisa Amreen

 

In Electrochem (ISSN 2673-3293).

 

Round after resubmission

 

The abstract perfectly underlines the relevance of the presented studies.

 

The article was presented in a well-structured manner, with a good level of organization. Unfortunately, several statements within have weak evidence. Therefore, the referee suggested improving the manuscript with a major revision. The following is a list of specific concerns.

 

1.             Introduction. In the introduction section, it is necessary to clearly define the range of issues considered in the review and the period. 

2.             The review does not include recent achievements and advances in this area. Only five works have been cited here since 2019 (including) 100 in the review reference list. 2023 — 0; 2022 — 5.

3.             The titles of all paragraphs should be reconsidered. Also, it should be enlarged and give much more precise information.

4.             There are no significant changes to the manuscript.

5.             The impact of labile metal nanoparticles on cellular homeostasis should be considered. 

https://www.sciencedirect.com/science/article/abs/pii/S0304416516305062

Author Response

Ref: electrochem-2473945

 

Title: Bio-Medical Aspects of Nanoparticles

 

1. Reply to the Reviewer #2’s comments:

 

The abstract perfectly underlines the relevance of the presented studies. The article was presented in a well-structured manner, with a good level of organization. Unfortunately, several statements within have weak evidence. Therefore, the referee suggested improving the manuscript with a major revision. The following is a list of specific concerns.

Reply: Thanks for your positive feedback. We agree with your points. Changes have been made in the revised manuscript with additional evidences as suggested.

1. Introduction. In the introduction section, it is necessary to clearly define the range of issues considered in the review and the time period.

Reply: Thanks for your critical comment. The range of issues/topics is defined in the last few lines of the introduction (Lines 90 to 101).

2. Here it is also required to note whether there are reviews on similar topics in world literature of the last ten years. List them and indicate the principal differences between this review and other available ones. -The purpose of the Introduction is to attract the reader of Electrochem (MDPI) by what he can find here in contrast to similar publications.

There are a number of articles published on ‘nanomaterials’, ‘biomedical systems’, ‘metal based biomaterials in biomedical systems’, ‘controllable synthesis and biomedical applications of silver nanomaterials’, ‘advanced engineered nanomaterials for point-of-care immunosensing’ and so on and so forth. But a holistic research review from the start of nanomaterials, their characterization techniques, characteristic properties along with their applications in the domain of biomedical respect to its characterizations and properties, followed by their limitations and advantages, and finally their real-time applications used in industry products have not been published yet. This holistic approach makes this review article special and stand out compared to other articles. A small table comparing 20 articles published and the principal difference is as follows.

S. No.	Article Title	Article Emphasis	DOI
1.	Self-Assembled Fluorescent Organic Nanomaterials for Biomedical Imaging	Only relevant to self-assembled fluorescent contrast agents for biomedical imaging.	10.1002/adhm.201800344
2.	Conducting Polymer Nanomaterials for Biomedical Applications: Cellular Interfacing and Biosensing	Only regarding conducting polymers and their biomedical applications	10.1080/15583724.2013.805771
3.	Recent advances in biomedical applications of 2D nanomaterials with peroxidase-like properties	Classification of different types of 2D POD-like nanomaterials, and their properties for biomedical applications	10.1016/j.addr.2022.114269
4.	The Advances and Biomedical Applications of Imageable Nanomaterials	Emphasised on the characteristics of imageable nanomaterials, highlighted the advantages and biomedical applications of imageable nanomaterials in the diagnosis and treatment of diseases	10.3389/fbioe.2022.914105
5.	Role of Nanomaterials in the Fabrication of bioNEMS/MEMS for Biomedical Applications and towards Pioneering Food Waste Utilisation	his review aspires to communicate recent progress in organic and inorganic nanomaterials based bioNEMS/MEMS for biomedical applications, comprehensively discussing nanomaterials criteria and their prospects as ideal tools for biomedical devices.	10.3390/nano12224025
6.	Controllable Synthesis and Biomedical Applications of Silver Nanomaterials	controllable synthesis of silver nanomaterials including nanorods, nanowires, nanotubes, nanoprisms, nanoplates, nanodisks, nanospheres, and nanopolyhedrons, etc. are reviewed. Their shape specific properties like antibacterial and antiviral applications, antitumor applications, biosensors and biological labels, optical imaging and imaging intensifier, are discussed.	10.1166/jnn.2011.5317
7.	Graphene and Graphene-Based Materials in Biomedical Applications	strategies to synthesize graphene and its derivatives are mentioned. Biocompatibility profile of synthesised graphene nanomaterials as a precursor to their biomedical application are discussed.	10.2174/0929867326666190705155854
8.	Peptide-Engineered Fluorescent Nanomaterials: Structure Design, Function Tailoring, and Biomedical Applications	Describes potential strategies for the preparation of peptide-engineered fluorescent nanomaterials functionalization and biomodification strategies for biomedical applications.	10.1002/smll.202005578
9.	Nanomaterials based on thermosensitive polymer in biomedical field	research progress of thermosensitive nanomaterials as "intelligent " systems for biomedical applications.	10.3389/fchem.2022.946183
10.	Research trends in biomedical applications of two-dimensional nanomaterials over the last decade-A bibliometric analysis	the contributions of 2D nanomaterials to the various biomedical subfields are highlighted	10.1016/j.addr.2022.114420
11.	Recent Advances of Cell Membrane-Coated Nanomaterials for Biomedical Applications	cell membrane-coated nanomaterials are reviewed based on the main biological functions of the cell membrane in living cells and their perspectives of biomedical applications are summarised.	10.1002/adfm.202003559
12.	Multifaceted Biomedical Applications of Functional Graphene Nanomaterials to Coated Substrates, Patterned Arrays and Hybrid Scaffolds	biological potentials of functional graphene nanomaterials and summarize some of major literature concerning the multifaceted biomedical applications	10.3390/nano7110369
13.	Biomedical Effects and Nanosafety of Engineered Nanomaterials: Recent Progress	recent advance in the research on the biological effects of engineered nanomaterials and nanosafety issue, by focusing on the studies about representative nanomaterials	10.1002/cjoc.201200662
14.	Preparation, applications, and challenges of functional DNA nanomaterials	reparation methods of DNA nanomaterials with functions such as molecular recognition, nanozyme catalysis, drug delivery, and biomedical material templates	10.1007/s12274-022-4793-5
15.	Aptamer functionalized nanomaterials for biomedical applications: Recent advances and new horizons	versatile roles of nanomaterials in advanced aptamer functionalized nanomaterials are discussed, including optical indicators, conducting transducer, carriers, catalysts, and other functions.	10.1016/j.nantod.2021.101177
16.	Current Use of Carbon-Based Materials for Biomedical Applications-A Prospective and Review	recent studies in developing carbon-based nanomaterials and its applications in targeting drug delivery, cancer therapy, and biosensors are discussed.	10.3390/pr8030355
17.	Layered double hydroxide-based nanomaterials for biomedical applications	Layered double hydroxides based nanomaterials with versatile physicochemical advantages for biomedical applications are summarised	10.1039/d2cs00236a
18.	Microfluidic Synthesis of Nanomaterials for Biomedical Applications	design principles of microfluidic systems for nanomaterials synthesis is summarized. Following, microfluidic platforms are extended as new avenues for custom-made complex nanomaterials for biomedical applications	10.1002/smtd.201700140
19.	Nanomaterial integrated 3D printing for biomedical applications	nanomaterials have been incorporated into 3D printing polymers to fabricate innovative, versatile, multifunctional hybrid materials. The review focuses on recent advances in novel hybrid biomaterials composed of nanomaterials and 3D printing technologies for biomedical applications	10.1039/d2tb00931e
20.	The usage of composite nanomaterials in biomedical engineering applications	In this review, overview of the development of biosensors for the diagnosis of diseases, drug targeting and controlled release applications, medical implants and imaging techniques are the research topics of nanobiotechnology.	10.1002/bit.27843

2. The review does not include recent achievements and advances in this area. Only five works have been cited here since 2019 (including) 100 in the review reference list. 2023 — 0; 2022 — 5.

Reply: Thanks for your critical comment. We agree added new references 2023- 6; 2022-8; 2021- 5 in the revised version of manuscript.

3. The titles of all paragraphs should be reconsidered. Also, it should be enlarged and give much more precise information.

Reply: Thanks for your critical feedback. We have incorporated the suggestions in the revised version of manuscript.

4. There are no significant changes to the manuscript.

Reply: Thanks for your comment. All the suggestions are incorporated in the revised version of manuscript.

5. The impact of labile metal nanoparticles on cellular homeostasis should be considered. 

https://www.sciencedirect.com/science/article/abs/pii/S0304416516305062

Reply: Thanks for your comment. We have incorporated the same in the revised version of manuscript.

 

 

 

Author Response File: Author Response.docx

Round 2

Reviewer 2 Report (Previous Reviewer 2)

electrochem-2473945

Review of the review article titled: 

«Bio-Medical Aspects of Nanoparticles»

 

by MANSI GANDHI, Khairunnisa Amreen

 

In Electrochem (ISSN 2673-3293).

 

Round 2 after resubmission

 

 

The paper has been improved, enlarged, and clarified. Still, I have some minor comments presented below. This review should be reevaluated after minor revision.

 

1.     The title of the article still has uncleared. I would be recommended to improve and re-title this article.

 

2.     The novelty statement of this review is unclear. Please provide a new paragraph in the introduction section. 

 

3.     The authors should be double-checked English grammar and spelling.

Author Response

Ref: electrochem- 2473945

 

Title: Bio-Medical Aspects of Nanoparticles

 

1. Reply to the Reviewer #2’s comments:

The paper has been improved, enlarged, and clarified. Still, I have some minor comments presented below. This review should be reevaluated after minor revision.

Reply: Thank you for the positive feedback.

1. The title of the article still has uncleared. I would be recommended to improve and re-title this article.

Reply: Thanks for your critical comment. We have updated as suggested by you.

2. The novelty statement of this review is unclear. Please provide a new paragraph in the introduction section. 

 

Reply: Thanks for the critical comment. We agree with your point. We have suitably incorporated in the revised version.

3. The authors should be double-checked English grammar and spelling.

Reply: Thanks for your comment. We have made the necessary changes in the revised version of manuscript.

Author Response File: Author Response.docx

This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.

Round 1

Reviewer 1 Report

I have many issues with this manuscript and do not recommend its publication.

 

1)    The stated goal (l. 87-90 p.3) is way too ambitious and impossible to achieve in a single review. Also, that stated goal does not fit the article title.

 

2)     In my view, the authors keep dispensing “information” in every direction without any transition. It makes the article difficult to read. It also makes it difficult to learn something from reading it.

 

Here is an example (l. 617-621 p. 17):

AuNP’s have same material to that of old jewelry, but the various shapes of AuNP allows various colors compared to yellow for bulk gold, due to interaction of light and further reflectance.

Here the authors seem to discuss the surface plasmon resonance properties of gold nanoparticles, which depend on nanoparticle size, shape, and surface adsorbents. I don’t see how the reader can understand what they are saying without already having the knowledge.

These different colors can be employed as biomarkers, fluorescent inducers for diagnosis of heath related conditions.

Surface functionalization can be used to detect analytes. The latter involves either nanoparticle growth or aggregation, which affects the nanoparticle optical properties. Again, I don’t see how the reader can understand it from this writing.

Many metal based systems such as copper which is extremely rigid at nano level can be a ductile wire in bulk metal.

What does this mean and how does it relate to the previous sentence?

 

3)    The cartoonish figures are of poor quality.

 

4)    Not all of the “information” dispensed in the article is properly referred to.

 

Here is an example (l. 167-179 p.5):

Precisely choosing of nanomaterials as a promising drug delivery platform that can help in altering the hydrophobicity, thereby enhancing the water-solubility and upgrading their bio-availability characteristics. It is acquired under the passive or active targeting of drugs based tailored systems that are quite responsive for stimuli such as optical, thermal and hydrogen ion concentrations changes. Owing to minute size-exclusion, they infiltrate through the capillaries to the targeted tumor sites where they accumulate and helps in decreasing/reduction in the after-effects i.e. mainly side effect and toxicity issues along with accelerating their efficacy. Representing features of controlled release, prolonged action of work, balance in drug levels and balancing the therapeutic window, thereby avoiding hazardous drug concentration in the organs. The choice of co-delivery of more than one for simultaneous therapy and diagnosis,[40,41] cumulatively incorporating traits of synergistic effects, tuning relative therapeutic regime, drug resistance, etc. is possible using nano based systems.

Author Response

Ref: electrochem-2355971

 

Title: Bio-Medical Aspects of Nanoparticles

 

1. Reply to Reviewer #1’s comments:

 

I have many issues with this manuscript and do not recommend its publication.

Reply: Thank you for the critical feedback. We have suitably incorporated all the suggestions and corrections in the revised manuscript, making it suitable for publication.

1. The stated goal (l. 87-90 p.3) is way too ambitious and impossible to achieve in a single review. Also, that stated goal does not fit the article title.

Reply: Thanks for your critical comment. We agree with your point. The introduction line has been changed accordingly “The main aim of this review is to primarily provide the reader, a historic perspective of NM's (initiated from 1900s), their applications (supported in Figure 2) to the interdisciplinary field of biology, chemistry, electronics, and medicine. Secondly, to overview the most recent developments in this field (Figure 3) and finally discuss the hard road to commercialization (Section 5)”. The title suggested for the article is “Biomedical Aspects of Nanoparticle” This title is aptly chosen based on the timeline explained in the introduction, their applications, preparation and properties, characterization, applications and finally final product based in the field of Bio-medics.

We can incorporate the suggestions provided by the reviewer in the title.

2. In my view, the authors keep dispensing “information” in every direction without any transition. It makes the article difficult to read. It also makes it difficult to learn something from reading it.

 

Here is an example (l. 617-621 p. 17):

AuNP’s have same material to that of old jewelry, but the various shapes of AuNP allows various colors compared to yellow for bulk gold, due to interaction of light and further reflectance.

Here the authors seem to discuss the surface plasmon resonance properties of gold nanoparticles, which depend on nanoparticle size, shape, and surface adsorbents. I don’t see how the reader can understand what they are saying without already having the knowledge.

These different colors can be employed as biomarkers, fluorescent inducers for diagnosis of heath-related conditions.

Surface functionalization can be used to detect analytes. The latter involves either nanoparticle growth or aggregation, which affects the nanoparticle optical properties. Again, I don’t see how the reader can understand it from this writing.

Many metal based systems such as copper which is extremely rigid at nano level can be a ductile wire in bulk metal.

What does this mean and how does it relate to the previous sentence?

 

Reply: Thanks for the critical comment. We agree with your point. There were a few typographical errors and missing words. As suggested, the paragraph has been rewritten in the revised version of the manuscript.

3. The cartoonish figures are of poor quality.

Reply: Thanks for your comment. Just Figure 1 is a cartoonish figure. The rest of other Figures are Mind maps. The figures are made to keep the readers engaged into the research article and not make it monotonous for a review article reading.

 

4. Not all of the “information” dispensed in the article is properly referred to.

 

Here is an example (l. 167-179 p.5):

Precisely choosing of nanomaterials as a promising drug delivery platform that can help in altering the hydrophobicity, thereby enhancing the water-solubility and upgrading their bio-availability characteristics. It is acquired under the passive or active targeting of drugs based tailored systems that are quite responsive for stimuli such as optical, thermal and hydrogen ion concentrations changes. Owing to minute size-exclusion, they infiltrate through the capillaries to the targeted tumor sites where they accumulate and helps in decreasing/reduction in the after-effects i.e. mainly side effect and toxicity issues along with accelerating their efficacy. Representing features of controlled release, prolonged action of work, balance in drug levels and balancing the therapeutic window, thereby avoiding hazardous drug concentration in the organs. The choice of co-delivery of more than one for simultaneous therapy and diagnosis,[40,41] cumulatively incorporating traits of synergistic effects, tuning relative therapeutic regime, drug resistance, etc. is possible using nano based systems.

 

Reply: Thanks for your comment. This paragraph is a part under the subheading of “Healthcare”. The references in support are suitably added in places. A few sentences are incorporated to enhance the continuity of the article in the revised version of the manuscript.

Reviewer 2 Report

electrochem-2355971

Review of the review article titled:

«Bio-Medical Aspects of Nanoparticles»

 

by MANSI GANDHI, Khairunnisa Amreen

 

In Electrochem (ISSN 2673-3293).

 

Round 1

 

The abstract perfectly underlines the relevance of the presented studies.

 

The article was presented in a well-structured manner, with a good level of organization. Unfortunately, several statements within have weak evidence. Therefore, the referee suggested improving the manuscript with a major revision. The following is a list of specific concerns.

 

1.         Introduction. In the introduction section, it is necessary to clearly define the range of issues considered in the review and the time period. Here it is also required to note whether there are reviews on similar topics in world literature of the last ten years. List them and indicate the principal differences between this review and other available ones. -         The purpose of the Introduction is to attract the reader of Electrochem (MDPI) by what he can find here in contrast to similar publications.

2.         Please specify and add several statements in the main manuscript body concerning the study's aim and the novelty statement's key point. It seems to be odd to write another review as previously published.

3.         Adding a new section concerning materials' purity and stability is highly recommended.

4.         I think Figure 2 has no critical information.

5.         The presented review does not seem to include recent achievements and advances in this area. There are only 11 works cited here since 2019 (including) 100 in the review reference list. 2023 — 0; 2022 — 0; 2021 — 3, 2020 — 2, 2019 — 8.

6.         I think the review article should include much more than 250 cited works, including a time period of no more ten years.

Author Response

Ref: electrochem-2355971

 

Title: Bio-Medical Aspects of Nanoparticles

 

1. Reply to the Reviewer #2’s comments:

 

The abstract perfectly underlines the relevance of the presented studies. The article was presented in a well-structured manner, with a good level of organization. Unfortunately, several statements within have weak evidence. Therefore, the referee suggested improving the manuscript with a major revision. The following is a list of specific concerns.

Reply: Thanks for your positive feedback. We agree with your points. Changes have been made in the revised manuscript with additional evidences as suggested.

1. Introduction. In the introduction section, it is necessary to clearly define the range of issues considered in the review and the time period.

Reply: Thanks for your critical comment. The range of issues/topics is defined in the last few lines of introduction (Line 85 to 90).

Here it is also required to note whether there are reviews on similar topics in world literature of the last ten years. List them and indicate the principal differences between this review and other available ones. -The purpose of the Introduction is to attract the reader of Electrochem (MDPI) by what he can find here in contrast to similar publications.

There are a number of articles published on ‘nanomaterials’, ‘biomedical systems’, ‘metal based biomaterials in biomedical systems’, ‘controllable synthesis and biomedical applications of silver nanomaterials’, ‘advanced engineered nanomaterials for point-of-care immunosensing’ and so on and so forth. But a holistic research review from the starting of nanomaterials, their characterization techniques, characteristic properties along with their application sin domain of biomedical with respect to its characterizations and properties, followed by their limitations and advantages and finally their real-time applications used in industry products have not been published yet. This holistic approach makes this review article special and standout compared to other articles. A small table comparing 20 articles published and the principal difference is as follows.

S.No.	Article Title	Article Emphasis	DOI
1.	Self-Assembled Fluorescent Organic Nanomaterials for Biomedical Imaging	Only relevant to self-assembled fluorescent contrast agents for biomedical imaging.	10.1002/adhm.201800344
2.	Conducting Polymer Nanomaterials for Biomedical Applications: Cellular Interfacing and Biosensing	Only regarding conducting polymers and their biomedical applications	10.1080/15583724.2013.805771
3.	Recent advances in biomedical applications of 2D nanomaterials with peroxidase-like properties	Classification of different types of 2D POD-like nanomaterials, and their properties for biomedical applications	10.1016/j.addr.2022.114269
4.	The Advances and Biomedical Applications of Imageable Nanomaterials	Emphasised on the characteristics of imageable nanomaterials, highlighted the advantages and biomedical applications of imageable nanomaterials in the diagnosis and treatment of diseases	10.3389/fbioe.2022.914105
5.	Role of Nanomaterials in the Fabrication of bioNEMS/MEMS for Biomedical Applications and towards Pioneering Food Waste Utilisation	his review aspires to communicate recent progress in organic and inorganic nanomaterials based bioNEMS/MEMS for biomedical applications, comprehensively discussing nanomaterials criteria and their prospects as ideal tools for biomedical devices.	10.3390/nano12224025
6.	Controllable Synthesis and Biomedical Applications of Silver Nanomaterials	controllable synthesis of silver nanomaterials including nanorods, nanowires, nanotubes, nanoprisms, nanoplates, nanodisks, nanospheres, and nanopolyhedrons, etc. are reviewed. Their shape specific properties like antibacterial and antiviral applications, antitumor applications, biosensors and biological labels, optical imaging and imaging intensifier, are discussed.	10.1166/jnn.2011.5317
7.	Graphene and Graphene-Based Materials in Biomedical Applications	strategies to synthesize graphene and its derivatives are mentioned. Biocompatibility profile of synthesised graphene nanomaterials as a precursor to their biomedical application are discussed.	10.2174/0929867326666190705155854
8.	Peptide-Engineered Fluorescent Nanomaterials: Structure Design, Function Tailoring, and Biomedical Applications	Describes potential strategies for the preparation of peptide-engineered fluorescent nanomaterials functionalization and biomodification strategies for biomedical applications.	10.1002/smll.202005578
9.	Nanomaterials based on thermosensitive polymer in biomedical field	research progress of thermosensitive nanomaterials as "intelligent " systems for biomedical applications.	10.3389/fchem.2022.946183
10.	Research trends in biomedical applications of two-dimensional nanomaterials over the last decade-A bibliometric analysis	the contributions of 2D nanomaterials to the various biomedical subfields are highlighted	10.1016/j.addr.2022.114420
11.	Recent Advances of Cell Membrane-Coated Nanomaterials for Biomedical Applications	cell membrane-coated nanomaterials are reviewed based on the main biological functions of the cell membrane in living cells and their perspectives of biomedical applications are summarised.	10.1002/adfm.202003559
12.	Multifaceted Biomedical Applications of Functional Graphene Nanomaterials to Coated Substrates, Patterned Arrays and Hybrid Scaffolds	biological potentials of functional graphene nanomaterials and summarize some of major literature concerning the multifaceted biomedical applications	10.3390/nano7110369
13.	Biomedical Effects and Nanosafety of Engineered Nanomaterials: Recent Progress	recent advance in the research on the biological effects of engineered nanomaterials and nanosafety issue, by focusing on the studies about representative nanomaterials	10.1002/cjoc.201200662
14.	Preparation, applications, and challenges of functional DNA nanomaterials	reparation methods of DNA nanomaterials with functions such as molecular recognition, nanozyme catalysis, drug delivery, and biomedical material templates	10.1007/s12274-022-4793-5
15.	Aptamer functionalized nanomaterials for biomedical applications: Recent advances and new horizons	versatile roles of nanomaterials in advanced aptamer functionalized nanomaterials are discussed, including optical indicators, conducting transducer, carriers, catalysts, and other functions.	10.1016/j.nantod.2021.101177
16.	Current Use of Carbon-Based Materials for Biomedical Applications-A Prospective and Review	recent studies in developing carbon-based nanomaterials and its applications in targeting drug delivery, cancer therapy, and biosensors are discussed.	10.3390/pr8030355
17.	Layered double hydroxide-based nanomaterials for biomedical applications	Layered double hydroxides based nanomaterials with versatile physicochemical advantages for biomedical applications are summarised	10.1039/d2cs00236a
18.	Microfluidic Synthesis of Nanomaterials for Biomedical Applications	design principles of microfluidic systems for nanomaterials synthesis is summarized. Following, microfluidic platforms are extended as new avenues for custom-made complex nanomaterials for biomedical applications	10.1002/smtd.201700140
19.	Nanomaterial integrated 3D printing for biomedical applications	nanomaterials have been incorporated into 3D printing polymers to fabricate innovative, versatile, multifunctional hybrid materials. The review focuses on recent advances in novel hybrid biomaterials composed of nanomaterials and 3D printing technologies for biomedical applications	10.1039/d2tb00931e
20.	The usage of composite nanomaterials in biomedical engineering applications	In this review, overview of the development of biosensors for the diagnosis of diseases, drug targeting and controlled release applications, medical implants and imaging techniques are the research topics of nanobiotechnology.	10.1002/bit.27843

2. Please specify and add several statements in the main manuscript body concerning the study's aim and the novelty statement's key point. It seems to be odd to write another review as previously published.

Reply: Thanks for your critical comment. We agree highlighted the relevance of our study in the revised version of manuscript (Line 90 – 99; 143 – ).

3. Adding a new section concerning materials' purity and stability is highly recommended.

Reply: Thanks for your feedback. We have incorporated as a small part in the revised version of manuscript. (Line 428 – 442).

4. I think Figure 2 has no critical information.

Reply: Thanks for your comment. The Figure 2 is emphasis the introductory part for various fields of applications in context to nanomaterials.

 

5.The presented review does not seem to include recent achievements and advances in this area. There are only 11 works cited here since 2019 (including) 100 in the review reference list. 2023 — 0; 2022 — 0; 2021 — 3, 2020 — 2, 2019 — 8.

Reply: Thanks for your comment. The new updated reference list consists of 2023-2; 2022-5; 2021-4; 2020-3; 2019-8.

6. I think the review article should include much more than 250 cited works, including a time period of no more ten years.

Reply: Thanks for your critical comment. We do partially agree with your point. But the readers should have a background from its important references. They account for various important references that needs to bridge the roadmap for current achievement in the domain of biomedical systems using nanoparticles.

 

 

Author Response File: Author Response.pdf

Reviewer 3 Report

The authors presented the paper "Bio-Medical Aspects of Nanoparticles"

1) The reference list should be improved. Many more 2-3 years review papers should be cited in the Introduction section to show the progress in the area. There are only 16 references in the Introduction section. I highly recommend not using references older than 10 years for all sections, except for historically important works.

Maybe these works are suitable for the Introduction and other sections. You may also search in, for example, the MDPI system.

https://www.mdpi.com/2079-4991/13/3/574

https://www.mdpi.com/2312-7481/9/1/12

https://www.mdpi.com/2312-7481/8/10/131

https://www.mdpi.com/2312-7481/8/9/107

https://www.mdpi.com/2079-4991/12/16/2826

https://www.mdpi.com/2312-7481/8/2/13

https://www.mdpi.com/1999-4923/15/4/1104

https://www.mdpi.com/1999-4923/15/1/236

2) If the aim of the work presents Bio-Medical Aspects of Nanoparticles, please delete the non-biomedical applications such as wall painting, electronics, etc. elsewhere in the text. For example, Figure 2 and nearby text.

Figure 2. I recommend writing what you mean about medicine as imaging, drug delivery, etc., or dividing it into several areas.

Moreover, the magnetic separation of various compounds is an important area.

3) Figure 3, Section 3 I highly recommend presenting magnetic nanoparticles, bioinspared nanoparticles with fully biocompatible components such as albumin nanoparticles, CaCO3 nanoparticles, etc. 

Carbon is not so common nanoparticle for biomedical applications and they are inorganic too.

4) Section 5. I recommend dividing it into subsections. Please add drug and gene delivery, imaging, magnetic separation, etc. sections. Moreover, I recommend presenting some papers about doxorubicin and other drug-loaded nanocomposites.

For example,

https://www.mdpi.com/search?sort=pubdate&page_count=50&q=doxorubicin+magnetic+nanoparticles&year_from=2022&year_to=2023&featured=&subjects=chem-materials%2Cbio-life&journals=pharmaceutics%2Cnanomaterials%2Cpolymers%2Cmagnetochemistry%2Cijms&article_types=&countries=

https://www.mdpi.com/2079-4991/12/20/3686

https://www.mdpi.com/2312-7481/8/5/54

https://www.mdpi.com/1999-4923/15/1/292

https://www.mdpi.com/2079-4991/12/3/303

Moderate editing of English language

Author Response

Ref: electrochem-2355971

 

Title: Bio-Medical Aspects of Nanoparticles

 

1. Reply to the Reviewer #3’s comments:

The authors presented the paper "Bio-Medical Aspects of Nanoparticles"

1. The reference list should be improved. Many more 2-3 years review papers should be cited in the Introduction section to show the progress in the area. There are only 16 references in the Introduction section. I highly recommend not using references older than 10 years for all sections, except for historically important works.

Maybe these works are suitable for the Introduction and other sections. You may also search in, for example, the MDPI system.

https://www.mdpi.com/2079-4991/13/3/574

https://www.mdpi.com/2312-7481/9/1/12

https://www.mdpi.com/2312-7481/8/10/131

https://www.mdpi.com/2312-7481/8/9/107

https://www.mdpi.com/2079-4991/12/16/2826

https://www.mdpi.com/2312-7481/8/2/13

https://www.mdpi.com/1999-4923/15/4/1104

https://www.mdpi.com/1999-4923/15/1/236

Reply: Thanks for your comment. We have added a few lasted new references as suggested.

2) If the aim of the work presents Bio-Medical Aspects of Nanoparticles, please delete the non-biomedical applications such as wall painting, electronics, etc. elsewhere in the text. For example, Figure 2 and nearby text.

Figure 2. I recommend writing what you mean about medicine as imaging, drug delivery, etc., or dividing it into several areas.

Moreover, the magnetic separation of various compounds is an important area.

Reply: Thanks for your critical comment. Figure 1 and Figure 2 are part of the introductory section. Hence, the introduction part consists of a holistic background for the readers to engage in the article. A new Figure 3 is added in this respect. We have already incorporated imaging and drug delivery in the text in the later sections of the manuscript.

3) Figure 3, Section 3 I highly recommend presenting magnetic nanoparticles, bioinspared nanoparticles with fully biocompatible components such as albumin nanoparticles, CaCO3 nanoparticles, etc. 

Carbon is not so common nanoparticle for biomedical applications and they are inorganic too.

Reply: Thanks for your feedback. We agree with your point and we have suitably added it as Figure 2 with the write-up in Lines 173-200.

4) Section 5. I recommend dividing it into subsections. Please add drug and gene delivery, imaging, magnetic separation, etc. sections. Moreover, I recommend presenting some papers about doxorubicin and other drug-loaded nanocomposites.

For example,

https://www.mdpi.com/search?sort=pubdate&page_count=50&q=doxorubicin+magnetic+nanoparticles&year_from=2022&year_to=2023&featured=&subjects=chem-materials%2Cbio-life&journals=pharmaceutics%2Cnanomaterials%2Cpolymers%2Cmagnetochemistry%2Cijms&article_types=&countries=

https://www.mdpi.com/2079-4991/12/20/3686

https://www.mdpi.com/2312-7481/8/5/54

https://www.mdpi.com/1999-4923/15/1/292

https://www.mdpi.com/2079-4991/12/3/303

Reply: Thanks for your feedback. We agree with your point. Lines 200-385 highlight the use of nanoparticles in drug delivery. We have suitably incorporated a few references as suggested.

Round 2

Reviewer 2 Report

The authors addressed my comments well. I would recommend its acceptance.

Author Response

Thankyou for your valuable comments. Thank you for your positive feedback.