Evaluation of Antidiabetic Potential of Mangifera indica Leaf in Streptozotocin-Induced Type 2 Diabetic Rats: Focus on Glycemic Control and Cholesterol Regulation
, Anika E. Richi 1, Sanjida A. Riju 1, Tanjila Jalal 1, Rejwana J. Orchi 1, Smita Singh 2, Phulgen Bhagat 2, Yasser H. A. Abdel-Wahab 3 and Prawej Ansari 1,2,3,*Round 1
Reviewer 1 Report
Comments and Suggestions for Authors
The authors have made a very nice work. Very precise and very nice organized. In their work they investigated the insulin-releasing and glucose-lowering potential of the ethanolic extract of Mangifera indica (EEMI) leaves in streptozotocin (STZ)-induced type 2 diabetic rats, concurrently investigating its phytoconstituents.
Howvever,
there are missing some important parts of the study, so please be kind to provide the explanations:
The main comments are:
In the methods part should be mentioned the ethical board approval for using laboratory animals (number of approval).
How was the blood taken form the vein? Have the animals been at sleep or awake? Please mention the mechanism, the anesthesia, etc. These explanations includes administration of streptozotocin i.p. as well.
There is missing the control animals that get the saline intraperitoneally.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for Authors
The manuscript titled "Evaluation of Antidiabetic Potential of Mangifera indica Leaf in Streptozotocin-Induced Type 2 Diabetic Rats: A Focus on Glycaemic Control and Cholesterol Regulation" explores the antidiabetic effects of Mangifera indica (mango) leaf extract in diabetic rats. The study is well-structured, offering valuable insights into the potential use of mango leaves in managing type 2 diabetes through various in vitro and in vivo experiments. I recommend subject to the completion of major revisions in line with the comments provided below.
Comments to the Authors:
- Introduction, page 2: You wrote "….. inhibiting intestinal glucose absorption through α-amylase and α-glucosidase inhibition [8,9]". α-Amylase primarily functions in the breakdown of starches into smaller carbohydrate molecules, which then can be further broken down into glucose by other enzymes like α-glucosidase. The inhibition of α-amylase and α-glucosidase contributes to a reduction in glucose production from dietary starches, rather than directly inhibiting the absorption of glucose in the intestine. The sentence should be corrected.
- Introduction, pages 1-2: The introduction provides a comprehensive background on diabetes and the potential of medicinal plants in its treatment. However, it could be enhanced by briefly discussing previous studies specifically related to Mangifera indica's antidiabetic effects to contextualize scientific work within existing research. Search on PubMed with the keywords " antidiabetic Mangifera indica" indeed reveals a significant amount of research, with over 70 published papers on the topic. This indicates a robust interest and investigation into the antidiabetic properties of Mangifera indica. The studies likely cover various aspects, including the mechanisms of action, efficacy in different models, and potentially the identification of bioactive compounds responsible for the antidiabetic effects. In the introduction section, only a limited number of studies concerning the antidiabetic effects of Mangifera indica are referenced.
- Materials and Methods, pages 2-5: The methods section is detailed, covering plant collection, extract preparation, and various assays conducted. It should include more information on the ethical considerations and approvals for animal experiments to ensure the study's compliance with ethical standards.
- Results, pages 5-12: The results are presented clearly, showing the positive effects of EEMI on insulin release, glucose tolerance, and lipid profiles. Including comparative data or a discussion on how these results align or differ from existing studies on similar extracts or antidiabetic treatments could provide a deeper insight into EEMI's unique contributions.
- Limitations and Future Directions: While the manuscript briefly mentions future research directions, explicitly stating the limitations of the current study would provide a more balanced view and set a clear path for subsequent research.
Comments on the Quality of English Language
No comments
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for Authors
Please see the attached file.
Comments for author File: Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for Authors
all issues addressed
Reviewer 2 Report
Comments and Suggestions for Authors
The manuscript was revised according to the previous comments and is recommended for acceptance in its present form
