Pulsatilla ×
hackelii Pohl. is the most common hybrid between
Pulsatilla patens and
Pulsatilla pratensis. It occurs in the natural environment, most often in sites together with parental forms.
Pulsatilla × hackelii is morphologically intermediate between the pure
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Pulsatilla ×
hackelii Pohl. is the most common hybrid between
Pulsatilla patens and
Pulsatilla pratensis. It occurs in the natural environment, most often in sites together with parental forms.
Pulsatilla × hackelii is morphologically intermediate between the pure
Pulsatilla species. Separation of hybrid individuals from pure
Pulsatilla species requires a whole set of morphological features. Completed plastid genome provides molecular evidence for the hybrid origin of
Pulsatilla × hackelii. In our study, we present the—genotypic characteristics of
Pulsatilla ×
hackelii based on microsatellite markers developed for
Pulsatilla vulgaris. A specimen of the hybrid was found in the Świętokrzyskie Mountains, near Bocheniec village, in the vicinity of a forest road in a fresh mixed coniferous forest (
Querco roboris-Pinetum (W. Mat. 1981) J. Mat. 1988). We amplified a set of 10 microsatellites in two multiplex PCR reactions. From the 10 analyzed loci, we successfully amplified eight. The analyzed microsatellites in population studies of three
Pulsatilla species showed some species-specific alleles. In the case of the
Pulsatilla ×
hackelii sample, we obtained PCR products in the case of 5 loci developed for
P. vulgaris but successfully crossamplified in P.
patens and
P. pratensis. Additionally, we obtained results for two loci, which crossamplified only for
Pulsatilla patens and one only for
Pulsatilla pratensis. Among the investigated markers, two were monomorphic and the remaining six were polymorphic, which makes them highly informative in population studies for pasque-flower species as
Pulsatilla patens,
P. pratensis, and
P. vernalis. The obtained results indicated that analyzed microsatellite markers could be used for hybrids identification, although there is a need for further research on a larger number of hybridized individuals.
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