J. Zool. Bot. Gard., Volume 3, Issue 2 (June 2022) – 12 articles

Zoos strive to create experiences that inspire positive feelings toward animals which lead to conservation behaviors in their visitors. However, concerns regarding the welfare of animals living in zoos present a challenge in creating positive zoo experiences and promoting the conservation agenda and moral authority of these cultural institutions. This research explores connections between zoo visitors’ positive affective responses and their assessments of animal welfare before and after two giraffes were introduced to a group of four giraffes in a multi-species savannah exhibit. A self administered questionnaire was completed by 499 visitors to the Conservation Society of California’s Oakland Zoo. The questionnaire measured visitors’ predispositions, affective responses, and assessments of animal welfare. Results suggest that visitors’ assessments of animal welfare, positive affective experience, and predisposition are positively correlated. Further, visitor assessments of animal welfare are generally more positive after the addition of new giraffes. Although visitors tended to report that the giraffes were very healthy and well cared for, they responded less positively when asked about how happy the giraffes were and how adequately sized their exhibit was. The findings suggest that understanding and improving zoo visitors’ assessments of animal welfare is important in improving positive experiences and conservation education outcomes during a visit to the zoo. Full article

Zoos engaged in a range of communication types with prospective visitors during the temporary closures necessitated by the COVID-19 pandemic. This study sought to (1) investigate social media reports and public responses to zoo-animal-related posts over a one-year period during COVID-19 lockdowns; (2) understand the use of reporting language in news articles concerning animal responses during zoo closures, and to investigate whether this differed across species; and (3) investigate how keepers perceived general animal behavior, and how they perceived animal behavior in keeper–animal interactions, during the COVID-19 facility closures. Data were collected from BIAZA-accredited zoos’ Facebook pages (March 2020 to March 2021) and news reports (Google search outputs from 20 March to 5 April 2021). Keeper perceptions were captured via questionnaires (May to August 2021). Data were collected on taxa, the reported behavioral changes and the language used in media communications. In Facebook posts and news reports, mammals were more frequently represented than was expected (p < 0.05). Behavioral responses were more frequently negative (p < 0.05) and less frequently positive or neutral (p < 0.05). Keepers reported overall behavioral changes, as well as changes during their own interactions with animals. On Facebook, mammals were described using a combination of behavioral descriptions and anthropomorphic terms, which were used more frequently than was expected (p < 0.05). In the news reports concerning primate species, anthropomorphic descriptions were used more frequently than expected (p < 0.05), while behavioral descriptions were used less frequently than expected (p < 0.05). The reports regarding the Carnivora were the reverse of this. This study enabled an understanding of the impact of the temporary closures on the animals, and how this impact was communicated to the public. The findings may reflect the relationships that humans have with animals and the need for communication methods that will capture visitors’ interest and induce empathy with the various species. Full article

Visitor effect studies have been of keen interest for decades, but there have been only anecdotal opportunities to investigate the impact of the prolonged absence of visitors on animal welfare in zoos. In some zoos, bears are actively involved in animal–visitors interaction through begging, which gives them some degree of control over gaining food rewards throughout the day when visitors are present. Prolonged visitor absence may, therefore, represent a loss of control and have a negative impact on the bears’ welfare. In this study, we investigate the behavior of four brown bears (Ursus arctos) in three zoos to see how the animals’ behavior changed depending on the presence and absence of the public during zoo closures in 2020 related to the COVID-19 pandemic. The bears’ behavior was assessed using activity budget analysis and novel object tests. The results showed that the bears behavior varied between facilities and that there was no effect of visitor presence for the individuals who had access to indoor holding space. The results of the study are discussed in terms of choice and control and their impact on the wellbeing of bears in zoos. Full article

The space use of captive animals has been reliably used as a tool to measure animal welfare in recent years. However, most analyses of space use focus primarily on terrestrial animals, with very little emphasis placed on the space use of aquatic animals. By comparing the space use of these animals to their natural histories and what would be expected of them physiologically, a general assessment of their overall welfare can be obtained. Using the Zoomonitor program, this study investigated the space use of five elasmobranch species housed in a captive aquatic environment: a blacktip reef shark (Carcharhinus melanopterus), a nurse shark (Ginglymostoma cirratum), a smooth dogfish (Musteluscanis), a bonnethead shark (Sphyrna tiburo), and a blacknose shark (Carcharhinus acronotus). The exhibit was delineated into five different zones: three represented the animal locations along the X/Y axis (‘Exhibit Use’), and two zones were related to the Z-axis (‘Depth Use’). The location of each individual on both the X/Y and Z axes was recorded during each observation. Heat maps generated from the Zoomonitor program were used in conjunction with the Spread of Participation Index (SPI) to interpret the data. It was found that while all the individuals used their given space differently, the Exhibit Use was relatively even overall (the SPI values ranged from 0.0378 to 0.367), while the Depth Use was more uneven (the SPI ranged from 0.679 to 0.922). These results mostly reflected what would be expected based on the species’ natural histories. However, for the smooth dogfish, the observed Exhibit Use and activity patterns revealed a mismatch between the anticipated and the actual results, leading to further interventions. As demonstrated here, space use results can be utilized to make positive changes to husbandry routines and enclosure designs for aquatic individuals; they are thus an important additional welfare measure to consider for aquatic species. Full article

Pangolins are under severe threat of surging poaching rates globally; therefore, there is a demand to ascertain reproductive measures to ensure captive breeding and management strategies. Due to the absence of substantial information on the pangolin, endocrinology and reproductive physiology studies around the globe are merely based on captive observations that have failed to report the chronographs and hormonal cyclicity of the reproductive events. This study attempts to evaluate the annual pattern of reproductive steroids (estradiol-17β and progesterone) and prolactin in 16 wild female Taiwanese pangolins rehabilitated by Pingtung Rescue Center of Endangered Wild Animals, Taiwan. Novel immunoassays, i.e., chemiluminometric assays, have been used to quantify the serum reproductive steroids and contribute to a better understanding of the endocrine correlates of function in the Taiwanese pangolin. The hematological findings were characterized by monthly median concentration. The circulating reproductive hormones demonstrated seasonal reproductive activity by confirming a peak in serum estradiol concentrations in December and considerably higher progesterone concentrations in November/December, and March/April. The rise in prolactin in December and peak values in April suggest participation in the ovulatory process and mating. Collectively, these findings can help maximize the reproductive efficiency of pangolin species in captivity, i.e., by timely pairing and prioritizing the care of the breeding pairs to optimize breeding efforts and, therefore, effectively support conservation breeding programs and restore the natural population in the ecosystems. Full article

The red panda is listed as “endangered” in the IUCN Red List of Threatened Species, due to the rapid population decline. Improving our knowledge on the red panda biology and ethology is necessary to enhance its husbandry and breeding in zoos. Behavioural variety, intended as the presence of a wide array of species-specific behaviour, has been considered a positive welfare index in zoo-housed animals. The aim of this study was to describe the behaviour of two pairs of zoo-housed red pandas, one of them with an offspring, and to investigate the behavioural variability using the Behavioural Variety Index (BVI). Behavioural data from two zoo-living male–female pairs were collected. A continuous focal animal sampling method was used to collect individual and social behaviours of the two pairs. Forty-eight 30 min sessions per subject were carried out. For the BVI, a list of species-specific behaviours previously reported in the red panda was prepared and compared with the behavioural repertoire of the subjects of the study. First, species-specific behaviours were recorded, and no abnormal behaviour was reported. The percentages of time spent on different activities (e.g., routine behaviours, exploratory/territorial behaviours, consumption behaviours, locomotive behaviours, social behaviours, maternal behaviours) were similar to time budgets reported in the red panda, with routine behaviours (resting, comfort and vigilance) being the most performed in both pairs. Moreover, the BVI suggested that each red panda performed on average 73% of the behaviours described in previous literature on this species. In conclusion, studying the behavioural variety of red pandas in zoos can be a useful tool for assessing their welfare as well as improving our knowledge on the behavioural repertoire of a species that is difficult to observe in the wild. Full article

Animal welfare is a priority across accredited zoological institutions; however, historically, research has been prioritized for mammals. Bird-focused studies accounted for less than 10% of welfare research in zoos and aquariums over the last ten years. Due to the lack of scientific publications on bird welfare, zoo scientists and animal practitioners can look to other industries such as agriculture, laboratories, and companion animal research for insight. This qualitative review highlights findings across industries to inform animal care staff and scientists on the welfare needs of birds within zoos and aquariums. Specifically, the review includes an overview of research on different topics and a summary of key findings across nine resources that affect bird welfare. We also highlight areas where additional research is necessary. Future welfare research in zoos and aquariums should prioritize studies that consider a diversity of bird species across topics and work to identify animal-based measures with empirical evidence. Moving forward, research from other industries can help develop innovative research on bird welfare within zoos and aquariums. Full article

Animal behavior and welfare science can form the basis of zoo animal management. However, even basic behavioral data are lacking for the majority of amphibian species, and species-specific research is required to inform management. Our goal was to develop the first ethogram for the critically endangered frog Xenopus longipes through observation of a captive population of 24 frogs. The ethogram was applied to produce a diurnal activity budget and to measure the behavioral impact of a routine health check where frogs were restrained. In the activity budget, frogs spent the vast majority of time swimming, resting in small amounts of time devoted to feeding, foraging, breathing, and (in males) amplexus. Using linear mixed models, we found no effect of time of day or sex on baseline behavior, other than for breathing, which had a greater duration in females. Linear mixed models indicated significant effects of the health check on duration of swimming, resting, foraging, feeding, and breathing behaviors for all frogs. This indicates a welfare trade-off associated with veterinary monitoring and highlights the importance of non-invasive monitoring where possible, as well as providing candidates for behavioral monitoring of acute stress. This investigation has provided the first behavioral data for this species which can be applied to future research regarding husbandry and management practices. Full article

Many birds, including macaws, are highly visual animals able to detect a wide band of light wavelengths ranging into ultraviolet A, but in captivity, full-spectrum lighting is not universally employed. Where purpose-made bird lighting is used, this is typically made with the provision of ultraviolet B radiation and vitamin D₃ synthesis in mind. Limited research in this field suggests behavioural and physiological benefits of broad-spectrum lighting provision, but more work is needed to broaden the taxonomic scope and to investigate its impacts on understudied areas of husbandry, including behavioural management. We compared the duration of time a bonded pair of blue-throated macaws at ZSL London Zoo opted to remain in an inside den after being recalled from an outdoors flight aviary, with and without the presence of artificial lighting in the form of High Output T5 Fluorescent lamps, which are rich in UVA and UVB wavelengths as well as those visible to humans. We hypothesized that the birds would remain inside for longer when T5 lighting was on, as they would be more visually comfortable. Using randomization analyses, we show that, over 54 trials split between winter and spring, the mean duration spent inside after recall increased from 81.04 to 515.13 s with the presence of the lighting unit, which was highly statistically significant. Our results are likely to be explained by much higher visibility of indoor surroundings creating a more hospitable indoor environment for the birds and will have implications for captive macaw management. Full article

The Botanic Garden of Pisa was established in 1543 as a teaching tool and research facility. As with the vast majority of its sister institutions, it focuses on plant collections. However, for a short time in the first half of the XIX century, the Botanic Garden exhibited a living giraffe, a cow, and a calf. Due to the transient nature of the exhibition, it could have easily gone unnoticed but for the fortuitous representation of the animals in a drawing of the same period and for sparse notes archived in the libraries of Pisa University. Furthermore, a XIX-century publication on the morphological and behavioural traits of three antelopes indirectly suggests that those animals had been kept in the Botanic Garden for research purposes. This paper presents the evidence of the living animal display in the Botanic Garden of Pisa and the context in which it was collected. Full article

Zoos include invertebrates in visitor interaction sessions to educate and spread conservation messages to the public. Yet, the welfare implications of these encounters on invertebrates are unstudied. Empirical studies reveal negative effects of handling on vertebrate species, thus providing reason to investigate impacts on invertebrates. Mexican red-kneed spiders Brachypelma hamorii are regularly handled by keepers for public talks at the Zoological Society of London, London Zoo. This study investigates whether handling affects the spiders’ 24-h activity and enclosure usage. Three spiders were filmed under infrared light for 24 h following being handled, and on control (no-handling) days. The proportion of time that spiders spent under cover or exhibiting locomotion, limb-interaction, and object-interaction behaviour was recorded using instantaneous scan sampling. The spiders spent, on average, significantly more time under cover (7.8% increase) and exhibited significantly more limb-interaction behaviour (1.4% increase) on handling days. Handling for public talks therefore affects the behaviour and enclosure use of these captive Brachypelma hamorii. Although it is not yet possible to infer welfare implications, the presence of these behavioral responses suggests that protocols to monitor the behaviour of invertebrates following handling should be developed, and further behavioral studies are warranted to validate potential stress indices. Full article

It is well known that captive animals alter their behavior and space use when observed by visitors, with the concept coined the ‘visitor effect’. The ‘observer effect’, described as any alteration in behavior and enclosure use as a result of a quiet, stationary observer, has been less studied. This study investigates the observer effect in two pairs of Edwards’ pheasants (Lophura edwardsi) and their offspring at Sparsholt College, United Kingdom. The impact of an observer (as opposed to camera) on behavior and enclosure use of pheasants was observed, using instantaneous focal sampling. Enclosure use was measured by converting both enclosures into unequal zones and then assessing the evenness of enclosure use through modified Spread of Participation Index. Poisson regression analysis was used to investigate observer impact, alongside the additional variables of keeper and visitor presence, temperature, and individual bird differences. Overall, the behaviors of resting and clustering were significantly increased during observer presence, whereas feeding and locomotion were significantly decreased. The behaviors of preening and standing were not affected by observer presence, though they were influenced by keeper and visitor presence. Enclosure use was also affected by observer presence, though the effect size was small. This suggests that pheasants may perceive the presence of humans near their enclosures as a potential threat, and may alter their behavior to reduce detection, similar to their wild counterparts. Animal researchers should consider the potential impact of observer presence on their subjects, particularly when observing species such as pheasants. Full article