Forensic Sci., Volume 1, Issue 3 (December 2021) – 11 articles

Documented skeletal collections are the backbone of forensic anthropology due to their associated biohistories. This paper describes the identified skeletal collections and their relevance in forensic anthropological research, education and training in the US. The establishment of documented skeletal collections in the US can be distinguished into two modus operandi, depending on the stance towards the dead, legislation, and medical and forensic practices. In the 19th and early 20th centuries, anatomists amassed skeletons from cadaver dissections, shaped by European influences. Those skeletons compose the anatomical collections—such as the Robert J. Terry Anatomical Collection—predominantly representing impoverished and unclaimed individuals. Ethical concerns for the curation and research of African American skeletons without family consent are growing in the US. In contrast, since the 1980s, modern documented skeletal collections originated from body donations to human taphonomy facilities, such as the William M. Bass Donated Skeletal Collection. The establishment and testing of osteological methods essential to establish one’s identity—such as age at death and sex—have been developed with skeletons from documented collections. Therefore, the analysis of identified skeletons has been crucial for the development of forensic anthropology in the US. Full article

The Pretoria Bone Collection (PBC) began in 1942 with the opening of a medical school at the University of Pretoria (UP) in Pretoria, South Africa, where this skeletal collection is housed in the Department of Anatomy. The purpose of this paper is to provide information on the national legislation associated with obtaining, curating and researching skeletal remains in South Africa, the demographic composition of the PBC, and the inclusion of a portion of these remains into a digital repository known as Bakeng se Afrika (BsA). The PBC comprises 873 complete skeletons, 344 complete postcrania without crania, and 308 complete crania without postcrania. Skeletal contents are reflective of the population statistics of South Africa, with a smaller proportion of White (32%) than Black (65%) South Africans. Unlike the population profile, males in the PBC are greater in number (75.5%) than females (24.5%), which may be explained by the number of migrant labourers traveling into a large city such as Pretoria. From this sample, crania (206), maxillae (141), mandibulae (408), femora (137), and radii (134), as well as several other skeletal elements were micro-XCT scanned and are available on the BsA server. A researcher needs to submit an online application to the Human Research Ethics Committee (HREC) of the Faculty of Health Sciences at the University of Pretoria for access to these collections. Full article

The degree of ossification of the thyroid cartilage in anthropological studies is related to other methods of determining the age of a skeleton. The endochondral ossification process begins at the age of approximately 15–20 years and is generally completed in the fifth or sixth decade of life. In the present case, early and complete mineralization of the thyroid cartilage of a skeleton belonging to a 21-year-old young soldier who died in 1946 was observed. Ossified thyroid cartilage at a very young age is rare and may be associated with specific symptoms, such as compression due to trauma, or may also be related to hormonal dysfunction and various diseases that may cause early mineralization. A macroscopic morphological evaluation and radiographic examination of the thyroid cartilage were performed and a decalcification test was applied to a sample taken from the thyroid cartilage to confirm that the structure was indeed mineralized. There is nothing to exclude that this is simply a physiological anatomical variation. Knowledge of this rare anatomical abnormality at a young age would be useful for the diagnosis of various pathological conditions. Full article

Lightweight sonar devices may be tethered to an unmanned aerial vehicle or drone and quickly deployed over water for real-time imaging in 2D and the on site creation of geolocated, interactive bathymetric maps without the need for a boat. We show how such data is useful in the preliminary stages of water searches, by providing geophysicists, hydrologists and divers with spatial depth information, the distribution of dive and equipment hazards such as entanglement objects (weed, discarded items) and sediment types. One bathymetry case study location is described in detail, with a further two summarized to demonstrate reconnaissance surveys. Limitations of drone-based sonar surveys are outlined, including dense water weed cover; limits on flight times and adverse weather conditions. Full article

Bones and teeth are highly challenging sources of DNA in forensic science and human remains identification, requiring multiple laborious processing steps. In this study, we compared an organic phenol–chloroform method to the QIAamp^® DNA Investigator and PrepFiler Express BTA™ methods in order to identify the most efficient automated DNA extraction method for bones and teeth. Results from individual tooth powder replicates showed that the PrepFiler Express BTA™ method extracted the highest yields of DNA per mg of tooth powder, returning a minimum of 20/21 PowerPlex^® 21 loci. Samples extracted using the organic extraction or QIAamp^® DNA Investigator methods produced PowerPlex^® 21 profiles displaying a ski-slope morphology. The improved DNA quality and yield from the PrepFiler Express BTA™ method was verified using aged samples, where higher DNA yields per mg of powder and more informative profiles were obtained. Furthermore, the PrepFiler Express BTA™ method subsequently provided useful DNA profiles for two forensic cases involving degraded bone samples. Overall, this study showed that the PrepFiler Express BTA™ chemistry is a reliable and robust method for DNA extraction from bone and teeth samples, and will allow larger numbers of samples to be efficiently extracted in the event of a Disaster Victim Identification event. Full article

In recent years, halal certification has become an area of significant interest due to the high level of Muslim awareness about halal products, with the development of halal hubs and investments in Muslim countries. Unfortunately, the halal market is negatively affected by various factors, like the multiplicity of halal standards, disagreements between halal certification bodies and halal accreditation bodies, an increase in halal food crime in some countries, weakness of the authorities in ensuring the integrity of halal certification, and involvement with politics and religion, which have caused some anxiety among Muslim consumers. Therefore, the aim of this work is to inform lawmakers and animal welfare organisations in the EU regarding halal food. It shows data from literature regarding animal welfare in the EU and Islam, provides a critical consideration on the aim of a religious diet and its correlation with public health and food safety, and describes the state of the art of halal accreditation bodies (HAB) and halal certification bodies (HCB), in addition to providing information about gaps in the halal market, and a description of the unethical behaviour of some HCBs. The integrity of these bodies is necessary to make halal standards more comprehensive and efficient, and this can be achieved through the activation of a harmonised monitoring system for halal accreditation bodies in Muslim countries and national EU accreditation bodies. Full article

The purpose of photography in violent death autopsies is to document the material evidence of the offense and guide decisions in the courts of law. The aim of the present study was to obtain the expectations of legal operators and forensic experts regarding what is expected as adequate photographic documentation in violent death forensics. For this purpose, a survey was prepared through an online form available on the “Google Forms” platform, whose link was sent by e-mail and by the WhatsApp Messenger^® application. The 102 participants were divided into two distinct groups: legal practitioners, interested in the materialization of evidence (judges, prosecutors, criminal lawyers and police chiefs), and forensic experts, responsible for materializing the evidence (medical examiners, forensic dentists and criminal experts). The research showed that the inclusion of color photographs in the expert reports is essential for all research participants, as well as the marking of evidence in the images and the inclusion of explanatory text in the captions. It was also pointed out as fundamental for most participants to insert an image with simulation of the firearm bullets’ path, when applicable. In relation to the other aspects, it can be observed that the opinions of the participants were divergent between the groups of research professionals, especially regarding the size of the image to be incorporated in the reports. The differences found between the groups can be detrimental to the proper interpretation and judgment of evidence in the courts. Considering the found barriers, the authors suggest a form of photographic documentation that meets all expectations in a consensual way. Full article

Advances in forensic biology have increased the options for the collection, sampling, preservation and processing of human remains for DNA-based identification. Combined with a plethora of commercial DNA testing kits that are far more forgiving of inhibited and degraded samples, efficient DNA approaches to post-mortem samples are explored here for DNA-based identification of compromised human remains. Approaches which preserve sample and reduce analytical turnaround times whilst saving resources also have the potential to expedite the identification process, to provide answers to grieving families sooner, or to provide leads in a criminal investigation. Targeting sample types that are minimally-invasive and do not require extensive preparation and testing protocols also has benefit for disaster victim identification (DVI) by facilitating field sampling. We have assessed minimally-invasive and simple to collect sample types compatible with minimal pre-treatment and efficient DNA profiling approaches. Incubating nail, distal phalanges and whole digits in 500 µL of PrepFiler™ Lysis Buffer for 2 h was an efficient and simple method, limiting or removing sample preparation. A reduced 15 min incubation also yielded DNA profiles suggesting a shorter incubation may lyse sufficient DNA. Preservative solutions offer an even simpler process in some cases. Furthermore, the efficient approaches described in this study offer storage solutions and are compatible with backend automated processing. This study will inform further research to develop and optimise efficient protocols. These DNA approaches should not be pursued for every sample; more compromised samples may best be submitted to the laboratory for more effective extraction and genotyping. Full article

Forensic anthropological investigations often encounter cases that are difficult to interpret, especially when dealing with skeletal remains found in a marine context. Determining the immersion time in the sea of skeletal remains is a challenge for forensic investigations and answering this would solve many cases in the shortest time possible. The physical and chemical properties of bones change during their time in water and these changes cause difficulties and delays in identifying and reconstructing the biological profile of an unknown subject. In this paper, two forensic cases found at sea were analysed, the case of a disarticulated and extensively skeletonised corpse found on the coast of Reggio Calabria (Italy) and the case of an isolated foot, intact of soft tissues, found only 9 km away, on the coast of Vibo Valentia (Italy). The resolution hypothesis of the two cases was based on three search options because the disappearance of three known individuals was being investigated simultaneously. The investigations were conducted through a multidisciplinary work applying different analyses, including anthropometric, radiological, digital and, finally, genetic analyses. The results made it possible to determine the reconstruction of two biological profiles, both of Case A, the skeletal remain, and Case B, the subject to whom the foot belonged. The almost compatible anthropometric results of the two biological profiles, the presence of two very indicative partial tattoos and a genetic correlation led to the solution of a single court case. Full article

The aqueous search for objects covered by sediment is a common and challenging problem. Here we outline a sequential methodology for the assessment of targets identified by sub-bottom profiling. This comprises desktop study of available data; background hydrological information gathering (bathymetry, sediment cover, water chemistry); acoustic sub-bottom imaging (water-penetrating radar, sonar); geolocation and probing of sub-bottom anomalies; and deployment of suitable scent dogs. This procedure creates a hierarchy of targets for examination by dive teams and thence recovery. Full article

ABAcard^® HemaTrace^® kits have been used for crime scene stains for confirmation of human blood for many years. However, when the stain is too small to allow for separate testing, confirmatory testing may be forgone to preference DNA analysis. This can lead to court challenges as to the biological source and therefore probative value of the DNA profile. This research aimed to develop a protocol for DNA analysis of a minute blood stain subsequent to HemaTrace^® testing. Stains were collected and subjected to HemaTrace^® testing. Swabs were then removed from the HemaTrace^® buffer solution and processed. DNA yields and STR DNA profiles were analysed for both quantity and quality. Full profiles were reliably obtained from stains with diameters of 0.6 mm–0.7 mm, reflecting DNA concentrations between 0.0036 ng/μL and 0.007 ng/μL, varying according to substrate characteristics. However, stains below a diameter of 0.6 mm should proceed directly for DNA profiling. This protocol was also successfully performed on blood stains which had undergone UV irradiation, although use of the reporting peak height threshold (lower than the routine analytical threshold) was required to obtain useable profiles. We have been able to demonstrate a protocol which, with minor adjustments to crime scene procedures, allows for both the confirmation of the presence of human blood, together with the generation of useful DNA profiles. Full article