Primary Characterization of a Life-Cycle Mutant akasusabi of the Red Alga Neopyropia yezoensis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation of a Mutant
2.2. Algal Strain, Culture Conditions and Calculation of the Growth Rate
2.3. Quantitative Measurement of Photosynthetic Pigments
2.4. Stress Treatments
2.5. Statistics Analysis
3. Results
3.1. Increased Growth of aks Gametophytes
3.2. Sporophyte Production with Mitotic Defect in aks Carposporangia
3.3. High Spore Productivity after Wounding Stress in aks
3.4. Heat Stress Inducibility of Callus Formation in aks
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | Carposporangia 1 | Carpospores 2 | ||||||
---|---|---|---|---|---|---|---|---|
WT | Two-cell layer | Four-cell layer | ||||||
Upper | Lower | Top to bottom | ||||||
11.4 ± 2.6 (n = 14) | 9.61 ± 2.1 (n = 14) | 4.69 ± 0.6 (n = 7) | 4.65 ± 0.5 (n = 7) | 4.68 ± 0.4 (n = 7) | 5.23 ± 0.6 (n = 7) | 10.26 ± 1.7 (n = 62) | ||
aks | One-cell layer | Two-cell layer | ||||||
Upper | Lower | |||||||
17.5 ± 3.3 | 11.68 ± 1.6 | 11.18 ± 1.4 | 11.84 ± 1.4 | |||||
(n = 41) | (n = 30) | (n = 30) | (n = 76) |
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Mikami, K.; Matsumura, T.; Yamamoto, Y. Primary Characterization of a Life-Cycle Mutant akasusabi of the Red Alga Neopyropia yezoensis. Phycology 2021, 1, 14-26. https://doi.org/10.3390/phycology1010002
Mikami K, Matsumura T, Yamamoto Y. Primary Characterization of a Life-Cycle Mutant akasusabi of the Red Alga Neopyropia yezoensis. Phycology. 2021; 1(1):14-26. https://doi.org/10.3390/phycology1010002
Chicago/Turabian StyleMikami, Koji, Takaharu Matsumura, and Yuji Yamamoto. 2021. "Primary Characterization of a Life-Cycle Mutant akasusabi of the Red Alga Neopyropia yezoensis" Phycology 1, no. 1: 14-26. https://doi.org/10.3390/phycology1010002