Aurones as Antidiabetic Agents and Their Prebiotic Activities
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Extraction and Isolation
2.3. Assessment of Biological Activities
2.3.1. In Vitro Glycogen Phosphorylase Inhibition Assay
2.3.2. Inhibition of α-Amylase
2.3.3. Inhibition of α-Glucosidase
2.4. Prebiotic Activity
2.4.1. Strains
2.4.2. Prebiotic Activity Score (Pscore)
2.5. Docking Studies
2.5.1. Preparation of the Target
2.5.2. Docking of the Tested Molecules to the Enzyme Binding Site
2.6. Statistical Analysis
3. Results and Discussions
3.1. Identification of Isolated Compounds
3.2. Assessment of Antidiabetic Activity of the Isolated Compounds
3.3. Prebiotic Activity Score of the Isolated Compounds
3.4. Docking Studies
3.4.1. Docking Analysis of the Test Samples and Positive Control (Acarbose) against α-Amylase
3.4.2. Docking Analysis of α-Glucosidase
3.4.3. Docking Analysis of Glycogen Phosphorylase
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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% Inhibition | |||||
---|---|---|---|---|---|
α-Glucosidase | α-Amylase | GP | |||
Acarbose | 97.7 ± 0.54 | Acarbose | 97.7 ± 0.88 | CP-91149 | 84.9 ± 0.81 |
1 | 60.5 ± 1.54 | 1 | 58.4 ± 1.61 | 1 | 27.5 ± 0.64 |
2 | 20.91 ± 1.467 | 2 | 39.57 ± 0.592 | 2 | 17.3 ± 1.79 |
3 | 79.6 ± 0.97 | 3 | 53.7 ± 1.03 | 3 | 80.4 ± 1.34 |
Lb. rhamnosus | Lb. paracasei | |
---|---|---|
1 | 3.84 ± 0.67 | 1.82 ± 0.14 |
2 | 3.82 ± 0.73 | 3.81 ± 0.56 |
3 | 2.36 ± 0.76 | 4.55 ± 0.78 |
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Samra, R.M.; Darwish, M.S.; Abou-Zeid, N.A.; Khojah, E.; Imieje, V.O.; Zaki, A.A. Aurones as Antidiabetic Agents and Their Prebiotic Activities. Future Pharmacol. 2023, 3, 625-636. https://doi.org/10.3390/futurepharmacol3030040
Samra RM, Darwish MS, Abou-Zeid NA, Khojah E, Imieje VO, Zaki AA. Aurones as Antidiabetic Agents and Their Prebiotic Activities. Future Pharmacology. 2023; 3(3):625-636. https://doi.org/10.3390/futurepharmacol3030040
Chicago/Turabian StyleSamra, Reham M., Mohamed S. Darwish, Noha A. Abou-Zeid, Ebtihal Khojah, Vincent O. Imieje, and Ahmed A. Zaki. 2023. "Aurones as Antidiabetic Agents and Their Prebiotic Activities" Future Pharmacology 3, no. 3: 625-636. https://doi.org/10.3390/futurepharmacol3030040