Engineering Translation Components Improve Incorporation of Exotic Amino Acids
Abstract
:1. Introduction
2. EF-Tu Engineering to Compensate for Poor Affinity to nPAA-tRNAs
3. Ribosome Engineering to Improve Single Incorporation of Exotic Amino Acids
4. tRNA Engineering to Improve Multiple and Consecutive Incorporations of Exotic Amino Acids
5. Perspectives
Author Contributions
Funding
Conflicts of Interest
Abbreviations
EF-Tu | Elongation factor-thermo unstable |
EF-P | Elongation factor-P |
EF-G | Elongation factor-G |
EF4 | Elongation factor 4 |
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Variant Name | Mutation | Amino Acid | Reference |
---|---|---|---|
E215A | E216A * | bulky a.a. | Doi et al. [12] |
D216A | D217 * | bulky a.a. | Doi et al. [12] |
EF-Sep | H67R, E216N, D217G, F219Y, T229S, N274W | Sep | Park et al. [13] |
EF-Sep21 | H67R, E216V, D217G, F219Y, T229S, N274W | Sep | Lee et al. [14] |
EF-pY | E216V, D217G, F219G | pTyr | Fan et al. [15] |
EF-R1 | H67Y, E216D, D217R, N274R | Sec | Haruna et al. [17] |
EF-Sel1 | H67R, Q98W, E216N, D217K, N274R | Sec | Haruna et al. [17] |
Variant Name | Mutation | Amino Acid | Reference |
---|---|---|---|
A4 | 2447UGGC2450 | D-Phe, D-Met | Dedkova et al. [18] |
B25 | 2457GCUGAU2462 | D-Phe, D-Met | Dedkova et al. [19] |
040329 | 2057AGCGUGA2063, 2502UGGCAG2507 | β-hGly, β-hAla, β, β-dimethyl-β-hGly, β-hPhg, β-(p-Br)hPhg | Dedkova et al. [20] Maini et al. [21] |
P7A7 | 2057AGCGUGA2063, 2502UGACUU2507 | β-(p-Br)hPhg | Czekster et al. [22] |
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Katoh, T.; Suga, H. Engineering Translation Components Improve Incorporation of Exotic Amino Acids. Int. J. Mol. Sci. 2019, 20, 522. https://doi.org/10.3390/ijms20030522
Katoh T, Suga H. Engineering Translation Components Improve Incorporation of Exotic Amino Acids. International Journal of Molecular Sciences. 2019; 20(3):522. https://doi.org/10.3390/ijms20030522
Chicago/Turabian StyleKatoh, Takayuki, and Hiroaki Suga. 2019. "Engineering Translation Components Improve Incorporation of Exotic Amino Acids" International Journal of Molecular Sciences 20, no. 3: 522. https://doi.org/10.3390/ijms20030522