A Novel Dental Sealant Containing Dimethylaminohexadecyl Methacrylate Suppresses the Cariogenic Pathogenicity of Streptococcus mutans Biofilms
Abstract
:1. Introduction
2. Results
2.1. DMAHDM Reduced Biofilm Formation
2.2. DMAHDM Alone and in Combination with NACP Alters Acid and Oxygen Tolerance of S. mutans
2.3. Attenuation of Exopolysaccharide Produced by S. mutans
3. Discussion
4. Materials and Methods
4.1. Development of Dental Resin Sealant Formulations
- Commercial High-viscosity Sealant/Flowable Composite control termed “Commercial Control” (Virtuoso, DenMat, Lompoc, CA, USA).
- Experimental Control Sealant termed “0% DMAHDM + 0% NACP; Experimental Control” (50% PEHB + 0% DMAHDM + 50% Glass + 0% NACP).
- Experimental Sealant termed “5% DMAHDM + 0% NACP” (45% PEHB + 5% DMAHDM + 50% Glass + 0% NACP).
- Experimental Sealant termed “0% DMAHDM + 20% NACP” (50% PEHB + 0% DMAHDM + 30% Glass + 20% NACP).
- Experimental Sealant termed “5% DMAHDM + 20% NACP” (45% PEHB + 5% DMAHDM + 30% Glass + 20% NACP).
4.2. Sample Preparation
4.3. S. mutans Biofilm Formation
4.4. Colony-Forming Unit Counts
4.5. Metabolic Activity
4.6. Acid Stress and Oxygen Stress Tolerance
4.7. Polysaccharide Production
4.8. Acid-Neutralizing Activity
4.9. Lactic Acid Production
4.10. Confocal Laser Scanning Microscopy
4.11. Statistical Analysis
4.12. Disclaimer
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ibrahim, M.S.; Ibrahim, A.S.; Balhaddad, A.A.; Weir, M.D.; Lin, N.J.; Tay, F.R.; Oates, T.W.; Xu, H.H.K.; Melo, M.A.S. A Novel Dental Sealant Containing Dimethylaminohexadecyl Methacrylate Suppresses the Cariogenic Pathogenicity of Streptococcus mutans Biofilms. Int. J. Mol. Sci. 2019, 20, 3491. https://doi.org/10.3390/ijms20143491
Ibrahim MS, Ibrahim AS, Balhaddad AA, Weir MD, Lin NJ, Tay FR, Oates TW, Xu HHK, Melo MAS. A Novel Dental Sealant Containing Dimethylaminohexadecyl Methacrylate Suppresses the Cariogenic Pathogenicity of Streptococcus mutans Biofilms. International Journal of Molecular Sciences. 2019; 20(14):3491. https://doi.org/10.3390/ijms20143491
Chicago/Turabian StyleIbrahim, Maria Salem, Ahmed S. Ibrahim, Abdulrahman A. Balhaddad, Michael D. Weir, Nancy J. Lin, Franklin R. Tay, Thomas W. Oates, Hockin H. K. Xu, and Mary Anne S. Melo. 2019. "A Novel Dental Sealant Containing Dimethylaminohexadecyl Methacrylate Suppresses the Cariogenic Pathogenicity of Streptococcus mutans Biofilms" International Journal of Molecular Sciences 20, no. 14: 3491. https://doi.org/10.3390/ijms20143491