Cytotoxic Effects of Hydroxylated Fullerenes in Three Types of Liver Cells
Abstract
:1. Introduction
2. Results
2.1. Mass Spectrometric Analysis of C60(OH)x
2.2. Cytotoxicity of C60 and C60(OH)x
3. Discussion
4. Experimental Section
4.1. Chemicals
4.2. Sample Preparation
4.3. Cells
4.4. Cell Viability Assay
4.5. Mitochondrial Activity Assay
4.6. Statistical Analysis
5. Conclusions
Acknowledgments
Conflict of Interest
References
- Krätschmer, W.; Lamb, L.D.; Fostiropoulos, K.; Huffman, D.R. C60: A New Form of Carbon. Nature 1990, 347, 354–358. [Google Scholar] [CrossRef]
- Kroto, H.W. The Stability of the Fullerenes Cn, with n = 24, 28, 32, 36, 50, 60 and 70. Nature 1987, 329, 529–531. [Google Scholar] [CrossRef]
- Bordin, V.I.; Trukhacheva, V.A. Thermal Stability of Fullerenes. Tech. Phys. Lett. 2004, 30, 598–599. [Google Scholar] [CrossRef]
- Fortner, J.D.; Lyon, D.Y.; Sayes, C.M.; Boyd, A.M.; Falkner, J.C.; Hotze, E.M.; Alemany, L.B.; Tao, Y.J.; Guo, W.; Ausman, K.D.; Colvin, V.L.; Hughes, J.B. C60 in Water: Nanocrystal Formation and Microbial Response. Environ. Sci. Technol. Lett. 2005, 39, 4307–4316. [Google Scholar] [CrossRef]
- Smalley, R.E.; Kroto, H.W.; Heath, J.R. C60: Buckminsterfullerene. Nature 1985, 318, 162–163. [Google Scholar] [CrossRef]
- Dugan, L.L.; Gabrielsen, J.K.; Yu, S.P.; Lin, T.S.; Choi, D.W. Buckminsterfullerenol Free Radical Scavengers Reduce Excitotoxic and Apoptotic Death of Cultured Cortical Neurons. Neurobiol. Dis. 1996, 3, 129–135. [Google Scholar] [CrossRef] [PubMed]
- Dugan, L.L.; Turetsky, D.M.; Du, C.; Lobner, D.; Wheeler, M.; Almli, C.R.; Shen, C.K.F.; Luh, T.Y.; Choi, D.W.; Lin, T.S. Carboxyfullerenes as Neuroprotective Agents. Proc. Natl. Acad. Sci. USA 1997, 94, 9434–9439. [Google Scholar] [CrossRef] [PubMed]
- Murugan, M.A.; Gangadharan, B.; Mathur, P.P. Antioxidative Effect of Fullerenol on Goat Epididymal Spermatozoa. Asian J. Androl. 2002, 4, 149–152. [Google Scholar] [PubMed]
- Bogdanović, V.; Stankov, K.; Ičević, I.; Žikič, D.; Nikolić, A.; Šolajic, S.; Djordjević, A.; Bogdanović, G. Fullerenol C60(OH)24 Effects on Antioxidative Enzymes Activity in Irradiated Human Erythroleukemia Cell Line. J. Radiat. Res. 2008, 49, 321–327. [Google Scholar] [CrossRef] [PubMed]
- Xiao, L.; Aoshima, H.; Saitoh, Y.; Miwa, N. Highly Hydroxylated Fullerene Localizes at the Cytoskeleton and Inhibits Oxidative Stress in Adipocytes and A Subcutaneous Adipose-Tissue Equivalent. Free Radic. Biol. Med. 2011, 51, 1376–1389. [Google Scholar] [CrossRef] [PubMed]
- Tokuyama, H.; Yamago, S.; Nakamura, E. Photoinduced Biochemical Activity of Fullerene Carboxylic Acid. J. Am. Chem. Soc. 1993, 115, 7918–7919. [Google Scholar] [CrossRef]
- Friedman, S.H.; DeCamp, D.L.; Sijbesma, R.P.; Srdanov, G.; Wudl, F.; Kenyon, G.L. Inhibition of the HIV-1 Protease by Fullerene Derivatives: Model Building Studies and Experimental Verification. J. Am. Chem. Soc. 1993, 115, 6506–6509. [Google Scholar] [CrossRef]
- Yamago, S.; Tokuyama, H.; Nakamura, E.; Kikuchi, K.; Kananishi, S.; Sueki, K.; Nakahara, H.; Enomoto, S.; Ambe, F. In vivo Biological Behavior of a Water-Miscible Fullerene: 14C Labeling, Absorption, Distribution, Excretion and Acute Toxicity. Chem. Biol. 1995, 2, 385–389. [Google Scholar] [CrossRef] [PubMed]
- Bullard-Dillard, R.; Creek, K.E.; Scrivens, W.A.; Tour, J.M. Tissue Sites of Uptake of 14C-Labeled C60. Bioorgan. Chem. 1996, 24, 376–385. [Google Scholar] [CrossRef]
- Nikolić, N.; Vranjes-Ethurić, S.; Janković, D.; Ethokić, D.; Mirković, M.; Bibić, N.; Trajković, V. Preparation and Biodistribution of Radialabeled Fullerene C60 Nanocrystals. Nanotechnology 2009, 20. [Google Scholar] [CrossRef] [PubMed]
- Cagle, D.W.; Kennel, S.J.; Mirzadeh, S.; Alford, J.M.; Wilson, L.J. In vivo Studies of Fullerene-Based Materials Using Endohedral Metallofullerene Radiotracers. Proc. Natl. Acad. Sci. USA 1999, 96, 5182–5187. [Google Scholar] [CrossRef] [PubMed]
- Kubota, R.; Tahara, M.; Shimizu, K.; Sugimoto, N.; Hirose, A.; Nishimura, N. Time-Dependent Variation in the Biodistribution of C60 in Rats Determined by Liquid Chromatography-Tandem Mass Spectrometry. Toxicol. Lett. 2011, 206, 172–177. [Google Scholar] [CrossRef] [PubMed]
- Saathoff, J.G.; Inman, A.O.; Xia, X.R.; Riviere, J.E.; Monteiro-Riviere, N.A. In vitro Toxicity Assessment of Three Hydroxylated Fullerenes in Human Skin Cells. Toxicol. In Vitro. 2011, 25, 2105–2112. [Google Scholar] [CrossRef] [PubMed]
- Wielgus, A.R.; Zhao, B.; Chignell, C.F.; Hu, D.N.; Roberts, J.E. Phototoxicity and Cytotoxicity of Fullerol in Human Retinal Pigment Epithelial Cells. Toxicol. Appl. Pharmacol. 2010, 242, 79–90. [Google Scholar] [CrossRef] [PubMed]
- Yamawaki, H.; Iwai, N. Cytotoxicity of Water-Soluble Fullerene in Vascular Endothelial Cells. Am. J. Physiol. Cell Physiol. 2006, 290, C1495–C1502. [Google Scholar] [CrossRef] [PubMed]
- Nakagawa, Y.; Suzuki, T.; Ishii, H.; Nakae, D.; Ogata, A. Cytotoxic Effects of Hydroxylated Fullerenes on Isolated Rat Hepatocytes via Mitochondrial Dysfunction. Arch. Toxicol. 2011, 85, 1429–1440. [Google Scholar] [CrossRef] [PubMed]
- Ueng, T.H.; Kang, J.J.; Wang, H.W.; Cheng, Y.W.; Chiang, L.Y. Suppression of Microsomal Cytochrome P450-Dependent Monooxygenase and Mitochondrial Oxidative Phosphorylation by Fullerenol, a Polyhydroxylated Fullerene C60. Toxicol. Lett. 1997, 93, 29–37. [Google Scholar] [CrossRef] [PubMed]
- Bogdanović, G.; Kojić, V.; Đorđević, A.; Čanadanović-Brunet, J.; Vojinović-Miloradov, V.; Baltić, V.V. Modulating of Fullerol C60(OH)22 on Doxorubicin-Induced Cytotoxicity. Toxicol. In Vitro. 2004, 18, 629–637. [Google Scholar] [CrossRef] [PubMed]
- Manzetti, S.; Behzadi, H.; Otto, A.; van der Spoel, D. Fullerenes Toxicity and Electronic Properties. Environ. Chem. Lett. 2013, 11, 105–118. [Google Scholar] [CrossRef]
- Johnson-Lyles, D.N.; Peifley, K.; Lockett, S.; Neun, B.W.; Hansen, M.; Clogston, J.; Stern, S.T.; McNeil, S.E. Fullerenol Cytotoxicity in Kidney Cells is Associated with Cytoskeleton Disruption, Autophagic Vacuole Accumulation, and Mitochondrial Dysfunction. Toxicol. Appl. Pharmacol. 2010, 248, 249–258. [Google Scholar] [CrossRef] [PubMed]
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Shimizu, K.; Kubota, R.; Kobayashi, N.; Tahara, M.; Sugimoto, N.; Nishimura, T.; Ikarashi, Y. Cytotoxic Effects of Hydroxylated Fullerenes in Three Types of Liver Cells. Materials 2013, 6, 2713-2722. https://doi.org/10.3390/ma6072713
Shimizu K, Kubota R, Kobayashi N, Tahara M, Sugimoto N, Nishimura T, Ikarashi Y. Cytotoxic Effects of Hydroxylated Fullerenes in Three Types of Liver Cells. Materials. 2013; 6(7):2713-2722. https://doi.org/10.3390/ma6072713
Chicago/Turabian StyleShimizu, Kumiko, Reiji Kubota, Norihiro Kobayashi, Maiko Tahara, Naoki Sugimoto, Tetsuji Nishimura, and Yoshiaki Ikarashi. 2013. "Cytotoxic Effects of Hydroxylated Fullerenes in Three Types of Liver Cells" Materials 6, no. 7: 2713-2722. https://doi.org/10.3390/ma6072713