Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions
Abstract
:1. Introduction
2. Methods
2.1. Materials
2.2. Preparation of Shellac Microcapsules of Lactoferrin
2.3. Study of Microcapsule Morphology and Size Distribution
2.4. Lf microcapsule In Vitro Release
2.5. Statistical Analysis
3. Results and Discussion
3.1. Encapsulation of Lf in Shellac by PGSS
3.2. Fourier Transform Infra-Red Spectroscopy (FT-IR) Analysis
3.3. In Vitro Release
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Experimental Conditions | Avg. Particle Size [µm] | Particle Size Distribution (PSD) | EE [%] | ||||||
---|---|---|---|---|---|---|---|---|---|
P [MPa] | T [°C] | Lactoferrin [g] | Shellac EtOH Ratio | d0.1 | d0.5 | d0.9 | Span | ||
8 | 40 | 0.2 | 1:10 | 143.0 ± 4 b | 61.8 | 144.3 | 338.5 | 1.91 | 51 ± 5 a |
9 | 40 | 0.2 | 1:10 | 132.6 ± 10 b | 39.8 | 153.0 | 592.0 | 3.61 | |
10 | 40 | 0.2 | 1:10 | 75.4 ± 7 a | 36.8 | 77.2 | 161.3 | 1.61 | 71 ± 2 b |
10 | 50 | 0.2 | 1:10 | Agglomerated | - | - | - | - | - |
10 | 30 | 0.2 | 1:10 | 59.8 ± 2 a | 5.09 | 59.9 | 559.8 | 9.92 | - |
10 | 20 | 0.2 | 1:10 | 71.4 ± 3 a | 12.17 | 77.56 | 423.3 | 5.30 | - |
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Ono, K.; Sakai, H.; Tokunaga, S.; Sharmin, T.; Aida, T.M.; Mishima, K. Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions. Processes 2021, 9, 73. https://doi.org/10.3390/pr9010073
Ono K, Sakai H, Tokunaga S, Sharmin T, Aida TM, Mishima K. Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions. Processes. 2021; 9(1):73. https://doi.org/10.3390/pr9010073
Chicago/Turabian StyleOno, Kento, Hiroki Sakai, Shinichi Tokunaga, Tanjina Sharmin, Taku Michael Aida, and Kenji Mishima. 2021. "Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions" Processes 9, no. 1: 73. https://doi.org/10.3390/pr9010073