Antibiotic Detection in Animal-Derived Agricultural Products

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Farm Animal Production".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 586

Special Issue Editors


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Guest Editor
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Interests: food safety; food quality; sample preparation; rapid detection technology; animal-derived food; antibiotic residues
Special Issues, Collections and Topics in MDPI journals
College of Food Science and Engineering, Yangzhou University, Yangzhou 225009, China
Interests: food safety; sample preparation; rapid detection technology; animal-derived food; antibiotic residues
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Antibiotic residues in animal-derived agricultural products (e.g., meat, eggs, milk, and honey) pose significant risks to food safety and public health by contributing to antibiotic resistance and potential allergic reactions, necessitating effective detection methods. Current approaches include highly sensitive instrumental techniques such as liquid chromatography–tandem mass spectrometry (LC-MS/MS), gas chromatography–mass spectrometry (GC-MS), and high-performance liquid chromatography (HPLC), typically combined with sample preparation methods like solid-phase extraction (SPE), QuEChERS, or accelerated solvent extraction (ASE), as well as rapid screening methods for on-site and high-throughput testing, including enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) for resistance gene detection, and biosensors (e.g., optical, electrochemical). Emerging technologies like nanomaterials-based sensors and artificial intelligence-assisted analysis show promise for improving sensitivity and reducing detection time, highlighting the need for continuous innovation to ensure regulatory compliance and consumer safety. We also encourage the submission of review articles covering diverse detection technologies for antibiotic residues in animal-derived agricultural products.

Prof. Dr. Genxi Zhang
Dr. Bo Wang
Guest Editors

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Keywords

  • antibiotics
  • animal-derived agricultural products
  • detection method
  • sample pretreatment method
  • rapid detection technology
  • nondestructive testing technology

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Published Papers (1 paper)

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Research

18 pages, 5659 KB  
Article
Efficient Determination of β-Agonists in Environmental Water and Animal-Derived Matrices by NH2-UiO-66 Based d-SPE Coupled with UPLC-MS/MS: Performance, Mechanism and Application
by Chujun Liu, Yuliang Xu, Sihan Wang, Boyan Sun, Zimo Liu, Qian Ran, Jiankang Ren, Zhiyue Feng, Jie Xie and Haiyang Jiang
Agriculture 2026, 16(5), 519; https://doi.org/10.3390/agriculture16050519 - 26 Feb 2026
Viewed by 381
Abstract
β-agonists are prohibited antibiotics that have raised concerns due to their illegal use in the livestock industry, posing potential toxicity risks to human health. For ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis of β-agonists, effective sample pretreatment is a crucial and [...] Read more.
β-agonists are prohibited antibiotics that have raised concerns due to their illegal use in the livestock industry, posing potential toxicity risks to human health. For ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis of β-agonists, effective sample pretreatment is a crucial and challenging process that dictates the overall reliability and sensitivity of the method. Thus, this study developed a reliable method utilizing dispersive solid-phase extraction (d-SPE) with NH2-UiO-66 as a superior adsorbent, coupled with UPLC-MS/MS, to extract and quantify β-agonists in environmental water, swine urine, and milk. The synthesized NH2-UiO-66 exhibited outstanding adsorption capacities (146.06–358.00 mg/g) towards the target analytes. The optimized method demonstrated excellent performance: low matrix effects (−13.10–15.30%), wide linearity (0.1–50 μg/L), low limits of detection (0.04–0.09 μg/L), and satisfactory recoveries (81.48–106.67%) with good precision (intra-day RSDs 1.51–6.24%; inter-day RSDs 2.06–10.96%). Adsorption mechanism studies revealed that the extraction process, which followed the Langmuir isotherm and pseudo-second-order kinetic models, was driven primarily by electrostatic interactions, π-π stacking, and hydrogen bonding. Moreover, the material could be reused up to 10 times, with satisfactory recoveries of 81.30% to 116.10%. The proposed NH2-UiO-66-d-SPE-UPLC-MS/MS protocol is generic and provides a robust and practical solution for monitoring trace β-agonists in animal-derived foods and environmental samples, ensuring food safety and environmental health. Full article
(This article belongs to the Special Issue Antibiotic Detection in Animal-Derived Agricultural Products)
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