Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.9
3.3
Journals
Agriculture
Special Issues
Targeted Rice Improvement through Genome Editing
share announcement

Targeted Rice Improvement through Genome Editing

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Crop Genetics, Genomics and Breeding".

Deadline for manuscript submissions: closed (15 February 2023) | Viewed by 2617

Special Issue Editors

Dr. Gaoneng Shao

E-Mail Website
Guest Editor
State Key Laboratory of Rice Biology and China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
Interests: rice; rice quality and safety; genetics; QTL-mapping; genome wide association studies; gene editing technology; molecular design breeding
Dr. Shakeel Ahmad

E-Mail Website
Guest Editor
National Center for Genome Editing for Crop Improvement and Human Health, Center for Advanced Studies in Agriculture and Food Security, University of Agriculture Faisalabad, Punjab, Pakistan
Interests: rice genetics and genomics; gene editing; CRISPR/Cas9; photosynthesis; chlorophyll; plant physiology; abiotic stresses

Special Issue Information

Dear Colleagues,

The world’s population will reach about 9.6 billion by 2050, and the demand for staple crops is also expected to increase by 60%. Rice is one of the most important food crop and is consumed by almost half of the world’s population, meaning that more rice needs to be produced to meet demand. Recently, CRISPR–Cas-based gene editing has been developed as a powerful tool and has been ued to improve several rice characteristics, including yield, quality, biotic stress resistance, and abiotic stress tolerance. Gene editing strategies have also rapidly revolutionized in the past ten years due to the targeted knockout of a single or multiple genes by the CRISPR–Cas system, resulting in precise gene editing with base editing or prime editing technology. Interestingly, numerous plant varieties created by gene editing technologies are available for commercial cultivation in some developed countries, implying genome editing systems could be implemented for rice varieties.

This Special Issue, “Targeted Rice Improvement through Genome Editing”, encourages the submission of original and/or review articles related to the application of genome editing techniques in rice. Possible targeted traits include grain yield, grain quality and nutrition, and biotic and abiotic stress tolerance.

Dr. Gaoneng Shao
Dr. Shakeel Ahmad
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agriculture is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • rice
  • CRISPR/Cas9
  • gene editing
  • crop improvement
  • food security

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

13 pages, 2951 KiB  
Article
Base Editing of EUI1 Improves the Elongation of the Uppermost Internode in Two-Line Male Sterile Rice Lines
by Yakun Wang, Shengjia Tang, Naihui Guo, Ruihu An, Zongliang Ren, Shikai Hu, Xiangjin Wei, Guiai Jiao, Lihong Xie, Ling Wang, Ying Chen, Fengli Zhao, Peisong Hu, Zhonghua Sheng and Shaoqing Tang
Agriculture 2023, 13(3), 693; https://doi.org/10.3390/agriculture13030693 - 16 Mar 2023
Viewed by 1778
Abstract
The use of male sterile lines (MSLs) of rice is essential for heterosis utilization. However, MSLs have a common defect in the elongation of the uppermost internode, eventually leading to incomplete panicle exsertion, blocking pollination, and reducing the hybrid rice seed yield. Previously, [...] Read more.
The use of male sterile lines (MSLs) of rice is essential for heterosis utilization. However, MSLs have a common defect in the elongation of the uppermost internode, eventually leading to incomplete panicle exsertion, blocking pollination, and reducing the hybrid rice seed yield. Previously, the elongated uppermost internode 1 (EUI1) was identified as an active gibberellin-deactivating enzyme that plays a key role in panicle exsertion from the flag leaf sheath in rice (Oryza sativa L.). We used an adenine base editor to edit EUI1 and obtained two types of homozygous transgenic plants (eui1-1 and eui1-2). The transcription and translation levels of EUI1 in the two mutants were significantly lower than in the wild type, as was the oxidation activity of EUI1 to active gibberellins (GAs), which also decreased. The contents of the plant hormones GA1, GA3, and GA4 in eui1-1 (1.64, 1.55, and 0.92 ng/g) and eui1-2 (0.85, 0.64, and 0.65 ng/g) panicles were significantly higher than the wild type (0.70, 0.57, and 0.42 ng/g). The uppermost internode lengths of the mutant were 26.5 and 23.6 cm, which were significantly longer than that of the wild type (18.0 cm), and the cell lengths of the mutant were 161.10 and 157.19 μm, which were longer than that of the wild type (89.28 μm). Our results indicate that the adenine base editing system could increase the content of endogenous bioactive GAs in young panicles by fine-tuning EUI1 activity, reduce the defect of panicle enclosure in MSLs and increase the yield of hybrid rice seed production. Full article
(This article belongs to the Special Issue Targeted Rice Improvement through Genome Editing)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop