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Agriculture
Special Issues
Pathology and Veterinary Diagnostics of Farming Animals
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Pathology and Veterinary Diagnostics of Farming Animals

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Farm Animal Production".

Deadline for manuscript submissions: closed (25 February 2024) | Viewed by 3368

Special Issue Editor

Prof. Dr. Tianle Xu

E-Mail Website
Guest Editor
Agricultural Science and Technology Development Research Institute, Yangzhou University, Yangzhou 225009, China
Interests: bovine mastitis; host–pathogen interactions; lipid metabolic disorders; development of antimicrobial

Special Issue Information

Dear Colleagues,

In recent years, the prevalence of pathogenic bacteria has constrained the economic development of the livestock industry, but the treatment of pathogenic bacteria is still dominated by antibiotics, which has led to the emergence of drug-resistant strains. The extensive utilization of antibiotics has accelerated the spread of drug-resistant strains, so the severity of diseases caused by pathogenic bacteria is assessed according to the origin, transmission route, and clinical manifestations of the pathogenic bacteria. However, some pathogenic bacteria can cause zoonotic diseases, and the early detection of pathogenic infections can help to cut off transmission routes and may prevent epidemics of certain pathogens from spreading between humans and domestic animals. The purpose of this Special Issue is to explore the impact of pathogenic bacteria on livestock with respect to the study of pathology and the development of rapid diagnosis techniques. We welcome the submission of original research articles or reviews addressing pathogenic bacteria in farming animals. We will also welcome investigations on the clinical signs of and pathologic changes caused by pathogenic bacteria, therapeutic measures, and on drug-resistant strains. Potential topics include, but are not limited to, mechanisms of resistance, pathogenesis, and potential preventive measures that will help establish disease prevention and control.

Prof. Dr. Tianle Xu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agriculture is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bacteria
  • drug-resistant strains
  • pathological
  • host-immune responses
  • metabolic disorders
  • establishment of novel diagnostic approaches
  • farming animals

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Published Papers (2 papers)

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Research

13 pages, 3770 KiB  
Article
Involvement of Pyocyanin in Promoting LPS-Induced Apoptosis, Inflammation, and Oxidative Stress in Bovine Mammary Epithelium Cells
by Hao Zhu, Wendi Cao, Yicai Huang, Niel A. Karrow and Zhangping Yang
Agriculture 2023, 13(12), 2192; https://doi.org/10.3390/agriculture13122192 - 23 Nov 2023
Viewed by 1262
Abstract
Pyocyanin (PCN) is an extracellular toxin secreted by Pseudomonas aeruginosa (PA), which has redox capacity and disrupts the redox balance of host cells, affecting cell function and leading to cell death. The aim of this experiment was to compare the degree of apoptosis, [...] Read more.
Pyocyanin (PCN) is an extracellular toxin secreted by Pseudomonas aeruginosa (PA), which has redox capacity and disrupts the redox balance of host cells, affecting cell function and leading to cell death. The aim of this experiment was to compare the degree of apoptosis, inflammation, and oxidative stress of bovine mammary epithelium cells (bMECs) induced by lipopolysaccharide (LPS) and pyocyanin (PCN) and to examine whether PCN can promote the apoptosis, inflammation, and oxidative stress of bMECs induced by LPS. In this study, 1 µg/mL LPS and 1 µg/mL PCN were finally selected for subsequent experiments through dose-dependent experiments. In this study, cells were not given any treatment and were used as the control group (NC). The cells were treated with PCN or LPS individually for 6 h as the PCN group (PCN) or the LPS group (LPS), and the combination of LPS and PCN challenge for 6 h as the LPS + PCN (LPS + PCN) group. Compared with the control and LPS groups, PCN resulted in a significantly upregulated expression of genes related to pro-inflammatory (IL-6, TNF-α, MyD88), apoptotic (Bax, Caspase3, Caspase9), as well as protein expression of components in the TLR4/NF-κB signaling pathway (TLR4, p-p65, p65), and p53 signaling pathway (p-p53, p53, Caspase9) (p < 0.05). Moreover, the expression of genes and proteins was significantly upregulated after PCN treatment combined with LPS compared to either LPS or PCN challenge alone (p < 0.05). The stimulation of PCN combined with LPS significantly increased reactive oxygen species (ROS) and malondialdehyde (MDA) production in bovine mammary epithelial cells (bMECs), as well as decreased glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC). Moreover, cells in the LPS + PCN group aggravated oxidative stress and antioxidant inhibition in cells. In addition, the expression of the corresponding genes and proteins related to the Nrf2 pathway (Nrf2, HO-1) was significantly down-regulated in the PCN group as compared to the control group (p < 0.05). Altogether, PCN stimulation exacerbates inflammatory reactions, apoptosis, and oxidative stress reactions, as well as when combined with LPS challenge in bMECs. Therefore, this study indicates that PCN manifests a role in promoting apoptosis, inflammation, and oxidative stress and interacting with LPS to enhance more serious biological stress responses. Full article
(This article belongs to the Special Issue Pathology and Veterinary Diagnostics of Farming Animals)
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12 pages, 1416 KiB  
Article
Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of Klebsiella pneumoniae in Jiangsu, China
by Wendi Cao, Yi Xu, Yicai Huang and Tianle Xu
Agriculture 2023, 13(10), 1984; https://doi.org/10.3390/agriculture13101984 - 12 Oct 2023
Cited by 2 | Viewed by 1725
Abstract
According to recent investigations, the proportion of mastitis caused by environmental pathogens, such as K. pneumoniae, has increased. In this research, the epidemiology of pathogens in milk samples collected from four farms in the Jiangsu Province was carried out. The results show [...] Read more.
According to recent investigations, the proportion of mastitis caused by environmental pathogens, such as K. pneumoniae, has increased. In this research, the epidemiology of pathogens in milk samples collected from four farms in the Jiangsu Province was carried out. The results show that 16 pathogens were detected in 186 positive milk samples. It was found that K. pneumoniae had the lowest sensitivity to penicillin (0%) and amoxicillin (4%) compared to its sensitivity to gentamicin (92%) and piperacillin (89%). A total of eight ESBL-producing strains were detected. Crystal violet staining showed that 46 of the 68 isolates of K. pneumoniae had strong biofilm-forming ability, which was related to the tetracycline resistance phenotype (p < 0.05). The detection rate of the ESBL-resistant gene (blaSHV) reached 100%. The results show that resistance genes blaVIM, blaOXA-10, and blaTEM were correlated with drug-resistance phenotypes to varying degrees. The present study indicates the prevalence of bovine mastitis-derived pathogens in part of Jiangsu Province and reveals the distribution of β-lactam resistance genes and the strong biofilm-forming ability of K. pneumoniae and its relationship with tetracycline resistance. This study provided theoretical support and guidance for rational drug use and disease prevention and control on farms. Full article
(This article belongs to the Special Issue Pathology and Veterinary Diagnostics of Farming Animals)
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