Qualitative and Quantitative Plant Screening Measurements for Yield and Quality Enhancement

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: 30 November 2024 | Viewed by 1482

Special Issue Editors


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Guest Editor
Department of Industrial Plants Breeding and Genetics, Agricultural Institute Osijek, 31000 Osijek, Croatia
Interests: industrial plants breeding; crop production; crop science; climate change; abiotic stress; photosynthesis; agronomy

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Guest Editor
Department of Maize Breeding and Genetics, Agricultural Institute Osijek, 31000 Osijek, Croatia
Interests: genetics and physiology of multiple-stress tolerance; maize breeding; contents of carotenoids and tools in maize grain; genotype by environment interaction in crops
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Special Issue Information

Dear Colleagues,

In the context of changing climatic conditions, plant breeders face the challenge of creating varieties/hybrids that are adaptable, i.e., tolerant to rapidly changing and extreme weather events, as well as biotic stressors during plant vegetation. The final goal is to develop genotypes that can achieve acceptable yields in variable environmental conditions. In a world where human hunger is on the rise, it is crucial to prioritise not only the quantity of plant production used for food or feed, but also the quality of plant products.

Plant breeding programmes necessitate easy and quick tools and methods for selecting stress-tolerant genotypes. One of these tools is non-invasive chlorophyll fluorescence measurement. It is commonly applied to enhance comprehension of the mechanism of photosynthesis and studying the effects of adverse biotic and abiotic conditions on plant development. Although there are numerous studies linking chlorophyll fluorescence measurements and photosynthesis with plant physiology traits in favourable or unfavourable environments, only some of these studies have examined the relationship of photosynthetic traits with agronomic traits, plant product quality traits or yield as the ultimate goal of all crop production. Chlorophyll fluorescence measurement can be beneficial in screening for stress-tolerant genotypes, but this tolerance needs to be quantified in terms of plant yield quantity and quality to ensure the economic effectiveness of breeding programmes. Although chlorophyll fluorescence measurement is the most widely used qualitative method of detecting stress in plants, its limitation is the need for the dark adaptation of plant tissue before measurement. Other qualitative measures, such as thermography and VIS/NIR reflectance, which do not require dark adaptation, can provide continuous monitoring of extensive plant fields, increasing the throughput of data collection. These novel, high-throughput germplasm phenotyping methods are being increasingly investigated, but still need validation for more plant species.

For all the reasons mentioned above, this Special Issue aims to determine the association of chlorophyll fluorescence parameters and physiology traits with plant product quality and yield. It also aims to further investigate the use of infrared thermal imaging and VIS/NIR reflectance to increase germplasm screening and data collection efficiency, thus accelerating the selection of adaptable plants with desirable traits. This Special Issue welcomes all research papers exploring the correlation between chlorophyll fluorescence parameters, physiological traits, yield and plant product quality, and those validating infrared thermal imaging and VIS/NIR reflectance for assessing plant conditions in favourable and unfavourable environments.

Dr. Antonela Markulj Kulundžić
Dr. Domagoj Šimić
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chlorophyll a fluorescence
  • photosynthesis
  • VIS/NIR reflectance
  • thermal imaging
  • spectral reflectance indices
  • phytochemical reflectance index
  • plant physiology
  • breeding
  • crop production
  • agronomic traits
  • yield
  • yield components
  • plant quality genotype
  • unfavourable growth conditions
  • adaptation
  • tolerance
  • resistance

Published Papers (2 papers)

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Research

25 pages, 6205 KiB  
Article
Effects of Drought at Anthesis on Flag Leaf Physiology and Gene Expression in Diverse Wheat (Triticum aestivum L.) Genotypes
by Jurica Duvnjak, Hrvoje Sarcevic, Rosemary Vukovic and Valentina Spanic
Agronomy 2024, 14(7), 1522; https://doi.org/10.3390/agronomy14071522 - 12 Jul 2024
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Abstract
The current study aimed to quantify the effects of two drought intensities achieved by deprivation of watering for 45 and 65% of the volumetric soil moisture content (VSMC) for 14 days after wheat anthesis to identify physio-biochemical and molecular changes associated with drought [...] Read more.
The current study aimed to quantify the effects of two drought intensities achieved by deprivation of watering for 45 and 65% of the volumetric soil moisture content (VSMC) for 14 days after wheat anthesis to identify physio-biochemical and molecular changes associated with drought tolerance in six genotypes with different drought tolerance. Drought at 65% of VSMC induced a significant decrease in the chlorophyll a content in the drought-sensitive genotype, which indicated a strong loss of photosynthetic reaction centres. Further, in the drought-tolerant genotype, the content of carotenoids, which could play a vital role in resisting water shortage stress, tended to increase. The increased production of malondialdehyde showed that the antioxidant system in the drought-sensitive genotypes was not properly activated. A significant decrease in catalase (CAT; EC 1.11.1.6) was observed at a 45% reduction in VSMC, compared to the control, in the drought-sensitive genotype, and at a reduction in VSMC of 65%, in all medium sensitive genotypes. Further, the drought-tolerant and -medium tolerant genotypes responded to drought with a decline in total glutathione concentrations with the intention to reinforce their defence system. Thereby, dehydroascorbate reductase (DHAR; EC 1.8.5.1), monodehydroascorbate reductase (MDHAR; EC 1.6.5.4), and glutathione reductase (GR; EC 1.6.4.2) were critical enzymes involved in the ascorbate–glutathione cycle together with CAT, showing their main role in the detoxification of ROS produced with the reduction in VSMC by 65%. The results of gene expression analysis showed that severe drought increased the levels of the DHN5 and WZY2 genes (that were significantly positively correlated) in the drought-tolerant genotype, whose grain weight, area, and length did not change in maturity. Also, it was seen that DHN5 expression showed a significant positive correlation with grain length and proline content at a 45% reduction in VSMC. The identification of different mechanisms under drought can contribute to the selection of drought-tolerant genotypes. Full article
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21 pages, 5735 KiB  
Article
Drought-Stressed Apple Tree Grafted onto Different Rootstocks in a Coastal Sandy Soil: Link between Fast Chlorophyll a Fluorescence and Production Yield
by Andrea Colpo, Sara Demaria, Marzio Zaccarini, Alessandro Forlani, Antonia Senatore, Elena Marrocchino, Angela Martina and Lorenzo Ferroni
Agronomy 2024, 14(6), 1304; https://doi.org/10.3390/agronomy14061304 - 16 Jun 2024
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Abstract
Domesticated apple is a drought-sensitive species that spread from continental to Mediterranean temperate regions, where it can particularly experience prolonged water stress. One strategy to improve drought resistance in apple is engrafting on selected rootstocks. This study explores the potential of fast chlorophyll [...] Read more.
Domesticated apple is a drought-sensitive species that spread from continental to Mediterranean temperate regions, where it can particularly experience prolonged water stress. One strategy to improve drought resistance in apple is engrafting on selected rootstocks. This study explores the potential of fast chlorophyll a fluorescence for the comparison of rootstock sensitivity to drought, looking for significant correlations with fruit productivity. The experiment was conducted in a field located in the coastal Po River Plain, Northern Italy, characterized by a loamy sandy soil, particularly prone to drought (86% sand). Mature plants of apple cv. Superchief® Sandidge engrafted on three different rootstocks (CIVP21pbr, MM106, M26) were monitored throughout the summer of 2021 and compared between irrigated and non-irrigated parcels, and at the end of the season, fruit production was evaluated. Despite soil water tension only reaching −13 kPa, the non-irrigated plants experienced a small but consistent loss of Photosystem II (PSII) activity and a lesser capacity of light energy conservation in the photosynthetic electron transport chain. The fruit weight correlated with PSII photochemical indexes recorded during early drought, particularly FV/FM and PIABS; a correlation emerged between fruit number per plant and median values of electron transport parameters, including PITOT. Although all rootstocks underwent a 40% loss of productivity, the fluorescence parameters revealed a graded susceptibility to drought, M26 > CIVP21pbr > MM106, which matched well with the plant vigour. The least drought-sensitive MM106 produced less numerous but heavier fruits than the other two rootstocks. Full article
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