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5.2
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Journals
Animals
Special Issues
Assisted Reproductive Technologies in Production Animals
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Assisted Reproductive Technologies in Production Animals

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 691

Special Issue Editors

Dr. Andrés Gambini

E-Mail Website
Guest Editor
School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD 4343, Australia
Interests: sperm; oocyte; cattle; activation; embryo; cryopreservation; equine; donkey; in vitro fertilization
Special Issues, Collections and Topics in MDPI journals
Dr. Rafael Fernández-Martín

E-Mail Website
Guest Editor
School of Agriculture, Universidad de Buenos Aires, Av San Martin 4453, Buenos Aires 1416, Argentina
Interests: embryos; in vitro fertilization; gene-edition; pig; cattle; cloning

Special Issue Information

Dear Colleagues,

Advancements in assisted reproductive technologies (ARTs) are transforming modern livestock breeding by bridging traditional animal husbandry practices with cutting-edge scientific innovations. Techniques such as in vitro fertilization (IVF), embryo transfer, intracytoplasmic sperm injection (ICSI), and genomic selection enable precise genetic improvements, enhancing productivity, disease resistance, and animal welfare. Moreover, these tools play an increasingly important role in conserving rare and endangered livestock breeds, thereby preserving genetic diversity crucial for sustainable agriculture. By integrating ART with emerging technologies, such as artificial intelligence-driven data analytics, CRISPR-based gene editing, and stem cell-based bioengineering, producers could more effectively meet the growing global demand for high-quality animal products while minimizing environmental impact. This Special Issue invites original research articles, reviews, and case studies focusing on the practical applications, current challenges, and future directions of ART in production animals. Through this collaborative platform, we aim to advance knowledge, foster industry adoption of emerging new practices and contribute to optimizing animal productivity and well-being.

Dr. Andrés Gambini
Dr. Rafael Fernández-Martín
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • assisted reproductive technologies
  • livestock breeding
  • in vitro fertilization
  • embryo transfer
  • intracytoplasmic sperm injection
  • genetic improvement
  • sustainable agriculture
  • animal welfare
  • cloning
  • in vitro breeding
  • stem cells

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

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Research

11 pages, 985 KB  
Article
Cryoinjuries in Cryopreserved Semen of Ichthyoelephas longirostris with Ethylene Glycol
by Jaider Alonso Martínez-Suarez, José Alonso Espinosa-Araujo and Víctor Atencio-García
Animals 2025, 15(16), 2338; https://doi.org/10.3390/ani15162338 - 10 Aug 2025
Viewed by 353
Abstract
Ichthyoelephas longirostris, commonly known as pataló, is an endemic species from Colombia, categorized as endangered due to habitat degradation and overfishing. In this context, semen cryopreservation emerges as an effective strategy for its conservation. This study evaluated the damage caused by semen [...] Read more.
Ichthyoelephas longirostris, commonly known as pataló, is an endemic species from Colombia, categorized as endangered due to habitat degradation and overfishing. In this context, semen cryopreservation emerges as an effective strategy for its conservation. This study evaluated the damage caused by semen cryopreservation using a cryosolution composed of ethylene glycol (EG) at three inclusion levels (6%, 8%, and 10% v/v), skimmed milk powder (3% w/v), and glucose (6% w/v), all prepared in distilled water. A randomized experimental design was employed, with three treatments and a control (fresh semen), using pooled semen from different males (n = 12). Each sample was analyzed in triplicate. Sperm parameters were evaluated through computer-assisted analysis (CASA), and damage to the plasma membrane, mitochondria, and DNA was assessed via flow cytometry. Fresh semen showed low levels of membrane damage (9.6 ± 6.9%), mitochondrial damage (29.1 ± 16.8%), and DNA fragmentation (0.24 ± 0.13%). In thawed semen, the highest membrane damage was observed with 10% EG (79.3 ± 13.0%), while the highest DNA fragmentation occurred with 6% EG (22.5 ± 21.9%). The cryosolution composed of 8% EG showed the best results, with lower levels of damage in the evaluated structures. These findings suggest that the combination of 8% EG, 3% skimmed milk powder, and 6% glucose is an effective formulation to reduce cellular damage during I. longirostris semen cryopreservation, representing a useful tool for conservation programs of this species. Full article
(This article belongs to the Special Issue Assisted Reproductive Technologies in Production Animals)
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