Recent Advances of Swine Viral Disease Research in China: Epidemiology, Virus-Host Interactions and Control: Second Edition

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: 31 October 2025 | Viewed by 1668

Special Issue Editors


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Guest Editor
Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences (CAAS), Harbin 150069, China
Interests: virus–host interactions involved in the regulation of African swine fever virus; classical swine fever virus replication
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences (CAAS), Harbin 150069, China
Interests: classical swine fever; African swine fever; pseudorabies; vaccines; innate and adaptive immunity; virus-host interactions; pathogenesis; diagnostic assays
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

China has the largest pig industry and is the biggest consumer of pork products around the world. In recent decades, endemic and emerging swine viral diseases, such as African swine fever virus (ASFV), classical swine fever (CSFV), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), porcine circovirus 4 (PCV4), swine acute diarrhea syndrome coronavirus (SADS-CoV), and foot-and-mouth disease virus (FMDV), have resulted in huge economic losses in China. To date, Chinese scientists have achieved great progress in preventing and controlling swine viral diseases. This Special Issue on “Recent Advances of Swine Viral Disease Research in China: Epidemiology, Virus–Host Interactions and Control: Second Edition” aims to share knowledge and experience in the aforementioned research fields in China.

In this Special Issue, we will cover the following topics of interest to improve our understanding of the biological characteristics of these viruses: epidemiology, viral genetic diversity and evolution, new serological/molecular diagnostics, virus structure, virus–cell interactions, vaccines and antivirals, and control and prevention measures. We welcome original research articles and reviews including—but not limited to—all the abovementioned areas.

The only limitation is that the main part of the study has to have been carried out in China or by Chinese researchers.

Prof. Dr. Su Li
Prof. Dr. Hua-Ji Qiu
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • epidemiology
  • diagnostic
  • virus–host interactions
  • viral replication, control, and prevention
  • vaccines
  • antivirals
  • emergence and evolution
  • viral immunology
  • viral structure

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Related Special Issue

Published Papers (2 papers)

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Research

16 pages, 1689 KiB  
Article
A Quadruplex RT-qPCR for the Detection of Porcine Sapelovirus, Porcine Kobuvirus, Porcine Teschovirus, and Porcine Enterovirus G
by Biao Li, Kaichuang Shi, Yuwen Shi, Shuping Feng, Yanwen Yin, Wenjun Lu, Feng Long, Zuzhang Wei and Yingyi Wei
Animals 2025, 15(7), 1008; https://doi.org/10.3390/ani15071008 - 31 Mar 2025
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Abstract
Porcine sapelovirus (PSV), porcine kobuvirus (PKV), porcine teschovirus (PTV), and porcine enterovirus G (EV-G) are all important viruses in the swine industry. These viruses play important roles in the establishment of similar clinical signs of diseases in pigs, including diarrhea, encephalitis, and reproductive [...] Read more.
Porcine sapelovirus (PSV), porcine kobuvirus (PKV), porcine teschovirus (PTV), and porcine enterovirus G (EV-G) are all important viruses in the swine industry. These viruses play important roles in the establishment of similar clinical signs of diseases in pigs, including diarrhea, encephalitis, and reproductive and respiratory disorders. The early accurate detection of these viruses is crucial for dealing with these diseases. In order for the differential detection of these four viruses, specific primers and TaqMan probes were designed for the conserved regions in the 5′ untranslated region (UTR) of these four viruses, and one-step quadruplex reverse-transcription real-time quantitative PCR (RT-qPCR) for the detection of PSV, PKV, PTV, and EV-G was developed. The results showed that this assay had the advantages of high sensitivity, strong specificity, excellent repeatability, and simple operation. Probit regression analysis showed that the assay obtained low limits of detection (LODs) for PSV, PKV, PTV, and EV-G, with 146.02, 143.83, 141.92, and 139.79 copies/reaction, respectively. The assay showed a strong specificity of detecting only PSV, PKV, PTV, and EV-G, and had no cross-reactivity with other control viruses. The assay exhibited excellent repeatability of the intra-assay coefficient of variation (CV) of 0.28–1.58% and the inter-assay CV of 0.20–1.40%. Finally, the developed quadruplex RT-qPCR was used to detect 1823 fecal samples collected in Guangxi Province, China between January 2024 and December 2024. The results indicated that the positivity rates of PSV, PKV, PTV, and EV-G were 15.25% (278/1823), 21.72% (396/1823), 18.82% (343/1823), and 27.10% (494/1823), respectively, and there existed phenomena of mixed infections. Compared with the reference RT-qPCR/RT-PCR established for these four viruses, the coincidence rates for the detection results of PSV, PKV, PTV, and EV-G reached 99.51%, 99.40%, 99.51%, and 99.01%, respectively. In conclusions, the developed quadruplex RT-qPCR could simultaneously detect PSV, PKV, PTV, and EV-G, and provided an efficient and convenient detection method to monitor the epidemic status and variation of these viruses. Full article
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12 pages, 878 KiB  
Article
Frequency and Genetic Analysis of Porcine Circovirus Type 2, Which Circulated between 2014 and 2021 in Jiangsu, China
by Qi Xiao, Meng Qu, Jianping Xie, Cigen Zhu, Yuping Shan, Aihua Mao, Wenxian Qian, Jiaping Zhu, Jiahui Guo, Dong Lang, Jiaqiang Niu, Libin Wen and Kongwang He
Animals 2024, 14(19), 2882; https://doi.org/10.3390/ani14192882 - 7 Oct 2024
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Abstract
Porcine circovirus-associated diseases, caused by porcine circovirus type 2 (PCV2), are widespread and result in significant economic losses to the global swine industry. PCV2 can currently be divided into nine genotypes (PCV2a to PCV2i), with the currently dominant one being the PCV2d genotype. [...] Read more.
Porcine circovirus-associated diseases, caused by porcine circovirus type 2 (PCV2), are widespread and result in significant economic losses to the global swine industry. PCV2 can currently be divided into nine genotypes (PCV2a to PCV2i), with the currently dominant one being the PCV2d genotype. In this study, 2675 samples from 804 pig farms in 13 cities in Jiangsu Province, China, were collected between 2014 and 2021 and subjected to polymerase chain reaction analysis to investigate the frequency and genetic diversity of PCV2. The results showed that 41.42% (1108/2675) of samples tested positive for PCV2. The researchers further analyzed the genetic characteristics of 251 PCV2 strains and found that they belonged to the following four genotypes: PCV2a, PCV2b, PCV2d, and PCV2i. The dominant genotype was PCV2d, with a frequency of 49.80% (125/251). The detection rate of PCV2b was significantly higher than those of PCV2a and PCV2i, at 35.46% (89/251), 7.57% (19/251), and 7.17% (18/251), respectively. The percentage of different genotypes of PCV2 varied irregularly over time. We have further revealed the fingerprint of PCV2i genomic nucleotides for the first time. In conclusion, this study illustrates the high frequency and evolutionary features of PCV2 in Jiangsu Province over the past few years. Full article
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