Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.2
3.0
Journals
Animals
Special Issues
Stem Cells in Animal Reproduction
share announcement

Stem Cells in Animal Reproduction

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: closed (15 November 2023) | Viewed by 2845

Special Issue Editor

Dr. Fabiana Fernandes Bressan

E-Mail Website
Guest Editor
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga 13635-000, Brazil
Interests: developmental biology cellular; epigenetic reprogramming; stem cells; cell therapy and gene therapy; induced reprogramming; in vitro gametogenesis; cultivated meat
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Stem cells are widely known for their high potential for self-renewal and differentiation and their vast range of possible applications in regenerative and translational medicine. Adult stem cells have already proven applicable in numerous cellular therapy protocols; however, the derivation and maintenance of pluripotent cells in domestic species are still challenging. The use of multipotent or pluripotent stem cells and their derivates (extracellular vesicles, recellularized scaffolds, etc.) may enable innovative possibilities in regenerative and reproductive veterinary medicine due to their ability to differentiate into a variety of other cell types or else to produce important signaling factors. Therefore, this Special Issue aims to congregate recent studies on the importance of the use of stem cells in animal reproduction, including possibilities of its use in regenerative protocols or else seeking the correction of infertility with recent advances in the generation of functional gametes in vitro, an essential contribution of stem cells for both the conservation of species in risk of extinction, and also for agricultural and biomedical applications.

Dr. Fabiana Fernandes Bressan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • iPS cells
  • biotechnology
  • regenerative medicine
  • reproduction

Published Papers (2 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

23 pages, 10897 KiB  
Article
Porcine Germ Cells Phenotype during Embryonic and Adult Development
by Amanda Soares Jorge, Kaiana Recchia, Mayra Hirakawa Glória, Aline Fernanda de Souza, Laís Vicari de Figueirêdo Pessôa, Paulo Fantinato Neto, Daniele dos Santos Martins, André Furugen Cesar de Andrade, Simone Maria Massami Kitamura Martins, Fabiana Fernandes Bressan and Naira Caroline Godoy Pieri
Animals 2023, 13(15), 2520; https://doi.org/10.3390/ani13152520 - 4 Aug 2023
Cited by 1 | Viewed by 1290
Abstract
Primordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in [...] Read more.
Primordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in domestic animals, e.g., swine, regarding the epigenetic and pluripotency profiles of PGCs during development. This study analyzed the expression of epigenetic and various pluripotent and germline markers associated with the development and differentiation of PGCs in porcine (pPGCs), aiming to understand the different gene expression profiles between the genders. The analysis of gonads at different gestational periods (from 24 to 35 days post fertilization (dpf) and in adults) was evaluated by immunofluorescence and RT-qPCR and showed phenotypic differences between the gonads of male and female embryos. In addition, the pPGCs were positive for OCT4 and VASA; some cells were H3k27me3 positive in male embryos and adult testes. In adults, the cells of the testes were positive for germline markers, as confirmed by gene expression analysis. The results may contribute to understanding the pPGC pathways during reproductive development, while also contributing to the knowledge needed to generate mature gametes in vitro. Full article
(This article belongs to the Special Issue Stem Cells in Animal Reproduction)
►▼ Show Figures

Graphical abstract

Review

Jump to: Research

24 pages, 1589 KiB  
Review
Avian iPSC Derivation to Recover Threatened Wild Species: A Comprehensive Review in Light of Well-Established Protocols
by Iara Pastor Martins Nogueira, Guilherme Mattos Jardim Costa and Samyra Maria dos Santos Nassif Lacerda
Animals 2024, 14(2), 220; https://doi.org/10.3390/ani14020220 - 10 Jan 2024
Viewed by 1125
Abstract
Induced pluripotent stem cells (iPSCs) were first generated by Yamanaka in 2006, revolutionizing research by overcoming limitations imposed by the use of embryonic stem cells. In terms of the conservation of endangered species, iPSC technology presents itself as a viable alternative for the [...] Read more.
Induced pluripotent stem cells (iPSCs) were first generated by Yamanaka in 2006, revolutionizing research by overcoming limitations imposed by the use of embryonic stem cells. In terms of the conservation of endangered species, iPSC technology presents itself as a viable alternative for the manipulation of target genetics without compromising specimens. Although iPSCs have been successfully generated for various species, their application in nonmammalian species, particularly avian species, requires further in-depth investigation to cover the diversity of wild species at risk and their different protocol requirements. This study aims to provide an overview of the workflow for iPSC induction, comparing well-established protocols in humans and mice with the limited information available for avian species. Here, we discuss the somatic cell sources to be reprogrammed, genetic factors, delivery methods, enhancers, a brief history of achievements in avian iPSC derivation, the main approaches for iPSC characterization, and the future perspectives and challenges for the field. By examining the current protocols and state-of-the-art techniques employed in iPSC generation, we seek to contribute to the development of efficient and species-specific iPSC methodologies for at-risk avian species. The advancement of iPSC technology holds great promise for achieving in vitro germline competency and, consequently, addressing reproductive challenges in endangered species, providing valuable tools for basic research, bird genetic preservation and rescue, and the establishment of cryobanks for future conservation efforts. Full article
(This article belongs to the Special Issue Stem Cells in Animal Reproduction)
►▼ Show Figures

Graphical abstract

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-2
Back to TopTop