Applications of Genome Editing, iPSCs and SCNT in Domestic Mammal Research

Dear Colleagues,

Using GE, iPSC, SCNT, and interspecies chimeric, we are routinely producing GE piPSC for improving disease resistance in pigs, generation of laboratory animals, and development of medical device source animals; and also using GFP-FMiPSC to complementation with target gene KO porcine blastocyst to elucidate or refer for interspecies chimeric issues before using human iPSC.

Current development in animal biotechnology including somatic cells nuclear transfer, induced pluripotent stem cell, gene-editing, etc., have promoted and facilitated livestock breeding, especially those popular e.g. pigs from conventional production to medical application, which including laboratory animals, therapeutics, medical devices, and possible xenotransplantation and tailor-made patient’s transplantable organs. The journal of “Animals” proposes a special issue on “Applications of Genome Editing, iPSCs and SCNT in Domestic Mammal Research”. As guest and co-guest editors of the journal, we have been impressed on your distinguished publication “XXXX……” and would like to invite you to submit your manuscripts, including original article, review paper or commentary, of the current study to this special issue to solid the progress and achievement domestic mammal or swine biotechnology and improve human life or health.

By using the gene-editing (GE) approach and induced pluripotent stem cell (iPSC), we routinely clone target gene-edited/knockout (KO) porcine iPSCs (piPSC) and knock-in (KI) βactin-GFP gene into Formosan macaque monkey iPSC (GFP-FMiPSC). These piPSCs can be used as nuclear donors for somatic cell nuclear transfer (SCNT) to generate gene KO blastocyst and further develop to disease resistance; may also become laboratory or medical device source pigs. Once gene KO porcine blastocysts can be developed, the specific organogenesis should be disabled to complement with GFP-FMiPSC. However, the efficiency of SCNT is very low especially iPSCs were used as nuclear donors, we currently are trying to improve the efficiency of SCNT by modifying the methods and using piPSC.  Furthermore, the complication of interspecies chimeric within porcine blastocysts and GFP-FMiPSC, has made us devote more to some basic issues before using porcine KO blastocyst and complementation with human iPSC.

If you are concerned or accept our invitation, please inform us and submit your manuscript before 20th September 2022 or let us know the feasible date of manuscript submission.

Dr. Chingfu Tu
Prof. Dr. Yu-Ten Ju
Guest Editors

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• cattle
• genome editing
• goat
• pig
• sheep