Microorganisms: A Way Forward for Sustainable Development?

A special issue of Applied Microbiology (ISSN 2673-8007).

Deadline for manuscript submissions: closed (31 December 2025) | Viewed by 2276

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School of Medicine, Department of Molecular Cell Biology, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea
Interests: microbial biotechnology
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Special Issue Information

Dear Colleagues,

The increasing cost of food, feeds, fuel, and fertilizers (4F) has created significant challenges in the 21th century. Climate change and geopolitical situations have further accentuated this problem to an alarming level where futuristic technology involving microbial biotechnology will play a key role in solving these problems and creating a sustainable circular bioeconomy. Microorganisms have already been exemplified to play a crucial role in the circular economy by creating biomass for food, feed, fuel and biofertilizer. Furthermore, microbes help to break down organic waste and persistent chemicals and convert it into valuable resources such as biogas, biofertilizers, and bio-based chemicals. However, the use of high-value microbials is often limited to the lab and/or pilot scale due to their limited efficiency or difficulties associated with obtaining regulatory approval. Microbial biotechnology and genetic engineering using cutting-edge technology can increase efficiency and enable the use of microbial potentials at an industrial scale to solve the crisis of 4F. This Special Issue of ‘Microrganisms’ focuses on 4F using wild-type or genetically engineered microbes to achieve a sustainable solution for 4F and create a circular economy.

You may choose our Joint Special Issue in Microorganisms.

Dr. Akhilesh Kumar Chaurasia
Guest Editor

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Keywords

  • microorganisms
  • microbial biotechnology
  • sustainability
  • genetically engineered microbes

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Published Papers (2 papers)

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Research

14 pages, 1253 KB  
Article
Antagonistic Activity of Streptomyces spp. Waksman & Henrici Isolated from Larrea tridentata (Sesse’ & Moc. Ex DC.) Coville and Rhizosphere Soil Against Fusarium spp.
by Servando H. Cantú-Bernal, Alonso A. Orozco-Flores, Víctor E. Aguirre-Arzola, Ricardo Gomez-Flores, Orquídea Pérez-González and Patricia Tamez-Guerra
Appl. Microbiol. 2026, 6(2), 33; https://doi.org/10.3390/applmicrobiol6020033 - 15 Feb 2026
Viewed by 641
Abstract
Agriculture is an essential activity in Mexico, representing the main source of income of numerous families. Crops are negatively affected by many diseases, particularly caused by phytopathogenic fungi, whose control by biological agents emerges as an advantageous alternative. The aim of the present [...] Read more.
Agriculture is an essential activity in Mexico, representing the main source of income of numerous families. Crops are negatively affected by many diseases, particularly caused by phytopathogenic fungi, whose control by biological agents emerges as an advantageous alternative. The aim of the present study was to evaluate the antagonistic activity of microorganisms isolated from Larrea tridentata L. (Sessé & Moc. Ex DC.) Coville leaves, stems, roots, and rhizospheric soil against Fusarium spp. and other phytopathogen fungi. We identified 54 microorganisms: 30 bacteria species and 24 actinobacteriota. Initial dual-confrontation experiments with phytopathogenic fungi determined the bacillus and actinobacteriota inhibited growth from 57 to 100% and 42 to 83%, respectively. Based on our initial results, selected isolates were confronted with Rhizoctonia sp. and two Fusarium spp. isolates (orchid and garlic isolates). All microorganisms inhibited Rhizoctonia, but only 13 bacillary bacteria and eight actinobacteriota isolates inhibited Fusarium and were selected for the third confrontation, in which firmicutes –Bacilli:Bacilliales- and actinobacteriota isolates inhibited Fusarium spp. growth from 55 to 92% and 14 to 74%, respectively. In addition, supernatant fluids from six selected actinobacteriota were evaluated, and the results determined that the strains OP-AGsD3, OP-AGsM4R7, and OP-AGsM1R5 possessed the highest antagonist activity against all Fusarium spp. isolates. Molecular identification analysis indicated that actinobacteriota belonged to the Streptomyces genus. Our results revealed the potential of native Streptomyces spp. from L. tridentata rhizosphere soil as biocontrol agents against phytopathogenic Fusarium spp. Full article
(This article belongs to the Special Issue Microorganisms: A Way Forward for Sustainable Development?)
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17 pages, 1157 KB  
Article
Isolation and Characterization of Phosphate-Solubilizing Rhizobacteria from Solanum tuberosum with Plant Growth-Promoting Activity
by Michel Leiva-Mora, Pamela Elizabeth Mera Guzmán, Rafael Isaías Mera-Andrade, Alicia Monserrath Zabala Haro, Luis Rodrigo Saa, Paúl Loján, Catherine Lizzeth Silva Agurto, Luis Fabián Salazar-Garcés, Betty Beatriz González Osorio, Dariel Cabrera Mederos and Orelvis Portal
Appl. Microbiol. 2026, 6(1), 8; https://doi.org/10.3390/applmicrobiol6010008 - 3 Jan 2026
Cited by 1 | Viewed by 1165
Abstract
Phosphate-solubilizing rhizobacteria associated with the Solanum tuberosum L. cultivar ‘Superchola’ were isolated and characterized to improve our understanding of plant growth promotion in agricultural systems. Bacteria were isolated by serial dilutions, and the morphology of the colonies was characterized on nutrient agar culture [...] Read more.
Phosphate-solubilizing rhizobacteria associated with the Solanum tuberosum L. cultivar ‘Superchola’ were isolated and characterized to improve our understanding of plant growth promotion in agricultural systems. Bacteria were isolated by serial dilutions, and the morphology of the colonies was characterized on nutrient agar culture medium. In addition, morphological identification was achieved by Gram staining. The ability to solubilize phosphate was assessed in Pikovskaya agar culture medium, while molecular identification involved the amplification of the partial 16S rRNA gene using the polymerase chain reaction. In the Píllaro canton, the highest number of colony-forming units per gram of soil was recorded at 9.72 × 109. Among the isolated strains, 62% exhibited circular morphology, 92% had a smooth texture, and 85% displayed entire margins. Notably, 83% of the isolates were Gram-negative, with 50% exhibiting a bacillary form. The most effective phosphate solubilizers were from the Mocha canton, particularly the isolate CC-FCAGP-BSF6, which showed superior solubilization capacity. Molecular identification revealed bacterial isolates from four genera, i.e., Bacillus, Pseudomonas, Lysinibacillus, and Paenibacillus. These strains exhibited significant phosphate solubilization in vitro and resulted in increased leaf area (0.21–0.49, p = 0.038), fresh mass (0.46–0.87, p = 0.014), dry mass (0.092–0.096, p = 0.047), and leaf area index (0.14–0.33, p = 0.026) in the S. tuberosum cultivar ‘Superchola’ in vitro plants. This study identifies bacterial species associated with the rhizosphere of S. tuberosum in Ecuador and highlights their potential for promoting plant growth and solubilizing phosphates. Full article
(This article belongs to the Special Issue Microorganisms: A Way Forward for Sustainable Development?)
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