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Biosensors
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Electrochemical Biosensor Applications in Agriculture, Environment and Health Systems
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Electrochemical Biosensor Applications in Agriculture, Environment and Health Systems

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (30 November 2025) | Viewed by 4260

Special Issue Editors

Dr. Fernando Javier Arévalo

E-Mail Website
Guest Editor
Grupo de Electroanalítica (GEANA), Departamento de Química, Instituto para el Desarrollo Agroindustrial y de la Salud UNRC-CONICET (IDAS), Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto 5800, Argentina
Interests: electrochemical sensors; immunosensors; mycotoxins; pesticides; photochemical methods
Dr. Adrián Marcelo Granero

E-Mail Website
Guest Editor
Grupo de Electroanalítica (GEANA), Departamento de Química, Instituto para el Desarrollo Agroindustrial y de la Salud UNRC-CONICET (IDAS), Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto 5800, Argentina
Interests: electrochemical sensors; molecularly imprinted polymer; antioxidants; drugs; enzymatic biosensors; herbicides

Special Issue Information

Dear Colleagues,

Electrochemical sensing play a crucial role in agriculture, environment, and health systems. Different types of materials are used for the construction of these electrochemical devices, which are responsible for the main stage of sensing the compound of interest (or groups of them). These materials can be biological (such as enzymes, antibodies, nucleic acids, tissues, organelles, whole cells, etc.) or synthetic (such as enzymes artificial, molecularly imprinted polymers, synthetic DNA and RNA, etc.). Both electrochemical biosensors and their artificial analogs have their strengths and weaknesses. The choice between one or the other, or a combination of both, depends on the specific needs of the use for which it will be built. Continuous innovation and interdisciplinarity in the field are promoting significant advances, making these technologies increasingly efficient and versatile.

In agriculture applications, these biosensors detect contaminants like pesticides, mycotoxins, and pathogens (and a great variety of compounds), ensuring food safety. Environmental monitoring utilizes these biosensors to track pollutants, such as heavy metals and organic compounds, promoting ecosystem health. In healthcare, they are vital for diagnosing numerous diseases, monitoring glucose levels, and detecting biomarkers, among other things.

Dr. Fernando Javier Arévalo
Dr. Adrián Marcelo Granero
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • biological materials
  • synthetic materials
  • agriculture systems
  • environmental systems
  • health systems

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Published Papers (3 papers)

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Research

15 pages, 2459 KB  
Article
Conductometric Chemosensor for Saccharides Based on Thin Films of Poly(3-Thienylboronic) Acid: Measurements of Transversal Resistance
by Berfinsu Kaya, Yulia Efremenko and Vladimir M. Mirsky
Biosensors 2025, 15(10), 679; https://doi.org/10.3390/bios15100679 - 9 Oct 2025
Viewed by 713
Abstract
Poly(3-thienylboronic acid) (PThBA) has recently been suggested as a conducting polymer with affinity for saccharides. In this study, thin films of this compound were deposited onto gold electrodes. The system obtained was studied as a possible chemical sensor. The measurements were performed by [...] Read more.
Poly(3-thienylboronic acid) (PThBA) has recently been suggested as a conducting polymer with affinity for saccharides. In this study, thin films of this compound were deposited onto gold electrodes. The system obtained was studied as a possible chemical sensor. The measurements were performed by impedance spectroscopy using potassium ferro/ferricyanide as a redox mediator. The thickness of the polymer and the deposition of the adhesive sublayer were optimized to achieve a compromise between the blocking of defects in the polymer layer and the unnecessary increase in the internal resistance of this conductometric sensor. A comparative study of the influence of fructose, glucose, and sorbitol on transversal polymer resistance was conducted. The binding constants for these saccharides were extracted from the concentration dependencies of sensor conductance. Among them, sorbitol showed the highest affinity with a binding constant up to ~15,000 L·mol−1, followed by fructose (~8700 L·mol−1) and glucose (~4500 L·mol−1). In order to exclude the contribution of the analyte tautomers on the obtained binding constants, measurements of ethylene glycol were also performed. The effects of pH and the redox state of PThBA on its affinity properties were studied, revealing higher affinities at alkaline pH and in oxidized state of the chemosensitive polymer. The developed system has the capacity to be applied in chemical sensors and virtual sensor arrays with electrical affinity control. Full article
(This article belongs to the Special Issue Electrochemical Biosensor Applications in Agriculture, Environment and Health Systems)
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13 pages, 2502 KB  
Article
Real-Time and Selective Detection of Pseudomonas aeruginosa in Beef Samples Using a g-C3N4-Doped Multimetallic Perovskite-Based Electrochemical Aptasensor
by Sarah S. Albalawi, Naeem Akhtar and Waleed A. El-Said
Biosensors 2025, 15(10), 634; https://doi.org/10.3390/bios15100634 - 23 Sep 2025
Cited by 1 | Viewed by 786
Abstract
The alarming rise in foodborne illnesses, particularly those associated with microbial contamination in meat products, presents a serious challenge to global food safety. Among these microbial threats, Pseudomonas aeruginosa (P. aeruginosa) poses a critical threat due to its biofilm-forming capability and [...] Read more.
The alarming rise in foodborne illnesses, particularly those associated with microbial contamination in meat products, presents a serious challenge to global food safety. Among these microbial threats, Pseudomonas aeruginosa (P. aeruginosa) poses a critical threat due to its biofilm-forming capability and prevalence in contaminated beef, highlighting its effective real-time detection. Herein, we report the fabrication of a novel electrochemical aptasensor based on multimetal perovskite (FeCoCuNiO) doped with urea-derived graphitic carbon nitride (g-C3N4), synthesized via a sol–gel combustion method. The FeCoCuNiO-g-C3N4 nanocomposite was then coated onto a graphitic pencil electrode and functionalized with a DNA-based aptamer specific towards P. aeruginosa. The resulting aptasensor exhibited a low detection limit of 3.03 CFU mL−1 with high selectivity and sensitivity, and was successfully applied to real-time detection of P. aeruginosa in food samples. To the best of our knowledge, this work presents the first FeCoCuNiO-g-C3N4-based aptasensor for bacterial detection, offering a promising platform for food safety assurance and public health protection. Full article
(This article belongs to the Special Issue Electrochemical Biosensor Applications in Agriculture, Environment and Health Systems)
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17 pages, 1576 KB  
Article
Design of an MIP-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples
by José Alberto Cabas Rodríguez, Fernando Javier Arévalo and Adrian Marcelo Granero
Biosensors 2025, 15(8), 544; https://doi.org/10.3390/bios15080544 - 19 Aug 2025
Cited by 4 | Viewed by 2102
Abstract
Paracetamol (PAR) is a common antipyretic and analgesic extensively used to treat cold and flu symptoms. It has been proven to be effective in headaches and relieving fever and pain. It is usually found as an over-the-counter drug, which has been associated with [...] Read more.
Paracetamol (PAR) is a common antipyretic and analgesic extensively used to treat cold and flu symptoms. It has been proven to be effective in headaches and relieving fever and pain. It is usually found as an over-the-counter drug, which has been associated with an increase in cases of poisoning due to overdose. Therefore, the development of new analytical tools for the detection of PAR at low concentrations in different samples is necessary. In this work, a Molecularly Imprinted Polymer (MIP)-based electrochemical sensor was designed for the selective and sensitive determination of PAR using a glassy carbon electrode (GCE) modified with a polymeric film obtained through the electropolymerization of o-aminophenol. A complete characterization based on electrochemical techniques, such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and scanning electron microscopy (SEM) was used to examine all steps involved in the construction of the MIP-based electrochemical sensor. In addition, all parameters affecting the MIP were optimized. As a result, the MIP-based electrochemical sensor showed a very low limit of detection (LOD) of 10 nM, with an analytical sensitivity of (3.4 ± 0.1) A M⁻¹. In addition, construction of the MIP-based electrochemical sensor showed highly reproducibility, expressed in terms of a variation coefficient lower than 4%. The MIP-based electrochemical sensor was successfully used in an assay for the determination of PAR in pharmaceutical products. The performance of the MIP-based electrochemical sensor was compared to High Performance Liquid Chromatography (HPLC) for the determination of PAR in pharmaceutical samples, showing excellent agreement between the two methodologies. A very important aspect of the developed sensor was its reusability for at least twenty times. The MIP-based electrochemical sensor is a reliable analytical tool for the determination of PAR. Full article
(This article belongs to the Special Issue Electrochemical Biosensor Applications in Agriculture, Environment and Health Systems)
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