Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
9.9
5.1
Journals
Cells
Special Issues
Nanoscopy in Cell Biology
share announcement

Nanoscopy in Cell Biology

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cellular Biophysics".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 6853

Special Issue Editors

Prof. Dr. József Mihály

E-Mail Website
Guest Editor
1. Biological Research Centre, Szeged, Hungary
2. Department of Genetics, University of Szeged (SZTE), Szeged, Hungary
Interests: developmental genetics; cytoskeleton; formins; nanoscopy; muscle development
Dr. Szilárd Szikora

E-Mail Website
Guest Editor
Biological Research Centre, Szeged, Hungary
Interests: cytoskeleton; nanoscopy; muscle morphogenesis

Special Issue Information

Dear Colleagues,

Recent developments in optical super-resolution microscopy allowed us to study living organisms at the nanoscale level. Although these approaches are far from being the easy part of our cell biology routine, nanoscopy methods became increasingly popular as the breakthrough in resolution quickly turned into exciting new discoveries in various fields of cell biology. For example, synaptic structure, nuclear pore complexes, centrosomes, neuronal cytoskeleton, nuclear architecture, etc. were studied in unprecedented detail, leading to valuable novel structural and mechanistic insights. Despite the remarkable progress over the past decade, it has also become apparent that to fully exploit the capabilities of these nanoscopies, we need to find solutions for the poor temporal and axial resolution, multiplexing, and the limited efficiency of labeling, to mention a few key concerns. This Special Issue aims at reviewing the instrumental role of super-resolution imaging in improving our understanding of fundamental subcellular constituents. In addition, we would like to discuss the current limitations and the potential future elucidations that may provide real molecular resolution for cell biologists.

We welcome original research articles and review papers as well, and we look forward to receiving your contributions.

Prof. Dr. József Mihály
Dr. Szilárd Szikora
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanoscopy
  • super-resolution microscopy
  • single-molecule localization
  • STED
  • SIM
  • STORM
  • PALM
  • DNA-PAINT
  • MINFLUX

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

13 pages, 2283 KiB  
Article
Neurofilament Levels in Dendritic Spines Associate with Synaptic Status
by Clara-Marie Gürth, Maria Augusta do Rego Barros Fernandes Lima, Victor Macarrón Palacios, Angel Rafael Cereceda Delgado, Jasmine Hubrich and Elisa D’Este
Cells 2023, 12(6), 909; https://doi.org/10.3390/cells12060909 - 15 Mar 2023
Cited by 5 | Viewed by 2242
Abstract
Neurofilaments are one of the main cytoskeletal components in neurons; they can be found in the form of oligomers at pre- and postsynapses. How their presence is regulated at the postsynapse remains largely unclear. Here we systematically quantified, by immunolabeling, the occurrence of [...] Read more.
Neurofilaments are one of the main cytoskeletal components in neurons; they can be found in the form of oligomers at pre- and postsynapses. How their presence is regulated at the postsynapse remains largely unclear. Here we systematically quantified, by immunolabeling, the occurrence of the neurofilament isoform triplet neurofilament light (NFL), medium (NFM), and heavy (NFH) at the postsynapse using STED nanoscopy together with markers of synaptic strength and activity. Our data show that, within dendritic spines, neurofilament isoforms rarely colocalize with each other and that they are present to different extents, with NFL being the most abundant isoform. The amount of the three isoforms correlates with markers of postsynaptic strength and presynaptic activity to varying degrees: NFL shows the highest correlation to both synaptic traits, suggesting its involvement in synaptic response, while NFM exhibits the lowest correlations. By quantifying the presence of neurofilaments at the postsynapse within the context of the synaptic status, this work sheds new light on the regulation of synaptic neurofilaments and their possible contribution to synaptopathies. Full article
(This article belongs to the Special Issue Nanoscopy in Cell Biology)
►▼ Show Figures

Figure 1

13 pages, 3255 KiB  
Article
Megalin Orchestrates FcRn Endocytosis and Trafficking
by Eileen Dahlke, Yaman Anan, Lea Maximiliane Klie, Ariane Elisabeth Hartkopf and Franziska Theilig
Cells 2023, 12(1), 53; https://doi.org/10.3390/cells12010053 - 22 Dec 2022
Cited by 1 | Viewed by 1933
Abstract
The neonatal Fc receptor (FcRn) is highly expressed in the renal proximal tubule and is important for the reclamation of albumin by cellular transcytosis to prevent its loss in the urine. The initial event of this transcellular transport mechanism is the endocytosis of [...] Read more.
The neonatal Fc receptor (FcRn) is highly expressed in the renal proximal tubule and is important for the reclamation of albumin by cellular transcytosis to prevent its loss in the urine. The initial event of this transcellular transport mechanism is the endocytosis of albumin by the apical scavenger receptors megalin and cubilin. An interaction of megalin and FcRn was postulated, however, evidence is still missing. Similarly, the intracellular trafficking of FcRn remains unknown and shall be identified in our study. Using a Venus-based bimolecular fluorescence complementation system, we detected an interaction between megalin and FcRn in the endosomal compartment, which significantly increased with the induction of endocytosis using albumin or lactoglobulin as a ligand. The interaction between megalin and FcRn occurred at a neutral and acidic pH between the extracellular domains of both proteins. Amnionless, another transmembrane acceptor of cubilin, revealed no interaction with FcRn. With the induction of endocytosis by albumin or lactoglobulin, super resolution microscopy demonstrated a redistribution of megalin and FcRn into clathrin vesicles and early endosomes. This trafficking into clathrin vesicles was impaired in megalin-deficient cells upon albumin-induced endocytosis, supporting the role of megalin in FcRn redistribution. Our results indicate that megalin and FcRn specifically bind and interact within their extracellular domains. The availability of megalin is necessary for the redistribution of FcRn. Megalin, therefore, orchestrates FcRn endocytosis and intracellular trafficking as an early event intranscytosis. Full article
(This article belongs to the Special Issue Nanoscopy in Cell Biology)
►▼ Show Figures

Figure 1

14 pages, 1939 KiB  
Article
Application of Lacunarity for Quantification of Single Molecule Localization Microscopy Images
by Bálint Barna H. Kovács, Dániel Varga, Dániel Sebők, Hajnalka Majoros, Róbert Polanek, Tibor Pankotai, Katalin Hideghéty, Ákos Kukovecz and Miklós Erdélyi
Cells 2022, 11(19), 3105; https://doi.org/10.3390/cells11193105 - 2 Oct 2022
Cited by 2 | Viewed by 1841
Abstract
The quantitative analysis of datasets achieved by single molecule localization microscopy is vital for studying the structure of subcellular organizations. Cluster analysis has emerged as a multi-faceted tool in the structural analysis of localization datasets. However, the results it produces greatly depend on [...] Read more.
The quantitative analysis of datasets achieved by single molecule localization microscopy is vital for studying the structure of subcellular organizations. Cluster analysis has emerged as a multi-faceted tool in the structural analysis of localization datasets. However, the results it produces greatly depend on the set parameters, and the process can be computationally intensive. Here we present a new approach for structural analysis using lacunarity. Unlike cluster analysis, lacunarity can be calculated quickly while providing definitive information about the structure of the localizations. Using simulated data, we demonstrate how lacunarity results can be interpreted. We use these interpretations to compare our lacunarity analysis with our previous cluster analysis-based results in the field of DNA repair, showing the new algorithm’s efficiency. Full article
(This article belongs to the Special Issue Nanoscopy in Cell Biology)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop